Preprint: Please note that this article has not completed peer review.
Method Article

Atomic model building and refinement into high-resolution cryo-EM maps

Bruno Klaholz, S. Kundhavai Natchiar, Alexander Myasnikov, Hanna Kratzat, Isabelle Hazemann
DOI: 10.1038/protex.2017.122

Abstract

The interpretation of high-resolution cryo electron microscopy (cryo-EM) maps relies on the creation of precise atomic models that help analyzing the detailed interactions within a macromolecular complex. In the accompanying paper to this protocol, we describe the high-resolution structure of the human ribosome, which includes numerous chemical modifications. Here we present a generally applicable protocol for atomic model building and refinement into high-resolution cryo-EM maps, which in addition includes the refinement of nucleotides and amino acids with chemical modifications.

Keywords
cryo electron microscopy, atomic model refinement, crystallography, structural biology, human ribosome, ribosome, RNA, RNA modifications

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Preprint: Please note that this article has not completed peer review.
Method Article

Atomic model building and refinement into high-resolution cryo-EM maps

Bruno Klaholz, S. Kundhavai Natchiar, Alexander Myasnikov, Hanna Kratzat, Isabelle Hazemann

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Abstract

The interpretation of high-resolution cryo electron microscopy (cryo-EM) maps relies on the creation of precise atomic models that help analyzing the detailed interactions within a macromolecular complex. In the accompanying paper to this protocol, we describe the high-resolution structure of the human ribosome, which includes numerous chemical modifications. Here we present a generally applicable protocol for atomic model building and refinement into high-resolution cryo-EM maps, which in addition includes the refinement of nucleotides and amino acids with chemical modifications.

Figures

Introduction

Reagents

Procedure

Troubleshooting

References

Acknowledgements

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