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Method Article
Multiplex PCR for amplifying mitochondrial and nuclear products in plants
Noemí Colombo
Instituto de Genética, INTA Castelar, Argentina
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
PCR amplification of a single copy nuclear region and a multicopy mitochondrial region together results in the competition between targets unequally represented in a single tube PCR reaction. This protocol allows a correct amplification of both products by adopting a sequential lay out of the PCR reaction which enhances the amplification of the nuclear gene in the first step and the mitochondrial gene in the final step.
Keywords
Multiplex PCR, plant genomes
Figure 1
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Table 1 Master mix components for the 1st step of the multiplex PCR reaction
- supplement0.doc
Table 2 Thermal profile
- supplement0.doc
Table 3 Master mix components for the 2nd step of the multiplex PCR reaction
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This is a preprint. It has not completed peer review.
Method Article
Multiplex PCR for amplifying mitochondrial and nuclear products in plants
Noemí Colombo
Instituto de Genética, INTA Castelar, Argentina
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 31 Dec, 2013
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Genetics
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
PCR amplification of a single copy nuclear region and a multicopy mitochondrial region together results in the competition between targets unequally represented in a single tube PCR reaction. This protocol allows a correct amplification of both products by adopting a sequential lay out of the PCR reaction which enhances the amplification of the nuclear gene in the first step and the mitochondrial gene in the final step.
Figure 1