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Method Article
Detection of iNOS/TNF in cells of the small intestinal lamina propria by FACS
Olga Rojas
Gommerman Lab, University of Toronto
Corresponding Author
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The largest mucosal surface in the body is in the gastrointestinal (GI) tract, a location that is heavily colonized by normally harmless microbes. A key mechanism required for maintaining a homeostatic balance between this microbial burden and the lymphocytes that densely populate the GI tract is the production and trans-epithelial transport of poly-reactive IgA1. Within the mucosal tissues, B cells respond to cytokines, sometimes in the absence of T cell help, undergo class switch recombination (CSR) of their Immunoglobulin (Ig) receptor to IgA, and differentiate to become plasma cells (PC)2. However, IgA-secreting PC likely have additional attributes that are needed for coping with the tremendous bacterial load in the GI tract. Here we describe a detailed method to characterize IgA+B220lowCD11clowiNOS+TNFα+ cells that we named TNFα-iNOS-producing (TiP)-PC in the lamina propria of mice by FACS.
Keywords
Flow Cytometry (FACS), Immunofluorescence Microscopy (IF), Mixed Bone Marrow Chimeras, Small intestinal lamina propria
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Associated Publications
Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut
by Jörg H. Fritz, Olga Lucia Rojas, Nathalie Simard, +12
Nature (16 December, 2011)
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This is a preprint. It has not completed peer review.
Method Article
Detection of iNOS/TNF in cells of the small intestinal lamina propria by FACS
Olga Rojas
Gommerman Lab, University of Toronto
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Dec, 2011
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The largest mucosal surface in the body is in the gastrointestinal (GI) tract, a location that is heavily colonized by normally harmless microbes. A key mechanism required for maintaining a homeostatic balance between this microbial burden and the lymphocytes that densely populate the GI tract is the production and trans-epithelial transport of poly-reactive IgA1. Within the mucosal tissues, B cells respond to cytokines, sometimes in the absence of T cell help, undergo class switch recombination (CSR) of their Immunoglobulin (Ig) receptor to IgA, and differentiate to become plasma cells (PC)2. However, IgA-secreting PC likely have additional attributes that are needed for coping with the tremendous bacterial load in the GI tract. Here we describe a detailed method to characterize IgA+B220lowCD11clowiNOS+TNFα+ cells that we named TNFα-iNOS-producing (TiP)-PC in the lamina propria of mice by FACS.
Figure 1
Associated Publications
Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut
by Jörg H. Fritz, Olga Lucia Rojas, Nathalie Simard, +12
Nature (16 December, 2011)