Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis
Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens.
Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.
Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.
Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Cryptococcosis is caused by Cryptococcus, an opportunistic pathogen. C. gattii and C. neoformans are responsible for almost all cryptococcal infections in humans. People with low immunity have a high probability of being infected with Cryptococcus; for example, HIV patients and patients with long-term use of glucocorticoids, immunosuppressants, broad-spectrum antibiotics, and anti-tumor drugs[1–2]. All organs of humans can be infected with Cryptococcus. Without complement and anti-cryptococcus growth factors in cerebrospinal fluid (CSF), cryptococcal meningitis (CM) is the main clinical manifestation of the cryptococcal infection in the central nervous system[3]. Approximately, one million individuals die of cryptococcosis each year worldwide[4]. Even in areas where antiretroviral therapy is available, the fatality rate of CM is high, and only about 50% of the patients survive within 6 months[3].
Thus, cryptococcosis has become a serious global public health problem. However, the cryptococcal infection is short of specificity with diverse clinical manifestations. Cryptococcosis is frequently misdiagnosed at the early stage[5–7]. Lateral flow immunochromatographic assay (LFA) is a rapid diagnostic method for the quantitative or qualitative detection of analytes in complex mixtures providing results within 5–30 min[8].
The diagnosis of cryptococcosis relies on the histopathological examination, pathogenic smear, and culture of pus puncture specimens in the lesion. These methods have the advantage of high specificity, but the sensitivity is low. Moreover, these tests are time-consuming and require auxiliary equipment[9]. LFA can detect samples without special auxiliary equipment, which can also be used for the determination of single samples and preserve the results of the test. As a rapid diagnostic method, LFA has high specificity and sensitivity under the premise of rapid reaction. The USA Food and Drug Administration has approved LFA as a semi-quantitative tool for the rapid detection of cryptococcal capsular polysaccharide antigen in the serum or CSF[10]. Thus, this diagnostic technology has been adopted by most countries for the detection of cryptococcosis. The application of LFA for the rapid diagnosis of cryptococcosis dramatically shortens the time of detection. Furthermore, it provides a basis for the rapid faster diagnosis of cryptococcal disease and guidelines for future treatment. Herein, we collected relevant articles for the meta-analysis to assess LFA for the diagnostic accuracy of cryptococcosis.
Four investigators systematically searched all the articles about the cryptococcus and LFA before July 2019 in the Embase, PubMed, Web of Science, and Cochrane Library databases. We used the keywords “cryptococcus, torula, filobasidiella” and “lateral flow immunochromatographic assay, LFA, colloidal gold immunochromatography: for advanced search. Geographical restrictions were not applied in these articles.
Two investigators systematically screened all of the articles by pre-established screening criteria. The inclusion criteria were as follows: (1) Studies published in English. (2) The purpose of the study was related to LFA and cryptococcosis. (3) Studies limited to original research. (4) Studies related to diagnostics. (5) Data can be extracted to construct fourfold tables. The exclusion criteria were as follows: (1) Duplicate studies, abstracts, conference abstracts, case reports, reviews, editorials. (2) Studies without a reference standard or a detailed number of samples. (3) Samples not from humans. (4) LFA as the reference standard.
In the process of carefully reading the included articles, the investigators simultaneously extracted related data from the studies, including the name of the first author, year of article, study design, geographical distribution of strains, patient population, reference standard, sample type, true positive (TP), false positive (FP), true negative (TN), and false-negative (FN). The process of extracting data is carried out independently by the investigators, and finally, the synthesis results were compared.
We used the Quality Assessment of Diagnostic Accuracy Study (QUADAS–2) guidelines[11] to assess the quality of included studies. Then, we analyzed the risk of bias and applicability concerns by Review Manager 5.0, including patient selection, reference standard, index test, flow, and timing. If the assessment results conflicted, the investigators reviewed the original studies, and a third investigator would intervene to achieve consensus.
