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Method Article
CapStarr-seq protocol
Salvatore Spicuglia
Spicuglia's lab (TAGC, Inserm U1090, Aix-Marseille University, Marseille)
Corresponding Author
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Cell-type specific regulation of gene expression requires the activation of promoters by distal genomic elements defined as enhancers. The identification and the characterization of enhancers are challenging in mammals due to their genome complexity. Here we describe the CapStarr-Seq procedure (Vanhille et al. Nat Commun. 2015. doi: 10.1038/ncomms7905), a high-throughput strategy to quantitatively assess enhancer activity in mammals. This approach couples capture of regions of interest to previously developed Starr-seq technique. CapStarr-Seq is applicable to a wide range of biological questions and will provide an efficient tool to better decipher mechanisms involved in gene regulation.
Keywords
Enhancer, high-throughput reporter assay, Starr-seq, capture, mammals
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Table 1 Primer sequences
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Associated Publications
High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq
by Laurent Vanhille, Aurélien Griffon, Muhammad Ahmad Maqbool, +6
Nature Communications (05 August, 2015)
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This is a preprint. It has not completed peer review.
Method Article
CapStarr-seq protocol
Salvatore Spicuglia
Spicuglia's lab (TAGC, Inserm U1090, Aix-Marseille University, Marseille)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 15 Oct, 2015
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Cell-type specific regulation of gene expression requires the activation of promoters by distal genomic elements defined as enhancers. The identification and the characterization of enhancers are challenging in mammals due to their genome complexity. Here we describe the CapStarr-Seq procedure (Vanhille et al. Nat Commun. 2015. doi: 10.1038/ncomms7905), a high-throughput strategy to quantitatively assess enhancer activity in mammals. This approach couples capture of regions of interest to previously developed Starr-seq technique. CapStarr-Seq is applicable to a wide range of biological questions and will provide an efficient tool to better decipher mechanisms involved in gene regulation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Associated Publications
High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq
by Laurent Vanhille, Aurélien Griffon, Muhammad Ahmad Maqbool, +6
Nature Communications (05 August, 2015)