Formation of blastoids from mouse embryonic and trophoblast stem cells CURRENT

The blastocyst is the mammalian pre-implantation embryo. It consists of an outer epithelial layer of trophoblast cells surrounding a fluid-filled cavity sheltering the embryonic and primitive endoderm cells. From the mouse blastocyst, trophoblast and embryonic stem cells lines can be derived, which have the capacity to indefinitely self-renew in vitro while maintaining the ability to differentiate into all placental and embryonic lineages, respectively. Here, I describe how to form blastoids (synthetic blastocysts) formed from trophoblast and embryonic stem cells. Blastoids morphologically and transcriptionally resemble E3.5 blastocysts, and can implant in utero. Although not recapitulating the full complexity of the embryo, blastoids are simplified models allowing new approaches in embryology. Contrary to blastocysts, blastoids can be generated in large numbers, which allows to harvest material for in-depth assays (e.g. genomics, biochemistry), run high-throughput screens, and to rapidly perform compartment-specific genetic modifications. As such, blastoids are powerful tools to study the principles of stem cell self-organization and embryonic development. Note: Minor errors in this protocol were corrected on the 18th of May 2018.


Introduction
Early mammalian embryos are notoriously difficult to study due to the limited number of cells and embryos. In addition, it remains difficult to rapidly and efficiently genetically modify sub-populations (e.g. trophoblasts only). This is due to the temporal overlap between lineage commitment and blastocyst morphogenesis, and to the relative speed of blastocyst development that limits the interpretation of compartment-specific inducible models. Finally, forming blastocyst without ICM cells (trophospheres) 1,2 is a tedious process. As such, it has been difficult to study early embryos in depth, for example to assign functional roles to compartment interactions.

Comparison with other methods
Classically, embryos are studied by modulating their development through genetic, epigenetic or diet 3 modifications. The observation of resulting embryos reveals key genes, mechanisms and principles of development. However, embryos are robust, adaptive systems that can compensate for the loss of functions due to, for instance, the redundancy of genes and signalling pathways. Conditional genetic modifications allow modifying sub-populations by targeting specific genes at specific times and within specific sub-populations rather than being deleted from beginning of life. This circumvent, for example, the effect of genes generating early embryonic death. However, it takes a great amount of time and effort to generate conditional knock-out mice. Thus, the function of many genes remains unknown.

Synthetic embryology
Stem cells can recapitulate some aspects of embryonic development in vitro. Aggregates of stem cells that are induced to differentiate in vitro can mimic the gastrulation of Day 6 embryos 3,4 . While it is clear that such models do not capture the full complexity of mammalian embryos, they bring technical advantages: they can be generated in large numbers, which allows to harvest material for in-depth assays (e.g. biochemistry), run high-throughput screens, and allow to rapidly perform compartment-specific genetic modifications. In addition, building synthetic embryos from the bottom up allows to come across unnoticed principles of development. Many pieces of the puzzle must be set up in place for synthetic embryos to develop. For example, the function of Wnt and cAMP on the blastocyst and blastocoel development was not firmly assessed. Blastoids allow to test and generate hypothesis, i.e. that Wnt and cAMP regulate the formation of the blastocoel cavity. It is only when a certain threshold of initial conditions is gathered that synthetic embryos initiate the process of selforganization. Finally, synthetic embryos might form via routes that are slightly different from natural embryos, thus allowing to study in vitro the diversity and redundancy of developmental processes that fuel evolution.
Embryonic Stem Cells (ESCs) 5 and Trophoblast Stem Cells (TSCs) 6,7 lines can be established, which are in vitro analogues of the blastocyst's compartments. Such cell lines can be largely expanded in culture while retaining a blastocyst-like state. Stem cells thus provide a large source of material that can be easily manipulated (e.g., genetically).
Altogether, synthetic embryos offer a new approach to study the genetically-encoded principles of self-organization, and allow for generating novel hypothesis of development. As such this approach is complementing the classical top-down approaches in embryology. Here, I explain how to form blastoids, which morphologically and transcriptionally resemble E3.5 blastocysts and recapitulate key features of trophectoderm development. This model overcomes many limitations of blastocyst research, and proposes novel mechanisms of stem cell self-organization 8 .

Reagents
Culture media:

Fabrication of microwell arrays
The polydimethylsiloxane (PDMS) stamps are formed through replica molding using microstructured silicon wafers. Wafers are processed via deep reactive ion etching as we previously described in several publications 9-11. This fabrication process requires the use of a clean room and special equipment accessible in numerous universities. Once the PDMS stamps are produced, these are used to routinely imprint hydrogel microwell arrays.
Microwells have a diameter and a height of 200 μm. The geometry of the interspace is shown in Figure 1a and allows to extrapolate all dimensions of the microwell array.

Culture of embryonic stem cells.
The ESCs are maintained on a gelatin coating in medium #1 (2i medium) for 11 days. A bank of 50 vials containing 0.5 million cells per vial is prepared. When a blastoid experiment is started, a vial is defrosted and plated on gelatin in medium #1. After two days of culture, the cells are passaged, seeded at 15.000 cells/cm 2 and cultured for two days. In total, ESCs are thus cultured for 15 days in medium #1 before starting the blastoid experiment. This leads to the formation of tightly packed, medium-sized colonies with clear edges (see Figure 1b). Note that different ESCs lines can grow at different speed and that the initial seeding density must be adjusted accordingly to obtain such colonies.

