Preprint: Please note that this article has not completed peer review.
Method Article

Live imaging of mouse embryos during pre-implantation and peri-implantation development

Neophytos Christodoulou, Antonia Weberling, Magdalena Zernicka-Goetz

Abstract

Live visualisation of embryo development is a powerful tool for scientists to understand how morphogenetic events shape the embryo. Here, we report on a culturing technique that allows live imaging of pre- and peri-implantation mouse embryos throughout the process of blastocyst to egg cylinder transition, enabling single cell tracking and delineation of the tissue dynamics accompanying this morphogenetic stage. At the same time, this protocol can be used for pharmacological manipulations of mouse embryos.

Keywords
Developmental Biology, Live imaging, Mouse embryogenesis

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Preprint: Please note that this article has not completed peer review.
Method Article

Live imaging of mouse embryos during pre-implantation and peri-implantation development

Neophytos Christodoulou, Antonia Weberling, Magdalena Zernicka-Goetz

STATUS: Posted

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Abstract

Live visualisation of embryo development is a powerful tool for scientists to understand how morphogenetic events shape the embryo. Here, we report on a culturing technique that allows live imaging of pre- and peri-implantation mouse embryos throughout the process of blastocyst to egg cylinder transition, enabling single cell tracking and delineation of the tissue dynamics accompanying this morphogenetic stage. At the same time, this protocol can be used for pharmacological manipulations of mouse embryos.

Figures

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Anticipated Results

References

Learn more about our company.