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Method Article
Leaf numbering for experiments on long distance signalling in Arabidopsis
Edward Farmer
Farmer Lab
Corresponding Author
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Two of the major features of long distance signals are where they travel and how fast this occurs. The spatial analysis of long distance signals is often necessary prior to temporal analysis. Spatiotemporal analysis lays the foundations for discovering signal propagation mechanisms. This protocol concerns spatial aspects of long distance signalling in Arabidopsis rosettes and uses wounding as the example. However, the principle is applicable to other plants and to other stimuli (pathogenesis, localised high light stress or localised temperature changes etc.). The key to spatial analysis of long distance signalling rests on using uniform populations of plants and giving every relevant organ (e.g. leaf) its own identity prior to analysis. Doing this greatly enhances the reproducibility of experiments and allows researchers to examine questions such: Are source-sink pathways used to conduct long distance signals? or, Are long distance signals bidirectional, travelling from young to old leaves and vice versa ? By carefully numbering each leaf it is possible to investigate signalling within and outside domains formed by interleaf vascular connections. Leaf-to-leaf vascular connections (parastichies) in Arabidopsis rosettes have been delineated 1.
Keywords
Wound, Jasmonic acid, leaf numbering
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Associated Publications
GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling
by Seyed A. R. Mousavi, Adeline Chauvin, François Pascaud, +2
Nature (22 August, 2013)
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This is a preprint. It has not completed peer review.
Method Article
Leaf numbering for experiments on long distance signalling in Arabidopsis
Edward Farmer
Farmer Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Aug, 2013
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant sciences
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Two of the major features of long distance signals are where they travel and how fast this occurs. The spatial analysis of long distance signals is often necessary prior to temporal analysis. Spatiotemporal analysis lays the foundations for discovering signal propagation mechanisms. This protocol concerns spatial aspects of long distance signalling in Arabidopsis rosettes and uses wounding as the example. However, the principle is applicable to other plants and to other stimuli (pathogenesis, localised high light stress or localised temperature changes etc.). The key to spatial analysis of long distance signalling rests on using uniform populations of plants and giving every relevant organ (e.g. leaf) its own identity prior to analysis. Doing this greatly enhances the reproducibility of experiments and allows researchers to examine questions such: Are source-sink pathways used to conduct long distance signals? or, Are long distance signals bidirectional, travelling from young to old leaves and vice versa ? By carefully numbering each leaf it is possible to investigate signalling within and outside domains formed by interleaf vascular connections. Leaf-to-leaf vascular connections (parastichies) in Arabidopsis rosettes have been delineated 1.
Figure 1
Figure 2
Associated Publications
GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling
by Seyed A. R. Mousavi, Adeline Chauvin, François Pascaud, +2
Nature (22 August, 2013)