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Protocols for evaluating local bacterial viscoelastic environment and mechanical coupling in dilute bacterial suspensions

David Stopar, Simon Sretenovic, Biljana Stojković, Iztok Dogsa, Rok Kostanjšek, Igor Poberaj

Abstract

This manuscript describes a protocol for measuring mechanical coupling of optically trapped bacterial pairs, a protocol for probing microrheological properties of bacterial local environment using optical tweezers, a protocol for visualization of extracellular matrix components, and a protocol for determination of eps gene construct expression. The protocols are used to evaluate mechanical coupling in dilute bacterial suspensions and to probe viscoelasticity of bacterial local environment by microrheology.

Keywords
single cell microbiology, biofilm formation, microrheology, microscopy, optical tweezers

Introduction

Reagents

Equipment

Procedure

Troubleshooting

References

Acknowledgements

Supplementary Files

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Preprint: Please note that this article has not completed peer review.

Protocols for evaluating local bacterial viscoelastic environment and mechanical coupling in dilute bacterial suspensions

David Stopar, Simon Sretenovic, Biljana Stojković, Iztok Dogsa, Rok Kostanjšek, Igor Poberaj

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Abstract

This manuscript describes a protocol for measuring mechanical coupling of optically trapped bacterial pairs, a protocol for probing microrheological properties of bacterial local environment using optical tweezers, a protocol for visualization of extracellular matrix components, and a protocol for determination of eps gene construct expression. The protocols are used to evaluate mechanical coupling in dilute bacterial suspensions and to probe viscoelasticity of bacterial local environment by microrheology.

Introduction

Reagents

Equipment

Procedure

Troubleshooting

References

Acknowledgements

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