We analyzed the extracted data, such as specificity, sensitivity, negative likelihood ratio (NLR), positive likelihood ratio (PLR), and diagnostic odds ratio (DOR), from the included studies using meta-DiSc 1.4 software. Also, we analyzed the summary receiver operating characteristic (SROC) curve and calculated the area under the curve (AUC). According to the sample types, these studies were analyzed by different methods. Due to the lack of adequate data on urine and other samples in the included articles, these samples were analyzed by Review Manager 5.0 software for sensitivity and specificity. Finally, publication bias was evaluated by Stata12.0 software.
A total of 167 publications were retrieved, which decreased to 82 after excluding the duplicates. Also, 18 studies were excluded after screening the abstracts. After full-text review, we included 15 qualified articles[9–10,12–24].
Fifteen studies were published between 2011 and 2019. 13/15 articles reported data from serum samples, seven collected CSF samples, two contained urine samples, and one contained the samples of fingerprick capillary blood and whole venous blood. A total of 9312 samples were included in the meta-analysis, with an average of 620 (range 59–3447) samples. Table 1 summarizes the characteristics of these studies.
We assessed the quality of 15 articles using Review Manager 5.3. (Fig. 1)
We classified the studies into different categories due to the different sample types.
For serum specimens, the merged sensitivity and specificity values were 0.98 (95% CI: 0.96–0.99) and 0.98 (95% CI: 0.97–0.98), respectively. The average PLR of LFA in the serum was 45.05 (95% CI: 26.22–77.40) and the NLR was 0.04 (95% CI: 0.02–0.10). The merged DOR was 1574.65 (95% CI: 730.16–3395.87) and AUC was 0.9766. The results are shown in Figs. 2, 4A.
For CSF specimens, the merged sensitivity and specificity values were 0.99 (95% CI: 0.98–0.99) and 0.99 (95% CI: 0.99 to 0.99), respectively. The average PLR of LFA in CSF was 93.89 (95% CI: 53.54–164.64) and the NLR was 0.03 (95% CI: 0.01–0.07). The merged DOR was 3864.72 (95% CI: 1308.89–11411.28) and AUC was 0.9983. The results are shown in Figs. 3, 4B.
For other samples, the results of sensitivity and specificity are shown in Figure 5.
In this meta-analysis, the data of serum and CSF samples were tested by Stata 12.0 for publication bias. Deek’s funnel plot asymmetry test was used to assess the potential published bias in the included studies. The results of serum and CSF samples indicated that there was no obvious publication bias (Fig. 6).
Cryptococcosis is a disease with a high mortality rate. Therefore, a rapid diagnosis of cryptococcal infection is necessary for patients presenting appropriate clinical symptoms. A comprehensive search with stringent screening criteria retrieved 15 articles eligible for inclusion in the study. These 15 articles encompassed 3901 serum samples, 4403 CSF samples, 1125 urine samples, 1163 venous whole blood samples, and 1163 fingerprick capillary blood samples.
The results in meta-analysis showed that the combined sensitivity of LFA in serum and CSF was 0.98 (0.96–0.99) and 0.99 (0.98–0.99); specificity was 0.98 (0.97–0.98) and 0.99 (0.99–0.99); DOR was 1574.65 (730.16–3395.87) and 2509.29 (184.18–34187.48); SROC AUC was 0.9962 and 0.9983, respectively.
Among these indexes, the PLR of the serum and CSF was >10, while the NLR was <0.1. The SROC AUC of the serum and CSF was close to 1. The SROC curve was close to the upper left corner, which indicated that the area under the curve was large. Both the AUCs were >0.9, indicating that LFA had a relatively high overall diagnostic accuracy for serum and CSF. The DOR of serum and CSF was significant, indicating that the correct diagnosis is far larger than the wrong diagnosis. In conclusion, LFA has a high degree of accuracy in the diagnosis of serum and CSF.
The current analysis of these articles revealed several factors that can explain the observed heterogeneity: the differences in the reference methods in the studies; the same reference standard was not used in the study for identification; the interpretation of the results in LFA and reference methods may cause the artificial error.
