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Method Article
Efficient Production of Human Hematopoietic Cells from Pluripotent Stem Cells through cAMP Induction
Shobhit Saxena
Molecular Medicine and Gene Therapy, Lund University
Corresponding Author
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Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium in the developing embryo. Mechanisms behind human hematopoietic stem and progenitor cell development remain elusive. Using a human pluripotent stem cell differentiation system, we show that cyclic AMP (cAMP) induction increases HSC-like cell frequencies. Furthermore, in hematopoietic progenitor and stem-like cells, cAMP induction decreased the oxidative stress, created a redox-state balance, and enhanced C-X-C chemokine receptor type 4 (CXCR4) expression, thus benefiting the maintenance of these primitive cells. Our work provides new strategies to upregulate hematopoietic cell generation, and advances the mechanistic understanding of hematopoietic development towards developing transplantable human hematopoietic cells for therapeutic purposes.
Keywords
ES cells, iPSC cells, hematopoietic stem cells, hemogenic endothelium, pluripotency, cell differentiation, flow cytometry, embryoid bodies, cyclic AMP, progenitors, forskolin, oxidative stress, ROS, CD90, CXCR4, CFU assay,
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Associated Publications
Cyclic AMP Signaling through Epac Axis Modulates Human Hemogenic Endothelium and Enhances Hematopoietic Cell Generation
by Shobhit Saxena, Roger E. Rönn, Carolina Guibentif, +2
Stem Cell Reports (21 October, 2016)
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This is a preprint. It has not completed peer review.
Method Article
Efficient Production of Human Hematopoietic Cells from Pluripotent Stem Cells through cAMP Induction
Shobhit Saxena
Molecular Medicine and Gene Therapy, Lund University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Feb, 2017
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium in the developing embryo. Mechanisms behind human hematopoietic stem and progenitor cell development remain elusive. Using a human pluripotent stem cell differentiation system, we show that cyclic AMP (cAMP) induction increases HSC-like cell frequencies. Furthermore, in hematopoietic progenitor and stem-like cells, cAMP induction decreased the oxidative stress, created a redox-state balance, and enhanced C-X-C chemokine receptor type 4 (CXCR4) expression, thus benefiting the maintenance of these primitive cells. Our work provides new strategies to upregulate hematopoietic cell generation, and advances the mechanistic understanding of hematopoietic development towards developing transplantable human hematopoietic cells for therapeutic purposes.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
Cyclic AMP Signaling through Epac Axis Modulates Human Hemogenic Endothelium and Enhances Hematopoietic Cell Generation
by Shobhit Saxena, Roger E. Rönn, Carolina Guibentif, +2
Stem Cell Reports (21 October, 2016)