Culture of trophoblast stem cells.
The TSCs are maintained on Matrigel® coatings in medium #3 (TX medium5). The 10 ml bottle of Matrigel® is defrosted by standing overnight in a fridge and aliquoted in 1 ml Eppendorf tubes. Note that both the tubes and the pipet tips must be previously cooled down in a freezer and the aliquoting done on ice to prevent the premature gelification of the Matrigel. The stock of tubes containing Matrigel® is frozen. One tube is defrosted, the day before starting the TSC culture, by standing overnight in a fridge. The tube can then be maintained defrosted in a fridge for several weeks.
Matrigel® is then resuspended 1/90 v/v in ice-cold DMEM/F12. The suspension is added to the cell culture dish (1ml/10cm 2 ) and incubated for one hour inside a lamina flow hood, at room temperature.
The Matrigel® suspension is then removed and the TSCs are seeded in medium #3. TSCs previously culture with serum12 are maintained in medium #3 for 3 weeks before making a bank. A bank of 50 vials containing 0.5 million cells per vial is then prepared. When a blastoid experiment is started, a vial is defrosted and plated on Matrigel® in medium #3. After two days of culture, the cells are passaged, seeded at 15.000 cells/cm 2 and cultured for four days. Note that, similar to ESCs, different TSCs lines can grow at different speed and that the initial seeding density must be adjusted accordingly to obtain colonies as represented in Figure 1b.

Formation of blastoids.
The ESCs are washed with PBS, trypsinized for three minutes to form a single cell suspension. The trypsin is inhibited using the soya bean solution and the cells are carefully suspended in medium #2 (serum medium). Cell concentration is counted using a counting chamber. Cells are then seeded on 8 top of the microwell arrays with a cell concentration resulting in the pooling of a mean of five cells per microwell. One ml of medium #2 is used for the aggregation of the ESCs. Within 24-36 hours, the cells form tight, round aggregates. All cells participate to the formation of the aggregate (see Figure   1b).
After 24-36 hours, the TSCs are then washed with PBS, trypsinized for three minutes to form a single cell suspension. The trypsin is inhibited using the soya bean solution and the cells are carefully suspended in medium #4 (blastoid medium). Cell concentration is counted using a counting chamber.
The medium #2 is largely removed from the microwell arrays and the TSCs are then seeded on top of the microwell arrays containing the ESCs aggregates, with a cell concentration resulting in the pooling of a mean of 12 cells per microwell. Upon settling of the cells within the microwells (15 minutes), one ml of medium #4 is added.
Nota Bene: The difficulty is to synchronize the two cell cultures so that both cultures are ready, in a perfect state, for the experiment. The initial state of the cells (e.g. confluency, morphology of the colonies) is crucial for the success of the experiment.
The time of TSCs seeding is considered as the starting point (0 hours). Within 24 hours, the TSCs aggregate with the ESCs and initiate cavitation. At 24 hours, 1 mM 8Br-cAMP in a 10 ul of F12 medium is added to each microwell array. Within 48 hours, 2-10% of the aggregates formed a blastoid, which expanded and stabilized by 65 hours. A blastoid is defined based on morphological parameters of E3.5 blastocysts, as a cystic structure with an outer circularity superior to 0.9 (circularity = 4pi(area/perimeter 2 ), and a diameter comprised between 70 and 110 micrometers, including a single regular cavity lined by a single layer of TSCs and including ESCs (Figure 1c).
Trophospheres are obtained by seeding TSCs (mean of 12 cells per microwell) on top of microwell arrays that do not contain a ESCs aggregate, in the same medium as used for blastoids (Figure 1c).

Manipulation and dissociation for single cell analysis
Upon formation, blastoids can be harvested using a mouth pipet holding a glass capillary, just like blastocysts. Under a stereomicroscope, specific blastoids can be harvested from individual microwells and pooled into round-bottom 96 well-plates for fixation or dissociation. Transfer the blastocysts to TrypleE 10X (no dilution) and shake for 20 minutes Nota Bene: Upon collagenase treatment, blastoids will stay intact. However, they will collapse after TrypleE treatment. Blastoids / EBs / Trophospheres will gather into one cluster, in the center of the 96 well.
Bring to the stereomicroscope and use a large capillary to dissociate the large cluster, and a small capillary to dissociate into single cells Nota Bene 2: EBs will give a lot more cells than blastoids. Blastoids will give more cells than trophospheres, which are the most difficult to dissociate.
Prepare a 1mL Eppendorf with 200uL of Trypsine inhibitor.
Harvest the cells with a P200 and place in the Eppendorf. Wash each well 3 times with PBS+ and also add in the Eppendorf.
Harvest with a P1000 and push through the filter of a FACS tube Add 30 uL of pre-diluted Hoechst (1/400) Place on ice and FACS as soon as possible.

Timing
From the time of TSCs seeding (t=0), the blastoids form within 65 hours.

Troubleshooting
The total volume of culture media influences the formation of blastoids. For a 12 well-plate format, add 1 ml of medium after the seeding of the cells.
Variations in the concentration of the molecules and in the volume of media will for example influence the formation of the double-layer aggregate. If the double-layers do not form, the aggregates of TSCs and ESCs will grow separately, while attached.

Anticipated Results
Expected results: ESCs aggregate within 24 to 36 hours and should form a tight aggregate in which single cells can hardly be distinguished.
TSCs aggregate and initiate cavitation within 24 hours. The trophectoderm-like structure proliferation and becomes more circular within 65 hours.
A blastoid is defined based on morphological parameters of E3.5 blastocysts, as a cystic structure with an outer circularity superior to 0.9 (circularity = 4 pi (area / perimeter^2), and a diameter comprised between 70 and 110 micrometers, including a single regular cavity lined by a single layer of TSCs and including ESCs.
At 65 hours, 2-10% of the microwells contain a blastoid. 20% of the blastoids contain Gata6+/PDGFRa+ cells at 65 hours. Within these blastoids, there is an average of 3 primitive endoderm-like cells (Gata6+/PDGFRa+). This number is likely to fluctuate between different cell lines.