Nevertheless, the current study has some limitations. Firstly, we collected all the relevant articles; however, it was difficult to ensure that no publication was missing. Secondly, we only included the articles published in the English language, which may contribute to bias. Thirdly, our study only included the articles from inception to August 2019. The difference in the reference standard might also lead to the heterogeneity of the included studies. Finally, meta-analyses of LFA for the diagnosis of cryptococcosis, only until 2015, were included. Thus, we could comprehensively analyze the accuracy of the LFA diagnosis of the cryptococcal infection.
In summary, our meta-analysis indicated that LFA tested in serum and CSF has high diagnostic accuracy in the diagnosis of cryptococcal infection for high-risk patients, such as HIV-infected patients. LFA performed in urine, or other samples could be a screening tool for the early diagnosis of cryptococcal infection; however, additional studies are required for the substantiation of these results.
Not applicable.
No funding was used to support this study.
All data generated or analyzed during this study are included in this published article and its supplementary information files.
Xu-Guang Guo conceived and designed the experiments. Li-Min Xie, Geng-ling Lin, Hao-Neng Dong and Ying-Xia Liao analyzed the data and made the tables. Li-Min Xie, Ye-Ling Liu and Jian-Feng Qin contributed to the production of figures by the analysis tools. All authors participated in the writing, reading, and revising of the manuscript and approved the final version of the manuscript.
Not applicable.
Not applicable.
The authors declare that they have no competing interests.
AUC: calculated the area under the curve; CSF: cerebrospinal fluid; CM: cryptococcal meningitis; DOR: diagnostic odds ratio; FP: false positive; FN: false negative; HIV: hunman immunodeficiency virus; LFA: Lateral flow immunochromatographic assay; NLR: Negative likelihood ratio; PLR: Positive likelihood ratio; QUADAS: Quality Assessment of Diagnostic Accuracy Study; SROC: Summary receiver operating characteristic; TP: true positive; TN: true negative;
[1]Gibson J F, Johnston S A. Immunity to Cryptococcus neoformansand C.gattii during cryptococcosis[J]. Fungal Genet Biol, 2015, 78:76–86.
[2]Xu J, Eastman A J, Flaczyk A, et al. Disruption of early tumor necrosis dactor alpha signaling prevents classic alactivation of dendritic cells in lung-associated lymph nodes and development of protective immunity against cryptococcal infection [J]. MBio, 2016, 7 (4): e00510–00516.
[3]Brown G D, Denning D W, Levitz S M. Tackling human fungal infections[J]. Sci, 2012, 336(6082):647.
[4]Fang W, Fa Z, Liao W. Epidemiology of Cryptococcus and cryptococcosis in China[J]. Fungal Genet Biol, 2015,78:7–15.
[5]Yoo SD, Worodria W, Med M, The prevalence and clinical course of HIV-associated pulmonary cryptococcosis in Uganda[J]. J Acquir Immune Defic Syndr 2010; 54:269–74.
[6]Jarvis JN, Wainwright H, Harrison TS, Pulmonary cryptococcosis misdiagnosed as smear-negative pulmonary tuberculosis with fatal consequences[J]. Int J Infect Dis 2010;14S:e310–12.
[7]Brizendine KD, Baddley JW, Pappas PG. Pulmonary cryptococcosis[J]. Semin Respir Crit Care Med 2011;32:727–34.
[8]Kozel T R, Bauman S K. CrAg lateral flow assay for cryptococcosis[J]. Expert opinion on medical diagnostics, 2012, 6(3): 245–251.
[9]Drain P K, Hong T, Krows M, et al. Validation of clinic-based cryptococcal antigen lateral flow assay screening in HIV-infected adults in South Africa[J]. Sci. Rep, 2019, 9(1).
[10]Mcmullan B J, Catriona H, Sorrell T C, et al. Clinical Utility of the Cryptococcal Antigen Lateral Flow Assay in a Diagnostic Mycology Laboratory[J]. PLoS ONE, 2012, 7(11):e49541.
