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Xuemin Wang
University of Missouri-St. Louis and Donald Danforth Plant Science Center
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6251-6745
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Background: Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied.
Results: Here we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing siliques, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis was increased in developing seeds of FAX1- and/or ABCA9-OEs.
Conclusions: These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.
Keywords
Camelina, lipid metabolism, oil production, seed weight, transporters
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CURRENT STATUS: Published
View the published article at Biotechnology for Biofuels and Bioproducts.
No community comments so far
Editorial decision: Accept
On 07 Feb, 2021
Review #1 received
Received 05 Feb, 2021
Reviewer #1 agreed
On 26 Jan, 2021
Reviewers invited
Invitations sent on 25 Jan, 2021
Editor assigned
On 15 Jan, 2021
Submission checks complete
On 15 Jan, 2021
Editor invited
On 15 Jan, 2021
Version 1
Posted 05 Nov, 2020
No comments provided
Editorial decision: Major revision
On 16 Dec, 2020
Review #1 received
Received 19 Nov, 2020
Review #3 received
Received 05 Nov, 2020
Review #2 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 27 Oct, 2020
Reviewer #3 agreed
On 27 Oct, 2020
Reviewers invited
Invitations sent on 26 Oct, 2020
Reviewer #1 agreed
On 26 Oct, 2020
Editor assigned
On 24 Oct, 2020
First submitted
On 23 Oct, 2020
Submission checks complete
On 23 Oct, 2020
Editor invited
On 23 Oct, 2020
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Subject Areas
Biotechnology and Bioengineering
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A new preprint design is coming!
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See the published version of this preprint at Biotechnology for Biofuels and Bioproducts.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Xuemin Wang
University of Missouri-St. Louis and Donald Danforth Plant Science Center
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6251-6745
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Biotechnology for Biofuels and Bioproducts.
No community comments so far
Editorial decision: Accept
On 07 Feb, 2021
Review #1 received
Received 05 Feb, 2021
Reviewer #1 agreed
On 26 Jan, 2021
Reviewers invited
Invitations sent on 25 Jan, 2021
Editor assigned
On 15 Jan, 2021
Submission checks complete
On 15 Jan, 2021
Editor invited
On 15 Jan, 2021
Version 1
Posted 05 Nov, 2020
No comments provided
Editorial decision: Major revision
On 16 Dec, 2020
Review #1 received
Received 19 Nov, 2020
Review #3 received
Received 05 Nov, 2020
Review #2 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 27 Oct, 2020
Reviewer #3 agreed
On 27 Oct, 2020
Reviewers invited
Invitations sent on 26 Oct, 2020
Reviewer #1 agreed
On 26 Oct, 2020
Editor assigned
On 24 Oct, 2020
First submitted
On 23 Oct, 2020
Submission checks complete
On 23 Oct, 2020
Editor invited
On 23 Oct, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Biotechnology for Biofuels and Bioproducts.
Background: Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied.
Results: Here we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing siliques, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis was increased in developing seeds of FAX1- and/or ABCA9-OEs.
Conclusions: These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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