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Method Article
In vitro microfluidic circulatory system for circulating cancer cells
jiandi wan
Wan's Lab, Rochester Institute of Technology
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
Circulating tumor cells (CTCs) experience hemodynamic shear stress in circulation and play critical roles in cancer metastasis. The effect of shear on CTCs, however, remains less studied. Here, we described a protocol to circulate HCT116 human colon cancer cells in a microfluidic circulatory system mimicking physiologically relevant circulating conditions. This protocol represents a useful scaffold to mimic the transportation of CTCs in circulation and thus provides an effective means to study the effect of shear on CTCs. We anticipate that future studies using the developed system will help us to further investigate the regulatory roles of shear in molecular responses of CTCs.
Keywords
Microfluidics, Circulating tumor cells, Human colon cancer cells, PCR
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- supplement0.pdf
Table 1 PRIMERS FOR REAL-TIME PCR
- supplement0.pdf
Table 1 PRIMERS FOR REAL-TIME PCR
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This is a preprint. It has not completed peer review.
Method Article
In vitro microfluidic circulatory system for circulating cancer cells
jiandi wan
Wan's Lab, Rochester Institute of Technology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Jun, 2016
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Circulating tumor cells (CTCs) experience hemodynamic shear stress in circulation and play critical roles in cancer metastasis. The effect of shear on CTCs, however, remains less studied. Here, we described a protocol to circulate HCT116 human colon cancer cells in a microfluidic circulatory system mimicking physiologically relevant circulating conditions. This protocol represents a useful scaffold to mimic the transportation of CTCs in circulation and thus provides an effective means to study the effect of shear on CTCs. We anticipate that future studies using the developed system will help us to further investigate the regulatory roles of shear in molecular responses of CTCs.