[11]Lan W, Yong Z, Xian-tao Z. The QUADAS–2 Tool for the Quality Assessment of Diagnostic Accuracy Study:an Introduction[J]. J Hub Uni Med, 2013, 32(03):201–208.
[12]Lindsley M D, Mekha N, Baggett H C, et al. Evaluation of a Newly Developed Lateral Flow Immunoassay for the Diagnosis of Cryptococcosis[J]. Clin Inf Dis, 2011, 53(4):321–325.
[13]Binnicker M J, Jespersen D J, Bestrom J E, Rollins LO. Comparison of Four Assays for the Detection of Cryptococcal Antigen[J]. Clin Vac Immunol, 2012, 19(12): 1988–1990.
[14]Escandón, Patricia, Lizarazo J, Agudelo C I, et al. Evaluation of a rapid lateral flow immunoassay for the detection of cryptococcal antigen for the early diagnosis of cryptococcosis in HIV patients in Colombia[J]. Med Mycol, 2013, 51(7): 765–768.
[15]Hansen J, Slechta E S, Gates-Hollingsworth M A, et al. Large-Scale Evaluation of the Immuno-Mycologics Lateral Flow and Enzyme-Linked Immunoassays for Detection of Cryptococcal Antigen in Serum and Cerebrospinal Fluid[J]. Clin Vac Immunol, 2013, 20(1):52–55.
[16]Rugemalila J, Maro V P, Kapanda G, et al. Cryptococcal antigen prevalence in HIV-infected Tanzanians: a cross-sectional study and evaluation of a point-of-care lateral flow assay[J]. Trop Med Int Health, 2013, 18(9): 1075–1079.
[17]Boulware D R, Rolfes M A, Rajasingham R, et al. Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast[J]. Emerg Inf Dis, 2014, 20(1):45–53.
[18]Lourens A, Jarvis J N, Meintjes G, et al. Rapid Diagnosis of Cryptococcal Meningitis by Use of Lateral Flow Assay on Cerebrospinal Fluid Samples: Influence of the High-Dose “Hook” Effect[J]. J. Clin Microbiol, 2014, 52(12): 4172–4175.
[19]Diane Rivet-Dañon, Guitard J, Grenouillet, Frédéric, et al. Rapid diagnosis of cryptococcosis using an antigen detection immunochromatographic test[J]. J Inf, 2015, 70(5):499–503.
[20]Suwantarat N, Dalton J B, Lee R, et al. Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens[J]. Diagnostic Microbiol Inf Dis, 2015, 82(1):54–56.
[21]Jitmuang A, Panackal A A, Williamson P R, et al. Performance of the Cryptococcal Antigen Lateral Flow Assay in Non-HIV Related Cryptococcosis[J]. J clin microbiol, 2015, 54(2):460–463.
[22]Cáceres Diego H, Alejandra Z, Tabares ángela M, et al. Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia[J]. Rev Do Ins Med Trop São Paulo, 2017, 59(0):e76.
[23]Frola C, Guelfand L, Blugerman G, et al. Prevalence of cryptococcal infection among advanced HIV patients in Argentina using lateral flow immunoassay[J]. PloS One, 2017, 12(6):e0178721.
[24]Elvis T, Charles K, Leonella M, et al. Cryptococcal Antigen Screening in Asymptomatic HIV-Infected Antiretroviral Naïve Patients in Cameroon and Evaluation of the New Semi-Quantitative Biosynex CryptoPS Test[J]. Fron Microbiol, 2018, 9:409.
Table1. Characteristics of the included studies (n = 15).
