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Research
Triwibowo Ambar Garjito
Institute for Vector and Reservoir Control Research and Development, National Institute of Health Research and Development, MoH Indonesia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7697-9759
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Several methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupae collection, various ovitraps, whole night collection using human landing methods, and larvae collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods
Methods
A mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used: morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.
Results
A total of 44675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2 which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found either between any method and the incidence.
Conclusions
There was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.
Keywords
Aedes aegypti, Aedes albopictus, dengue, collection method, dengue incidence, Indonesia
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Triwibowo Ambar Garjito
Institute for Vector and Reservoir Control Research and Development, National Institute of Health Research and Development, MoH Indonesia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7697-9759
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Frontiers in Medicine.
No community comments so far
No comments provided
Version 1
Posted 04 Nov, 2020
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Frontiers in Medicine.
Background
Several methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupae collection, various ovitraps, whole night collection using human landing methods, and larvae collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods
Methods
A mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used: morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.
Results
A total of 44675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2 which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found either between any method and the incidence.
Conclusions
There was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
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