Preprint: Please note that this article has not completed peer review.

Effects of initial periodontal therapy on the prevalence of Epstein-Barr virus DNA and Porphyromonas gingivalis in Japanese chronic periodontitis patients

Ayako Kato, Yorinobu Ikeda, Kenichi Imai, Yorimasa Ogata
DOI: 10.21203/rs.2.11335/v1

Abstract

Background

Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis, we used subgingival plaque samples from chronic periodontitis (CP) patients.

Methods

Seventeen CP patients were recruited and measured periodontal clinical parameters such as probing pocket depth (PD) and bleeding on probing (BOP), and subgingival plaque samples were collected from two periodontal sites with PD of <3 mm (healthy sites: HS) or >5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis.

Results

EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) in the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) in the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) in the HS, and 9 (52.9%) and 10 (58.8%) in the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12; 70.6%) were significantly decreased after IPT (6; 35.3%).

Conclusion

These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis. Further research would be necessary for improving the periodontal treatment strategy.

Keywords
Epstein-Barr virus, initial periodontal therapy, Porphyromonas gingivalis, periodontitis, prevalence

Background

Methods

Results

Discussion

Abbreviations

Declarations

References

Tables

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    Received 25 Jul, 2019

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    On 16 Jul, 2019

  • Reviewer #1 agreed

    On 14 Jul, 2019

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  • Submission checks complete

    On 10 Jul, 2019

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    On 10 Jul, 2019

  • Editor invited

    On 09 Jul, 2019

  • First submitted

    On 26 Jun, 2019

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Preprint: Please note that this article has not completed peer review.

Effects of initial periodontal therapy on the prevalence of Epstein-Barr virus DNA and Porphyromonas gingivalis in Japanese chronic periodontitis patients

Ayako Kato, Yorinobu Ikeda, Kenichi Imai, Yorimasa Ogata

STATUS: In Review

Comments: 0
PDF Downloads: 0
HTML Views: 4

Integrity Check:

  • Article

  • Peer Review Timeline

  • Related Articles

  • Comments

Abstract

Background

Initial periodontal therapy (IPT) is cornerstone of periodontal therapy and the first step to control of periodontal risk factors. Scaling and root planing are used to treat root surface irregularities and remove virulent factors caused by periodontal pathogens. This procedure also incorporated into periodontal surgery. To elucidate the effects of IPT on prevalence of Epstein-Barr virus (EBV) DNA and Porphyromonas gingivalis, we used subgingival plaque samples from chronic periodontitis (CP) patients.

Methods

Seventeen CP patients were recruited and measured periodontal clinical parameters such as probing pocket depth (PD) and bleeding on probing (BOP), and subgingival plaque samples were collected from two periodontal sites with PD of <3 mm (healthy sites: HS) or >5 mm (periodontitis sites: PS) at first visit and after IPT. Plaque samples were subjected to a real-time PCR to detect EBV DNA and P. gingivalis.

Results

EBV DNA and P. gingivalis were detected 9 (52.9%) and 14 (82.3%) in the subgingival samples from HS, and 13 (76.5%) and 14 (82.3%) in the PS at first visit. After IPT, number of detections of EBV DNA and P. gingivalis were decreased to 5 (29.4%) and 13 (76.5%) in the HS, and 9 (52.9%) and 10 (58.8%) in the PS. Significant improvements in PD and BOP were observed after IPT in PS. Coexistence of EBV DNA and P. gingivalis in the subgingival samples from PS at first visit (12; 70.6%) were significantly decreased after IPT (6; 35.3%).

Conclusion

These results suggest that the IPT was effective in improvement of clinical parameters such as PD and BOP and reducing the coexistence of EBV and P. gingivalis in the subgingival plaque from PS. However, IPT could not eradicate the EBV and P. gingivalis. Further research would be necessary for improving the periodontal treatment strategy.

Background

Methods

Results

Discussion

Abbreviations

Declarations

References

Tables

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