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Method Article
Florian Muller
Ronald DePinho
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Xenografting of human cancer cell lines injected subcutanously in nude mice has become the standard testing platform for dissecting mechanistic aspects of tumorigenesis and for pre-clinical drug development. However, subcutanous xenografts do not model tumors in their tissue of origin and thus may have some clinically relevant limitations. Xenografted human tumor cells directly injected into their relevant organ may present a more accurate model of human tumors. Here, we describe a rapid protocol for the injection of tumor cell lines into the mouse brain for the establishment of orthotopic tumors.
Keywords
Stereotactic, Orthotopic Xenograft, Mouse Brain
None
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Florian Muller
Ronald DePinho
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Aug, 2012
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Xenografting of human cancer cell lines injected subcutanously in nude mice has become the standard testing platform for dissecting mechanistic aspects of tumorigenesis and for pre-clinical drug development. However, subcutanous xenografts do not model tumors in their tissue of origin and thus may have some clinically relevant limitations. Xenografted human tumor cells directly injected into their relevant organ may present a more accurate model of human tumors. Here, we describe a rapid protocol for the injection of tumor cell lines into the mouse brain for the establishment of orthotopic tumors.
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