This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Preparation of monolayer cultures of primary rat and human islet cells on glass for super-resolution and live cell imaging
Steinunn Baekkeskov
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Pancreatic islets of Langerhans consist of interactive micro-societies of different hormone producing endocrine cells, including insulin producing beta cells, glucagon producing alpha cells, somatostatin producing delta cells and pancreatic polypeptide producing PP or gamma cells, which together control glucose homeostasis. High resolution imaging studies of the cell biology of islet cells have been limited by insufficient techniques to culture monolayers of primary pancreatic islet cells on glass coverslips. Combining the approach of using well defined matrices optimized for each species and growth factors required for culture of primary neurons, we describe a simple and reproducible method enabling robust adhesion and formation of monolayers of well connected and fully differentiated human and rodent pancreatic islet endocrine cells. This technical advance enables detailed observation of cellular processes in primary islet cells by live cell and super-resolution microscopy.
Keywords
monolayer cultures, islet cells, live cell imaging, super-resolution imaging
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.docx
Article File Article File
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Apr, 2017
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biological techniques
Associated Publications
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Preparation of monolayer cultures of primary rat and human islet cells on glass for super-resolution and live cell imaging
Steinunn Baekkeskov
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Apr, 2017
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Pancreatic islets of Langerhans consist of interactive micro-societies of different hormone producing endocrine cells, including insulin producing beta cells, glucagon producing alpha cells, somatostatin producing delta cells and pancreatic polypeptide producing PP or gamma cells, which together control glucose homeostasis. High resolution imaging studies of the cell biology of islet cells have been limited by insufficient techniques to culture monolayers of primary pancreatic islet cells on glass coverslips. Combining the approach of using well defined matrices optimized for each species and growth factors required for culture of primary neurons, we describe a simple and reproducible method enabling robust adhesion and formation of monolayers of well connected and fully differentiated human and rodent pancreatic islet endocrine cells. This technical advance enables detailed observation of cellular processes in primary islet cells by live cell and super-resolution microscopy.
Figure 1