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Method Article
A modified approach to image guided cell based therapy for cardiovascular diseases using cardiac precursor nanoprobe - GloTrack
Lakshmi Kiran Chelluri
Lakshmi Chelluri's Lab,Hyderabad,India
Corresponding Author
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For cell based myocardial repair, a reliable and selective enrichment strategy of the cardiac precursor cells is crucial. Herein, we describe a modified approach which is economical and potent prototype of a cardiac precursor nanoprobe called GloTrack. It functions as a cell label and tracking agent. GloTrack constitutes cardiac specific antibodies - SIRPA and KDR that are bound to the surface of PEG coated SPION, which aids in the segregation of cardiac precursor cells from cardiac/non cardiac origin. We aimed to characterize its physical attributes for size (CLSM, TEM, SEM, Zetasizer), labeling efficiency (FACS), topology (AFM) and surface charge (Zeta potential). In addition, we determined it’s in vivo distribution through MRI. The results establish GloTrack as a negatively charged, 900 nm size on an average with a variable topology, 99% labeling efficiency, having a uniform distribution of SIRPA+/KDR+, and has the feasibility of tracking in vivo using 7T MRI.
Keywords
Cardiac Precursors, Non-invasive MR imaging, SPIONS, Cell based therapy
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Subject Areas
Cell biology
Biochemistry
Biological techniques
Materials science
Nanoscience and technology
Associated Publications
Signal regulatory protein alpha (SIRPA) and kinase domain receptor (KDR) are key expression markers in cardiac specific precursor selection from hADSCs
by Vinod Kumar Verma, Syed Sultan Beevi, Tanya Debnath, +5
Fluorescent magnetic iron oxide nanoparticles for cardiac precursor cell selection from stromal vascular fraction and optimization for magnetic resonance imaging
by Lakshmi Kiran Chelluri, Vinod Kumar Verma, Suguna Ratnakar Kamaraju, +7
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This is a preprint. It has not completed peer review.
Method Article
A modified approach to image guided cell based therapy for cardiovascular diseases using cardiac precursor nanoprobe - GloTrack
Lakshmi Kiran Chelluri
Lakshmi Chelluri's Lab,Hyderabad,India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Feb, 2016
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
For cell based myocardial repair, a reliable and selective enrichment strategy of the cardiac precursor cells is crucial. Herein, we describe a modified approach which is economical and potent prototype of a cardiac precursor nanoprobe called GloTrack. It functions as a cell label and tracking agent. GloTrack constitutes cardiac specific antibodies - SIRPA and KDR that are bound to the surface of PEG coated SPION, which aids in the segregation of cardiac precursor cells from cardiac/non cardiac origin. We aimed to characterize its physical attributes for size (CLSM, TEM, SEM, Zetasizer), labeling efficiency (FACS), topology (AFM) and surface charge (Zeta potential). In addition, we determined it’s in vivo distribution through MRI. The results establish GloTrack as a negatively charged, 900 nm size on an average with a variable topology, 99% labeling efficiency, having a uniform distribution of SIRPA+/KDR+, and has the feasibility of tracking in vivo using 7T MRI.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Associated Publications
Signal regulatory protein alpha (SIRPA) and kinase domain receptor (KDR) are key expression markers in cardiac specific precursor selection from hADSCs
by Vinod Kumar Verma, Syed Sultan Beevi, Tanya Debnath, +5
Fluorescent magnetic iron oxide nanoparticles for cardiac precursor cell selection from stromal vascular fraction and optimization for magnetic resonance imaging
by Lakshmi Kiran Chelluri, Vinod Kumar Verma, Suguna Ratnakar Kamaraju, +7