Genetic Characterization of Fall Armyworm in Africa Indicates a Limited Capacity for Transcontinental Population Movements and Evidence of a Second Introduction

The fall armyworm, Spodoptera frugiperda (J.E. Smith) is native to the Americas and a major pest of corn and several other crops of economic importance. The species has characteristics that make it of particular concern as an invasive pest, including broad host range, long-distance migration behavior, and a propensity for eld-evolved pesticide resistance. The discovery of fall armyworm in western Africa in 2016 was followed by what was apparently a remarkably rapid spread throughout sub-Saharan Africa by 2018, causing economic damage estimated in the tens of billions USD and threatening the food security of the continent. Understanding the history of the fall armyworm invasion of Africa and the genetic composition of the African populations is critical to assessing the risk posed to different crop types, the development of effective mitigation strategies, and to make Africa less vulnerable to future invasions of migratory moth pests. This paper tested and expanded on previous studies by combining data from 22 sub-Saharan nations during the period from 2016-2019. The results support initial descriptions of the fall armyworm invasion, including the near absence of the strain that prefers rice, millet, and pasture grasses, while providing additional evidence that the magnitude and extent of FAW natural migration on the continent is more limited than expected. The results also show that a second entry of fall armyworm likely occurred in western Africa from a source different than that of the original introduction. These ndings indicate that western Africa continues to be at high risk of future introductions of FAW, which could complicate mitigation efforts.


Introduction
The noctuid moth Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), commonly called fall armyworm (FAW), is native to the Western Hemisphere. It is the primary insect pest of corn in the southeastern United States, the Caribbean, and South America and is responsible for signi cant economic damage in several other important crops 1 . FAW was detected in western Africa in 2016, which was followed in rapid succession by reports of infestations in most sub-Saharan nations in the subsequent two years [2][3][4][5] .
Perhaps the most broadly assumed explanation for this pattern of detections is that of a single introduction of FAW into western Africa in 2016, followed by rapid migration into southern and eastern Africa 6,7 . This presumed movement of large populations across thousands of kilometers and multiple natural barriers over a short period of time is considered plausible given the behavior of FAW in North America, where FAW undergoes annual migrations from overwintering sites in southern Florida and Texas to infestations as far north as southern Canada, a migratory range approximating 2,500 km traversed over 1-3 months 8, 9 . However, it is important to recognize that this migration occurs over multiple generations in a stepwise fashion, as FAW is a nocturnal ier and so, to the best of our knowledge, is limited to no more than 12 hours of sustained ight 10,11 . These assumptions are consistent with recent laboratory studies using ight mills that showed median ight durations of between 8-10 hours and median ight distances ranging from 21-38 km with a maximum of 70 km per day 12 . Modeling studies further demonstrate that the North American migration behavior is dependent on favorable seasonal wind systems that promote northward migration and agricultural patterns that provide high acreages of preferred host plants along the migratory pathway 10,11 . Whether and to what extent such conditions exist in Africa has not been described.
Alternative explanations for the appearance of rapid dissemination include multiple introductions from the Western Hemisphere or the possibility that the species has long been endemic to Africa but only recently detected. The rst scenario reduces the requirement for extensive trans-continental migration while the second assumes that enhanced surveillance motivated by the initial discovery of FAW in Africa in 2016 led to its subsequent detection across the rest of the continent. Distinguishing between these explanations is important to assessments of risk and projections of economic damage. Single or multiple recent introductions represent worst case scenarios where the invasion of a new pest means there is potential for increased economic impact. Alternatively, if FAW has long been present in Africa, then its "discovery" only provides a new explanation for existing damage.
Our genetic characterizations of the African FAW populations to date are most simply explained by a single introduction into western Africa followed by rapid dispersion into most of the rest of the continent. This conclusion stems from the low genetic variation observed that suggests a recent introduction of a small invasive propagule, and the similarity in the haplotypes found at all locations that is consistent with a common source population [13][14][15][16] . In these studies, genetic variation was compared using segments from two genes, the mitochondrial Cytochrome oxidase subunit I gene (COI) and the Zchromosome-linked gene encoding for the housekeeping enzyme Triosephosphate isomerase (Tpi).
