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Method Article
RNAseq: mRNA to Illumina library
Marcia Sanders
ORFeome
Corresponding Author
Anne Beall
ORFeome
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol details the RNAseq sample preparation starting with mRNA and ending with quality control of finished libraries. This protocol was used specifically for virus mRNA samples related to ORFeome lab group.
Keywords
RNAseq, TruSeq Stranded mRNA Sample Preparation, Illumina mRNA library
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Supplementary 1 Agilent RNA 6000 Nano Quick Guide Agilent RNA 6000 Nano Kit Quick Start Guide-Rev.C. (2013) Available at: http://www.chem.agilent.com/library/usermanuals/Public/G2938-90037_RNA6000Nano_QSG.pdf.
- supplement0.pdf
Supplementary 3 Agilent High-Sensitivity DNA Quick Guide Agilent High Sensitivity DNA Kit Quick Start Guide-Rev.C. (2013) Available at: http://www.chem.agilent.com/library/usermanuals/Public/G2938-90322_HighSensitivityDNA_QSG.pdf.
- supplement0.pdf
Supplementary 2 Picogreen dsDNA Assay Quant-iT™ PicoGreen ® dsDNA Reagent and Kits-Rev.10-June-2008. (2008) Available at: http://tools.lifetechnologies.com/content/sfs/manuals/mp07581.pdf.
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This is a preprint. It has not completed peer review.
Method Article
RNAseq: mRNA to Illumina library
Marcia Sanders
ORFeome
Corresponding Author
Anne Beall
ORFeome
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Feb, 2015
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol details the RNAseq sample preparation starting with mRNA and ending with quality control of finished libraries. This protocol was used specifically for virus mRNA samples related to ORFeome lab group.