This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Protocol for DNA extraction from any plant species (alkaline PVPP method)
Ichiro Kasajima
Kasajima
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Unlike model plants such as Arabidopsis and tobacco, DNA extraction from ornamental plants are often difficult, or sometimes nearly impossible. A new method was developed to extract large amount of DNA from such ‘recalcitrant’ ornamental plants. This protocol was actually applicable to any plant species. Researchers just have to prepare healthy leaves of target plant. Leaves do not need to be juvenile leaves on the top of stem. DNA is extracted at similar efficiency from expanded leaves.
Keywords
DNA extraction, plants
Figure 1
Fida hussain
commented on 24 May, 2019
Sir, I want prtocol of plant leaves DNA extract. How leaves DNA extract can you help me
View 1 reply
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Jan, 2017
Community comments: 2
Metrics
Comments: 2
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Protocol for DNA extraction from any plant species (alkaline PVPP method)
Ichiro Kasajima
Kasajima
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Jan, 2017
Community comments: 2
Metrics
Comments: 2
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Unlike model plants such as Arabidopsis and tobacco, DNA extraction from ornamental plants are often difficult, or sometimes nearly impossible. A new method was developed to extract large amount of DNA from such ‘recalcitrant’ ornamental plants. This protocol was actually applicable to any plant species. Researchers just have to prepare healthy leaves of target plant. Leaves do not need to be juvenile leaves on the top of stem. DNA is extracted at similar efficiency from expanded leaves.
Figure 1
Fida hussain
commented on 24 May, 2019
Sir, I want prtocol of plant leaves DNA extract. How leaves DNA extract can you help me
View 1 reply
Ichiro Kasajima
replied on 31 May, 2019
You need 50 mg of healthy leaf in mini-scale extranction, and 1 g in large-scale extraction. Leaf is much better that root or flower for extraction. Most protocols are good for arabidopsis. Most protocols are not good for rose and cyclamen: depends on plant species. 'Alkaline PVPP with 4M NaCl' can extract DNA from most plants. From which plant species do you want to extract DNA?
Ichiro Kasajima
replied on 31 May, 2019
You need 50 mg of healthy leaf in mini-scale extranction, and 1 g in large-scale extraction. Leaf is much better that root or flower for extraction. Most protocols are good for arabidopsis. Most protocols are not good for rose and cyclamen: depends on plant species. 'Alkaline PVPP with 4M NaCl' can extract DNA from most plants. From which plant species do you want to extract DNA?