These genetic elements are particularly useful because they carry polymorphisms that are diagnostic for two major FAW groups, historically identi ed as strains, that are morphologically indistinguishable but differ in plant host preference and are, therefore, important considerations when assessing what crops are at risk of FAW infestations [17][18][19] . The C-strain (also call corn-strain) is preferentially found in corn and sorghum, while the R-strain (or rice-strain) predominates in pastures, turf grasses, millet, alfalfa, and rice [19][20][21][22][23] . Single nucleotide polymorphisms (SNPs) in the COI and Tpi genes are typically used to differentiate the two strains in Western Hemisphere populations, with the two markers generally in agreement 18,24,25 .
An unexpected nding from the initial genetic studies of African FAW collections was evidence of population structure, with statistically signi cant differences observed in both COI and Tpi haplotype frequencies between FAW populations in the western African nations of Togo and São Tomé & Príncipe compared to those from central and eastern Africa 16,26 . These results have two important implications. The rst is that persistent geographic differences in haplotype frequencies in collections from similar corn-dominated habitats suggest limitations in natural migration that prevent homogenization of the African FAW populations. This is inconsistent with the hypothesis that natural migration is su cient to explain the rapid trans-continental dispersion of FAW in Africa. The second implication has to do with the identi cation of the two strains. The observed differences in the COI and Tpi haplotype frequencies mean that these two markers show substantial disagreement in their determination of strain identity. As a result, it appears that COI is not an accurate marker of strains in Africa and that a substantial portion of the FAW population there could be derived from interstrain hybridization 14,16,26 . This is of potential signi cance to efforts to control FAW as the behavior of FAW interstrain hybrids has not been extensively characterized.
Understanding the history of the FAW invasion of Africa and how the genetic composition of the African FAW populations changes as it becomes established and adapts to the continent is relevant to developing strategies to limit the introduction of new invasive pests, assess the current threat posed by FAW, and to mitigate the future impact of FAW on African agriculture. To further this effort, the current paper tested and expanded on previous studies by examining collections from additional regions in Africa that included later (2018-2019) time periods. Specimens from a total of 22 sub-Saharan nations comprised of 34 collections from 2016-2019 were analyzed for genetic variations at COI and/or Tpi to test the consistency and stability of the previously described haplotype pro les. The results provide new insight into the magnitude and extent of FAW natural migration on the continent as well as demonstrating that western Africa continues to be at high risk of foreign introductions of FAW. This is of importance to food security in Africa as FAW subpopulations with different host preferences and resistance traits are common in the Western Hemisphere but are not currently present in Africa. Their introduction into the Eastern Hemisphere could signi cantly increase the risk and economic impact of FAW infestations.  DNA from individual specimens were isolated as previously described 27 . In brief, specimens were homogenized in 1.5 ml of phosphate buffered saline (PBS, 20 mM sodium phosphate, 150 mM NaCl, pH 8.0) using a tissue homogenizer (PRO Scienti c Inc., Oxford, CT) or hand-held Dounce homogenizer then pelleted by centrifugation at 6000g for 5 min. at room temperature. The pellet was resuspended in 800 µl Genomic Lysis buffer (Zymo Research, Orange, CA) and incubated at 55°C for 15 min, followed by centrifugation at 10,000 rpm for 5 min. Nucleic acids were puri ed from the supernatant using spincolumn chromatography according to manufacturer's instructions (Zymo Research, Orange, CA).
One microliter of the rst reaction was then ampli ed using primers Tpi412F and Tpi1140R. Relative locations of the primers are described in Figure 2.
Determination of strain-identity using COI and Tpi.
