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Method Article
Omni-ATAC-seq: Improved ATAC-seq protocol
M. Ryan Corces
Howard Chang Lab
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works universally across multiple cell types and cell contexts with significant improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from material that was previously deemed intractable including frozen tissue samples.
Keywords
ATAC-seq
Figure 1
GUOLIANG LYU
commented on 26 October, 2020
Dear All, many thanks for sharing the method! My questions are: what is the purpose of adding PBS, 1% digitonin, 10% Tween-20 in the Transposition Reaction buffer? to improve the efficiency of the Tn5 transposase? Thank you and best! Guoliang
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Posted 29 Aug, 2017
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Genetics
Associated Publications
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
by M Ryan Corces, Alexandro E Trevino, Emily G Hamilton, +12
Nature Methods (29 August, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Omni-ATAC-seq: Improved ATAC-seq protocol
M. Ryan Corces
Howard Chang Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 29 Aug, 2017
Community comments: 1
Metrics
Comments: 1
PDF Downloads: ...
HTML Views: ...
Subject Areas
Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works universally across multiple cell types and cell contexts with significant improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from material that was previously deemed intractable including frozen tissue samples.
Figure 1
GUOLIANG LYU
commented on 26 October, 2020
Dear All, many thanks for sharing the method! My questions are: what is the purpose of adding PBS, 1% digitonin, 10% Tween-20 in the Transposition Reaction buffer? to improve the efficiency of the Tn5 transposase? Thank you and best! Guoliang
Associated Publications
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
by M Ryan Corces, Alexandro E Trevino, Emily G Hamilton, +12
Nature Methods (29 August, 2017)