Reference no. |
First author |
Year |
Geographical distribution of strains |
Study design |
Patient population |
Sample type(s) |
Sample size |
Reference standard |
1 |
Lindsley |
2011 |
Thailand |
prospective |
HIV |
Serum Urine |
538 |
EIA |
2 |
Binnicker |
2012 |
USA |
prospective&retrospective |
SC |
Serum |
634 |
LA |
3 |
McMullan |
2012 |
Australia |
retrospective |
SC |
Serum |
106 |
Comprehensive reference3 |
4 |
Escandón |
2013 |
Colombia |
retrospective |
HIV |
Serum |
421 |
LA |
5 |
Hansen |
2013 |
USA |
prospective |
SC |
Serum CSF |
1000 |
EIA |
6 |
Rugemalila |
2013 |
Tanzania |
prospective |
SC |
Serum |
319 |
LA |
7 |
Boulware |
2014 |
Uganda& South Africa |
prospective&retrospective |
HIV SM |
CSF |
666 |
Culture |
8 |
Lourens |
2014 |
South Africa |
prospective |
HIV SM |
CSF |
465 |
Culture/LA |
9 |
Rivet-Dañon |
2015 |
France |
prospective&retrospective |
IFI1 SC HIV |
Serum CSF |
292 |
LA |
10 |
Suwantarat |
2015 |
America |
retrospective&prospective |
SC |
Serum CSF |
1047 |
EIA/Enhanced reference4 |
11 |
Jitmuang |
2016 |
America |
retrospective |
HIV-N |
Serum CSF |
59 |
LA |
12 |
Cáceres |
2017 |
Colombia |
retrospective |
CIB2 |
Serum CSF |
83 |
LA |
13 |
Frola |
2017 |
Argentina |
prospective |
HIV |
Serum |
123 |
Comprehensive reference5 |
14 |
Temfack |
2018 |
Cameroon |
prospective |
HIV |
Serum |
186 |
EIA |
15 |
Drain |
2019 |
South Africa |
prospective |
HIV |
VWB FCB Urine |
3447 |
EIA/Combined reference6 |
HIV, hunman immunodeficiency virus; SC, suspected cryptococcosis; SM, suspected meningitis; HIV-N,HIV-negative; CSF,cerebrospinal fluid; VWB,venous whole blood; FCB,fingerprick capillary blood; LA, latex agglutination method; EIA, enzyme-linked immunoassay; 1:patients proven or probable invasive fungal infection other than cryptococcosis; 2:patients with or without diagnosis of cryptococcosis were randomly selected from a collection of biological samples stored in the CIB’s biobank; 3:Cryptococcosis was proven if the organism was detected by one or more of culture, histopathology or molecular tests; 4:An enhanced reference method includes data from histopathology, cytopathology, fungal culture, and patient clinical history in addition to EIA results; 5:Pathogen identification of isolates from positive blood cultures was performed using standard microbiology methods (morphological and biochemical tests); 6:A combined reference standard for either a positive CrAg EIA or latex agglutination test.
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Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis
Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens.
Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.
Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.
Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Cryptococcosis is caused by Cryptococcus, an opportunistic pathogen. C. gattii and C. neoformans are responsible for almost all cryptococcal infections in humans. People with low immunity have a high probability of being infected with Cryptococcus; for example, HIV patients and patients with long-term use of glucocorticoids, immunosuppressants, broad-spectrum antibiotics, and anti-tumor drugs[1–2]. All organs of humans can be infected with Cryptococcus. Without complement and anti-cryptococcus growth factors in cerebrospinal fluid (CSF), cryptococcal meningitis (CM) is the main clinical manifestation of the cryptococcal infection in the central nervous system[3]. Approximately, one million individuals die of cryptococcosis each year worldwide[4]. Even in areas where antiretroviral therapy is available, the fatality rate of CM is high, and only about 50% of the patients survive within 6 months[3].
Thus, cryptococcosis has become a serious global public health problem. However, the cryptococcal infection is short of specificity with diverse clinical manifestations. Cryptococcosis is frequently misdiagnosed at the early stage[5–7]. Lateral flow immunochromatographic assay (LFA) is a rapid diagnostic method for the quantitative or qualitative detection of analytes in complex mixtures providing results within 5–30 min[8].
