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Method Article
In vivo combinatorial knockout screens using CRISPR-Cpf1
Sidi Chen
Sidi Chen Laboratory
Corresponding Author
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Genetic interactions have long been challenging to investigate systematically. Recently, CRISPR-Cas9 based approaches have been described that can dissect genetic interactions in a high-throughput manner. However, these existing approaches have important disadvantages that limit their flexibility and scalability, hindering their application to in vivo screens. Here, we develop MCAP (massively-parallel CRISPR-Cpf1 crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. This protocol accompanies Chow et al. Nature Methods, “In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens”.
Keywords
CRISPR, Cpf1, Cas12a, cancer, screen, in vivo, genetic interactions, double knockout, metastasis
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- supplement0.xlsx
Table 1 Readout primer sequences
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This is a preprint. It has not completed peer review.
Method Article
In vivo combinatorial knockout screens using CRISPR-Cpf1
Sidi Chen
Sidi Chen Laboratory
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Apr, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Genetic interactions have long been challenging to investigate systematically. Recently, CRISPR-Cas9 based approaches have been described that can dissect genetic interactions in a high-throughput manner. However, these existing approaches have important disadvantages that limit their flexibility and scalability, hindering their application to in vivo screens. Here, we develop MCAP (massively-parallel CRISPR-Cpf1 crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. This protocol accompanies Chow et al. Nature Methods, “In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens”.
Figure 1
Figure 2