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Method Article
A protocol for in vivo magnetisation of mice colon and application of physiological mechanical deformations to study mechanotransduction in tumour growth pressure
Maria Elena Fernandez-Sanchez
Institut Curie - Centre de Recherche, CNRS UMR 168 Physicochimie Curie Mechanics and Genetics of Embryonic and Tumour Development PSL Research Univsersity, INSERM, FPGDG
Corresponding Author
Emmanuel Farge
Institut Curie - Centre de Recherche, CNRS UMR 168 Physicochimie Curie Mechanics and Genetics of Embryonic and Tumour Development PSL Research Univsersity, INSERM, FPGDG
Corresponding Author
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This protocol is an innovative method developed to study mechanical pressure in vivo, by subcutaneously inserting a dorsal magnet close to the mouse colon. The implanted magnet generates a magnetic force on ultra-magnetic liposomes (UML), stabilized in the mesenchymal cells of the connective tissue surrounding colonic crypts after intravenous injection. The pressure induced magnetically quantitatively mimics in vivo the endogenous early tumour growth stress in the order of 1200 Pa without affecting tissue stiffness, as monitored by ultrasound strain imaging and shear wave elastography.
Keywords
In vivo tissue magnetisation, mechanical deformations, ultrasound stress measurements
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Method Article
A protocol for in vivo magnetisation of mice colon and application of physiological mechanical deformations to study mechanotransduction in tumour growth pressure
Maria Elena Fernandez-Sanchez
Institut Curie - Centre de Recherche, CNRS UMR 168 Physicochimie Curie Mechanics and Genetics of Embryonic and Tumour Development PSL Research Univsersity, INSERM, FPGDG
Corresponding Author
Emmanuel Farge
Institut Curie - Centre de Recherche, CNRS UMR 168 Physicochimie Curie Mechanics and Genetics of Embryonic and Tumour Development PSL Research Univsersity, INSERM, FPGDG
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Dec, 2014
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol is an innovative method developed to study mechanical pressure in vivo, by subcutaneously inserting a dorsal magnet close to the mouse colon. The implanted magnet generates a magnetic force on ultra-magnetic liposomes (UML), stabilized in the mesenchymal cells of the connective tissue surrounding colonic crypts after intravenous injection. The pressure induced magnetically quantitatively mimics in vivo the endogenous early tumour growth stress in the order of 1200 Pa without affecting tissue stiffness, as monitored by ultrasound strain imaging and shear wave elastography.
Figure 1
Figure 2