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Research article

Co-expression Network analysis of LncRNA associated with overexpression of DNMT1 in esophageal epithelial cells

Dong Yin, Yi XU, Xu-feng LI, Yan CHEN
DOI: 10.21203/rs.2.12818/v1

Abstract

Screening and preliminary identification of DNMT1 high expression related LncRNA, which is involved in various inter-related signaling pathways, has revealed a theoretical basis for various types of disease mechanisms. LncRNA and mRNA differential expression profiles were identified on a microarray. The 10 LncRNAs with high levels of variation were identified by qRT-PCR. KEGG and GO analyses were used to dissect out differentially expressed mRNAs. Six signal pathways were selected based on the KEGG results of the LncRNA-mRNAs expression network analysis.We found a total of 6987 differentially expressed LncRNAs and 7421 differentially expressed mRNAs were obtained from the microarrays in experimental and control groups (P<0.05; Fold Change >2.0x). GO analysis KEGG pathway analysis showed high expression of DNMT1 in esophageal epithelial cells. Nine pathways involved mRNA up-regulation including natural killer cell mediated cytotoxicity and many other prominent biochemical pathways. Forty six pathways were associated with down regulated mRNAs and ribosomes involving multiple biological pathways. Co-expression network analysis showed that the 8 mRNA and 16 LncRNA were linked to the P53 signaling pathway. Interactions occurred between 22 LncRNA and 11 mRNA in the ErbB signaling pathway, between 19 LncRNA and 8 mRNA in epithelial cell signal transduction of Helicobacter pylori infection. Interactions were present in 19 LncRNAs and 5 mRNAs in the sphingolipid signaling pathway along with 21 with LncRNA and 12 with mRNAs in the PI3K-Akt signaling pathway. Cytotoxicity interactions occurred with 22 LncRNAs and 9 mRNAs in natural killer cells.

Keywords
DNMT1; High expression; LncRNA; Esophageal epithelial cells; Co-expression network analysis

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Preprint: Please note that this article has not completed peer review.
Research article

Co-expression Network analysis of LncRNA associated with overexpression of DNMT1 in esophageal epithelial cells

Dong Yin, Yi XU, Xu-feng LI, Yan CHEN

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Abstract

Screening and preliminary identification of DNMT1 high expression related LncRNA, which is involved in various inter-related signaling pathways, has revealed a theoretical basis for various types of disease mechanisms. LncRNA and mRNA differential expression profiles were identified on a microarray. The 10 LncRNAs with high levels of variation were identified by qRT-PCR. KEGG and GO analyses were used to dissect out differentially expressed mRNAs. Six signal pathways were selected based on the KEGG results of the LncRNA-mRNAs expression network analysis.We found a total of 6987 differentially expressed LncRNAs and 7421 differentially expressed mRNAs were obtained from the microarrays in experimental and control groups (P<0.05; Fold Change >2.0x). GO analysis KEGG pathway analysis showed high expression of DNMT1 in esophageal epithelial cells. Nine pathways involved mRNA up-regulation including natural killer cell mediated cytotoxicity and many other prominent biochemical pathways. Forty six pathways were associated with down regulated mRNAs and ribosomes involving multiple biological pathways. Co-expression network analysis showed that the 8 mRNA and 16 LncRNA were linked to the P53 signaling pathway. Interactions occurred between 22 LncRNA and 11 mRNA in the ErbB signaling pathway, between 19 LncRNA and 8 mRNA in epithelial cell signal transduction of Helicobacter pylori infection. Interactions were present in 19 LncRNAs and 5 mRNAs in the sphingolipid signaling pathway along with 21 with LncRNA and 12 with mRNAs in the PI3K-Akt signaling pathway. Cytotoxicity interactions occurred with 22 LncRNAs and 9 mRNAs in natural killer cells.

Figures

Background

Methods

Results

Discussion

Declarations

References

Tables

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