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Method Article
Improved Methods for Cutting, Mounting, and Staining Tissue for Neural Histology
Katherine Kapelsohn
Schmidt Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
When studying brain-behavior relationships, proper histology is crucial in determining how effective the surgical brain manipulation was. If the histology is ruined, for example, there is no way to determine whether or not the behavior observed in a post-op animal is due to lesioning of the intended area, or other factors. Proper staining and mounting of the tissue is necessary to note both the placement and extent of the intended lesion.
After noticing repeated errors in histology when attempting to view final slides under the microscope, it became apparent that a new protocol for histology needed to be developed. The subsequent protocol was developed with the following steps: Slide Subbing, Embedding, Sectioning, Mounting, Staining, and Cover-slipping. Researching current alternative protocols and older methods from the anatomy research community led to many variables to test in determining an ideal protocol. By testing different methods at each step of the process, the following protocol appears to have the best results.
Keywords
Anatomy, Histology, Zebra Finch, Nissl Stain, Staining, Mounting, Embedding, Gelatin, Tissue, Tearing, Brain
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Improved Methods for Cutting, Mounting, and Staining Tissue for Neural Histology Protocol
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This is a preprint. It has not completed peer review.
Method Article
Improved Methods for Cutting, Mounting, and Staining Tissue for Neural Histology
Katherine Kapelsohn
Schmidt Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Mar, 2015
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Neuroscience
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
When studying brain-behavior relationships, proper histology is crucial in determining how effective the surgical brain manipulation was. If the histology is ruined, for example, there is no way to determine whether or not the behavior observed in a post-op animal is due to lesioning of the intended area, or other factors. Proper staining and mounting of the tissue is necessary to note both the placement and extent of the intended lesion.
After noticing repeated errors in histology when attempting to view final slides under the microscope, it became apparent that a new protocol for histology needed to be developed. The subsequent protocol was developed with the following steps: Slide Subbing, Embedding, Sectioning, Mounting, Staining, and Cover-slipping. Researching current alternative protocols and older methods from the anatomy research community led to many variables to test in determining an ideal protocol. By testing different methods at each step of the process, the following protocol appears to have the best results.