The diagnosis of cryptococcosis relies on the histopathological examination, pathogenic smear, and culture of pus puncture specimens in the lesion. These methods have the advantage of high specificity, but the sensitivity is low. Moreover, these tests are time-consuming and require auxiliary equipment[9]. LFA can detect samples without special auxiliary equipment, which can also be used for the determination of single samples and preserve the results of the test. As a rapid diagnostic method, LFA has high specificity and sensitivity under the premise of rapid reaction. The USA Food and Drug Administration has approved LFA as a semi-quantitative tool for the rapid detection of cryptococcal capsular polysaccharide antigen in the serum or CSF[10]. Thus, this diagnostic technology has been adopted by most countries for the detection of cryptococcosis. The application of LFA for the rapid diagnosis of cryptococcosis dramatically shortens the time of detection. Furthermore, it provides a basis for the rapid faster diagnosis of cryptococcal disease and guidelines for future treatment. Herein, we collected relevant articles for the meta-analysis to assess LFA for the diagnostic accuracy of cryptococcosis.
Four investigators systematically searched all the articles about the cryptococcus and LFA before July 2019 in the Embase, PubMed, Web of Science, and Cochrane Library databases. We used the keywords “cryptococcus, torula, filobasidiella” and “lateral flow immunochromatographic assay, LFA, colloidal gold immunochromatography: for advanced search. Geographical restrictions were not applied in these articles.
Two investigators systematically screened all of the articles by pre-established screening criteria. The inclusion criteria were as follows: (1) Studies published in English. (2) The purpose of the study was related to LFA and cryptococcosis. (3) Studies limited to original research. (4) Studies related to diagnostics. (5) Data can be extracted to construct fourfold tables. The exclusion criteria were as follows: (1) Duplicate studies, abstracts, conference abstracts, case reports, reviews, editorials. (2) Studies without a reference standard or a detailed number of samples. (3) Samples not from humans. (4) LFA as the reference standard.
In the process of carefully reading the included articles, the investigators simultaneously extracted related data from the studies, including the name of the first author, year of article, study design, geographical distribution of strains, patient population, reference standard, sample type, true positive (TP), false positive (FP), true negative (TN), and false-negative (FN). The process of extracting data is carried out independently by the investigators, and finally, the synthesis results were compared.
We used the Quality Assessment of Diagnostic Accuracy Study (QUADAS–2) guidelines[11] to assess the quality of included studies. Then, we analyzed the risk of bias and applicability concerns by Review Manager 5.0, including patient selection, reference standard, index test, flow, and timing. If the assessment results conflicted, the investigators reviewed the original studies, and a third investigator would intervene to achieve consensus.
We analyzed the extracted data, such as specificity, sensitivity, negative likelihood ratio (NLR), positive likelihood ratio (PLR), and diagnostic odds ratio (DOR), from the included studies using meta-DiSc 1.4 software. Also, we analyzed the summary receiver operating characteristic (SROC) curve and calculated the area under the curve (AUC). According to the sample types, these studies were analyzed by different methods. Due to the lack of adequate data on urine and other samples in the included articles, these samples were analyzed by Review Manager 5.0 software for sensitivity and specificity. Finally, publication bias was evaluated by Stata12.0 software.
A total of 167 publications were retrieved, which decreased to 82 after excluding the duplicates. Also, 18 studies were excluded after screening the abstracts. After full-text review, we included 15 qualified articles[9–10,12–24].
Fifteen studies were published between 2011 and 2019. 13/15 articles reported data from serum samples, seven collected CSF samples, two contained urine samples, and one contained the samples of fingerprick capillary blood and whole venous blood. A total of 9312 samples were included in the meta-analysis, with an average of 620 (range 59–3447) samples. Table 1 summarizes the characteristics of these studies.
We assessed the quality of 15 articles using Review Manager 5.3. (Fig. 1)
We classified the studies into different categories due to the different sample types.
For serum specimens, the merged sensitivity and specificity values were 0.98 (95% CI: 0.96–0.99) and 0.98 (95% CI: 0.97–0.98), respectively. The average PLR of LFA in the serum was 45.05 (95% CI: 26.22–77.40) and the NLR was 0.04 (95% CI: 0.02–0.10). The merged DOR was 1574.65 (95% CI: 730.16–3395.87) and AUC was 0.9766. The results are shown in Figs. 2, 4A.
