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Research article

lncRNA ABHD11-AS1 drives gastric cancer development by targeting miR-1301-3p/PDPK1 signaling

hairong xin, zhefeng yan, jie cao
DOI: 10.21203/rs.2.12814/v1

Abstract

Background

Long noncoding RNAs (lncRNAs) has long been reported to associate with multitudinous kinds of cancer. The ABHD11-AS1, a newly identified lncRNAs, has been demonstrated as a new marker of gastric cancer (GC). In this study, a significant upregulation of ABHD11-AS1 was observed in GC, compared to adjacent normal tissues.

Method

Immunohistochemical analysis for ABHD11-AS1, 3-phosphoinositide dependent protein kinase 1 (PDPK1), miR-1301-3p was performed on gastric tumor and adjacent normal tissues. The gene expression was evaluated by qRT-PCR. The proliferation was assayed by CCK8 and Annexin V/PI assay.

Results

ABHD11-AS1 is highly expressed in GC tissues compared with adjacent normal tissues(p<0.01) Knock-down of ABHD11-AS1 could suppress the aberrant proliferation of GC cells compared with si-Scramble(p<0.01). LncRNA-ABHD11-AS1 served as a sponge for miR-1301-3p in GC cell. miR-1301-3p/ABHD11-AS1 regulated the expression of PDPK1 in GC cells compared with control p<0.01). miR-1301-3p/ ABHD11-AS1 co-mediated proliferation and apoptosis of GC cell compared with si-Scramble/Mock and si-ABHD11-AS1/Mock (p<0.01)

Conclusion

PDPK1 was a downstream target of miR-133a-3p and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of PDPK1in gastric cancer cells.

Key

lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1

Keywords
lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1

Figures

Background

Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

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    On 06 Aug, 2019

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  • First submitted

    On 24 Jul, 2019

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Preprint: Please note that this article has not completed peer review.
Research article

lncRNA ABHD11-AS1 drives gastric cancer development by targeting miR-1301-3p/PDPK1 signaling

hairong xin, zhefeng yan, jie cao

STATUS: In Review

Comments: 0
PDF Downloads: 0
HTML Views: 4

Integrity Check:

  • Article

  • Peer Review Timeline

  • Related Articles

  • Comments

Abstract

Background

Long noncoding RNAs (lncRNAs) has long been reported to associate with multitudinous kinds of cancer. The ABHD11-AS1, a newly identified lncRNAs, has been demonstrated as a new marker of gastric cancer (GC). In this study, a significant upregulation of ABHD11-AS1 was observed in GC, compared to adjacent normal tissues.

Method

Immunohistochemical analysis for ABHD11-AS1, 3-phosphoinositide dependent protein kinase 1 (PDPK1), miR-1301-3p was performed on gastric tumor and adjacent normal tissues. The gene expression was evaluated by qRT-PCR. The proliferation was assayed by CCK8 and Annexin V/PI assay.

Results

ABHD11-AS1 is highly expressed in GC tissues compared with adjacent normal tissues(p<0.01) Knock-down of ABHD11-AS1 could suppress the aberrant proliferation of GC cells compared with si-Scramble(p<0.01). LncRNA-ABHD11-AS1 served as a sponge for miR-1301-3p in GC cell. miR-1301-3p/ABHD11-AS1 regulated the expression of PDPK1 in GC cells compared with control p<0.01). miR-1301-3p/ ABHD11-AS1 co-mediated proliferation and apoptosis of GC cell compared with si-Scramble/Mock and si-ABHD11-AS1/Mock (p<0.01)

Conclusion

PDPK1 was a downstream target of miR-133a-3p and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of PDPK1in gastric cancer cells.

Key

lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1

Figures

Background

Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

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