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Method Article
Micro-C XL
Oliver Rando
Rando Lab, UMMS
Corresponding Author
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We present an improved method for analysis of chromosome folding at mononucleosome resolution, Micro-C XL, using long crosslinkers and isolation of insoluble chromatin to greatly increase signal to noise. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally-interacting domains, and higher-order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.
Keywords
Chromosome structure, nuclear architecture, epigenomics
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Formatted protocol Micro-C XL protocol
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This is a preprint. It has not completed peer review.
Method Article
Micro-C XL
Oliver Rando
Rando Lab, UMMS
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 31 Aug, 2016
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We present an improved method for analysis of chromosome folding at mononucleosome resolution, Micro-C XL, using long crosslinkers and isolation of insoluble chromatin to greatly increase signal to noise. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally-interacting domains, and higher-order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.