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Method Article
A Hybridization Chain Reaction-based Method for Amplifying Immunosignals
Minmin Luo
National Institute of Biological Sciences (NIBS), Beijing, 102206, China
Corresponding Author
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We describe the development of immunosignal HCR (isHCR), which combines antibody-antigen interactions with Hybridization Chain Reaction technology for amplifying immunofluorescence by up to 2-3 orders of magnitude, and with low background. We further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques. With its highly modular and easily adaptable design, isHCR can be applied broadly in basic research and clinical diagnostics.
Keywords
Immunofluorecence, Neuroscience, Cell Biology, Hybridization Chain Reaction, Multiplexed Labeling
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This is a preprint. It has not completed peer review.
Method Article
A Hybridization Chain Reaction-based Method for Amplifying Immunosignals
Minmin Luo
National Institute of Biological Sciences (NIBS), Beijing, 102206, China
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Jan, 2018
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We describe the development of immunosignal HCR (isHCR), which combines antibody-antigen interactions with Hybridization Chain Reaction technology for amplifying immunofluorescence by up to 2-3 orders of magnitude, and with low background. We further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques. With its highly modular and easily adaptable design, isHCR can be applied broadly in basic research and clinical diagnostics.