Preprint: Please note that this article has not completed peer review.

The selection of single-domain antibody fragments (iDAbs) by intracellular antibody capture in yeast

Terry Rabbitts, Wilawan Bunjobpol
DOI: 10.1038/protex.2018.007

Abstract

The ability to interfere with protein-protein interactions is an objective for basic biological research but also bio-medical research. Intracellular antibody fragments can be developed as tools for study structure-function relationship in normal cells and in disease cells. We present a protocol for screening and improving single domain intracellular antibodies (iDAbs) using yeast cells to provide the intracellular environment.

Keywords
Antibody fragments; iDAbs; intracellular antibodies; PPI; IAC

Figures

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Anticipated Results

References

Acknowledgements

Supplementary Files

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Posted 17 Jan, 2018

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    Preprint: Please note that this article has not completed peer review.

    The selection of single-domain antibody fragments (iDAbs) by intracellular antibody capture in yeast

    Terry Rabbitts, Wilawan Bunjobpol

    STATUS: Posted

    Comments: 0
    PDF Downloads: 0
    HTML Views: 53
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    Abstract

    The ability to interfere with protein-protein interactions is an objective for basic biological research but also bio-medical research. Intracellular antibody fragments can be developed as tools for study structure-function relationship in normal cells and in disease cells. We present a protocol for screening and improving single domain intracellular antibodies (iDAbs) using yeast cells to provide the intracellular environment.

    Figures

    Introduction

    Reagents

    Equipment

    Procedure

    Troubleshooting

    Anticipated Results

    References

    Acknowledgements

    Learn more about our company.