For CSF specimens, the merged sensitivity and specificity values were 0.99 (95% CI: 0.98–0.99) and 0.99 (95% CI: 0.99 to 0.99), respectively. The average PLR of LFA in CSF was 93.89 (95% CI: 53.54–164.64) and the NLR was 0.03 (95% CI: 0.01–0.07). The merged DOR was 3864.72 (95% CI: 1308.89–11411.28) and AUC was 0.9983. The results are shown in Figs. 3, 4B.
For other samples, the results of sensitivity and specificity are shown in Figure 5.
In this meta-analysis, the data of serum and CSF samples were tested by Stata 12.0 for publication bias. Deek’s funnel plot asymmetry test was used to assess the potential published bias in the included studies. The results of serum and CSF samples indicated that there was no obvious publication bias (Fig. 6).
Cryptococcosis is a disease with a high mortality rate. Therefore, a rapid diagnosis of cryptococcal infection is necessary for patients presenting appropriate clinical symptoms. A comprehensive search with stringent screening criteria retrieved 15 articles eligible for inclusion in the study. These 15 articles encompassed 3901 serum samples, 4403 CSF samples, 1125 urine samples, 1163 venous whole blood samples, and 1163 fingerprick capillary blood samples.
The results in meta-analysis showed that the combined sensitivity of LFA in serum and CSF was 0.98 (0.96–0.99) and 0.99 (0.98–0.99); specificity was 0.98 (0.97–0.98) and 0.99 (0.99–0.99); DOR was 1574.65 (730.16–3395.87) and 2509.29 (184.18–34187.48); SROC AUC was 0.9962 and 0.9983, respectively.
Among these indexes, the PLR of the serum and CSF was >10, while the NLR was <0.1. The SROC AUC of the serum and CSF was close to 1. The SROC curve was close to the upper left corner, which indicated that the area under the curve was large. Both the AUCs were >0.9, indicating that LFA had a relatively high overall diagnostic accuracy for serum and CSF. The DOR of serum and CSF was significant, indicating that the correct diagnosis is far larger than the wrong diagnosis. In conclusion, LFA has a high degree of accuracy in the diagnosis of serum and CSF.
The current analysis of these articles revealed several factors that can explain the observed heterogeneity: the differences in the reference methods in the studies; the same reference standard was not used in the study for identification; the interpretation of the results in LFA and reference methods may cause the artificial error.
Nevertheless, the current study has some limitations. Firstly, we collected all the relevant articles; however, it was difficult to ensure that no publication was missing. Secondly, we only included the articles published in the English language, which may contribute to bias. Thirdly, our study only included the articles from inception to August 2019. The difference in the reference standard might also lead to the heterogeneity of the included studies. Finally, meta-analyses of LFA for the diagnosis of cryptococcosis, only until 2015, were included. Thus, we could comprehensively analyze the accuracy of the LFA diagnosis of the cryptococcal infection.
In summary, our meta-analysis indicated that LFA tested in serum and CSF has high diagnostic accuracy in the diagnosis of cryptococcal infection for high-risk patients, such as HIV-infected patients. LFA performed in urine, or other samples could be a screening tool for the early diagnosis of cryptococcal infection; however, additional studies are required for the substantiation of these results.
Not applicable.
No funding was used to support this study.
All data generated or analyzed during this study are included in this published article and its supplementary information files.
Xu-Guang Guo conceived and designed the experiments. Li-Min Xie, Geng-ling Lin, Hao-Neng Dong and Ying-Xia Liao analyzed the data and made the tables. Li-Min Xie, Ye-Ling Liu and Jian-Feng Qin contributed to the production of figures by the analysis tools. All authors participated in the writing, reading, and revising of the manuscript and approved the final version of the manuscript.
Not applicable.
Not applicable.
The authors declare that they have no competing interests.
AUC: calculated the area under the curve; CSF: cerebrospinal fluid; CM: cryptococcal meningitis; DOR: diagnostic odds ratio; FP: false positive; FN: false negative; HIV: hunman immunodeficiency virus; LFA: Lateral flow immunochromatographic assay; NLR: Negative likelihood ratio; PLR: Positive likelihood ratio; QUADAS: Quality Assessment of Diagnostic Accuracy Study; SROC: Summary receiver operating characteristic; TP: true positive; TN: true negative;
[1]Gibson J F, Johnston S A. Immunity to Cryptococcus neoformansand C.gattii during cryptococcosis[J]. Fungal Genet Biol, 2015, 78:76–86.
[2]Xu J, Eastman A J, Flaczyk A, et al. Disruption of early tumor necrosis dactor alpha signaling prevents classic alactivation of dendritic cells in lung-associated lymph nodes and development of protective immunity against cryptococcal infection [J]. MBio, 2016, 7 (4): e00510–00516.
[3]Brown G D, Denning D W, Levitz S M. Tackling human fungal infections[J]. Sci, 2012, 336(6082):647.
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Table1. Characteristics of the included studies (n = 15).
Reference no. |
First author |
Year |
Geographical distribution of strains |
Study design |
Patient population |
Sample type(s) |
Sample size |
Reference standard |
1 |
Lindsley |
2011 |
Thailand |
prospective |
HIV |
Serum Urine |
538 |
EIA |
2 |
Binnicker |
2012 |
USA |
prospective&retrospective |
SC |
Serum |
634 |
LA |
3 |
McMullan |
2012 |
Australia |
retrospective |
SC |
Serum |
106 |
Comprehensive reference3 |
4 |
Escandón |
2013 |
Colombia |
retrospective |
HIV |
Serum |
421 |
LA |
5 |
Hansen |
2013 |
USA |
prospective |
SC |
Serum CSF |
1000 |
EIA |
6 |
Rugemalila |
2013 |
Tanzania |
prospective |
SC |
Serum |
319 |
LA |
7 |
Boulware |
2014 |
Uganda& South Africa |
prospective&retrospective |
HIV SM |
CSF |
666 |
Culture |
8 |
Lourens |
2014 |
South Africa |
prospective |
HIV SM |
CSF |
465 |
Culture/LA |
9 |
Rivet-Dañon |
2015 |
France |
prospective&retrospective |
IFI1 SC HIV |
Serum CSF |
292 |
LA |
10 |
Suwantarat |
2015 |
America |
retrospective&prospective |
SC |
Serum CSF |
1047 |
EIA/Enhanced reference4 |
11 |
Jitmuang |
2016 |
America |
retrospective |
HIV-N |
Serum CSF |
59 |
LA |
12 |
Cáceres |
2017 |
Colombia |
retrospective |
CIB2 |
Serum CSF |
83 |
LA |
13 |
Frola |
2017 |
Argentina |
prospective |
HIV |
Serum |
123 |
Comprehensive reference5 |
14 |
Temfack |
2018 |
Cameroon |
prospective |
HIV |
Serum |
186 |
EIA |
15 |
Drain |
2019 |
South Africa |
prospective |
HIV |
VWB FCB Urine |
3447 |
EIA/Combined reference6 |
HIV, hunman immunodeficiency virus; SC, suspected cryptococcosis; SM, suspected meningitis; HIV-N,HIV-negative; CSF,cerebrospinal fluid; VWB,venous whole blood; FCB,fingerprick capillary blood; LA, latex agglutination method; EIA, enzyme-linked immunoassay; 1:patients proven or probable invasive fungal infection other than cryptococcosis; 2:patients with or without diagnosis of cryptococcosis were randomly selected from a collection of biological samples stored in the CIB’s biobank; 3:Cryptococcosis was proven if the organism was detected by one or more of culture, histopathology or molecular tests; 4:An enhanced reference method includes data from histopathology, cytopathology, fungal culture, and patient clinical history in addition to EIA results; 5:Pathogen identification of isolates from positive blood cultures was performed using standard microbiology methods (morphological and biochemical tests); 6:A combined reference standard for either a positive CrAg EIA or latex agglutination test.
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