To facilitate the analysis of large numbers of samples, strain identity was initially de ned by a single site in COI or Tpi as previously described 26 , with the identi cation con rmed by comparisons with nearby strain-speci c SNPs (Fig. 2AB). Designation of the COI and Tpi gene sites are preceded by an "m" (mitochondria) or "g" (genomic), respectively. This is followed by the gene name, number of base pairs from the predicted translational start site for COI, or the 5' start of the exon for Tpi and, when relevant, the nucleotide observed. The COIB segment lies in the 3' half of the COI gene. COI-based strain identity was determined by mCOI1164, with an A or G signifying C-strain and a T indicating R-strain ( Fig. 2A). Sites mCOI1176 and mCOI1182 are similarly strain speci c. Strain identity by Tpi is de ned by gTpi183 found in the fourth exon (TpiE4) of the predicted Tpi coding region (Fig. 2B). Site gTpi168 shows the same strain-speci c pattern as gTpi183. Two other sites display hemispheric differences in their association with strain identity due to differences in the R-strain. The nucleotide T is found at gTpi165 (gTpi165T) that correlates with gTpi168C and gTpi183T in Western Hemisphere FAW populations but not in FAW surveyed in Africa 26,28 . Site gTpi180 shows the reciprocal pattern with gTpi180G correlating with R-strain associated gTpi183T in Africa FAW but not with those in the Western Hemisphere.
A complication of using the Tpi marker is that male FAW (Z/Z) carry two copies of the Tpi gene and so can be heterozygous (TpiR/TpiC) for the strain markers. These heterozygous specimens (TpiH) are identi ed by DNA sequencing chromatographs that show overlapping C and T curves at gTpi183, as well as overlapping C/G and C/T curves at gTpi180 and gTpi168, respectively. The number of TpiH specimens were incorporated into the calculations of TpiC and TpiR frequencies. In collections from pheromone traps, all specimens are male and therefore carry two copies of the Tpi gene, with TpiH carrying one copy each of TpiC and TpiR. In these collections the number of the TpiC haplotype was calculated by 2(number of TpiC specimens) + (number of TpiH specimens) and the TpiR haplotype by 2(TpiR) + (TpiH). In the larval collections gender was typically not identi ed. In this case a 1:1 sex ratio was assumed with half the collection considered male. In these collections the number of Tpi haplotypes was calculated as 1.5(TpiC) + (TpiH) or 1.5(TpiR) + (TpiH) for TpiC and TpiR frequencies, respectively.
DNA sequence and statistical analysis.
DNA sequence alignments and comparisons were performed using programs available on the Geneious 10.0.7 software (Biomatters, Auckland, New Zealand). Basic mathematical calculations and generation of graphs were done using Excel and PowerPoint (Microsoft, Redmond, WA). Other statistical analyses including t-tests were performed using GraphPad Prism version 9.1.0 for Mac (GraphPad Software, La Jolla, CA, USA). ANOVA calculations were combined with Tukey multiple comparisons testing to make pair-wise comparisons.

Results
Regional distribution of genetic markers.
Two earlier surveys found evidence for FAW population structure with respect to the frequencies of the mitochondrial COI strain markers and a Tpi exon variant designated TpiCa2 (Fig. 2B)   In the current study the survey was expanded by 24 previously uncharacterized collections from 2016-2019 representing an additional eleven nations. This total of 34 collections were subdivided into two groups, western Africa (21 collections) and eastern Africa (13 collections, Fig. 3), based on the distribution of major agricultural areas that tend to concentrate in regions near the Atlantic and Indian oceans (Fig. 1). A statistically signi cant difference was observed in the COI-CS frequency between the groups (Table 2e). On average a majority of 58% of the FAW tested from the western African collections were COI-CS compared to a mean of 22% in the eastern group (Fig. 1). Multi-year data were available for six countries, Senegal (b), Ghana (c), Togo (d), Benin (e), Tanzania(r), and South Africa (w, Fig. 3). The COI-CS frequency was generally consistent over time at each location with two major exceptions. In Ghana 2016 and Benin 2017, COI-RS was the majority haplotype while COI-CS predominated in the subsequent two years.
With respect to the TpiCa2 haplotype, the previously observed signi cant differences between TogS&P versus other African collections was preserved even with the addition of new eastern collections (Table  2f). When the TogS&P pool was expanded to include other collections from the western region, the mean TpiCa2 frequency was still higher than in the east, 32% compared to 27% (Fig. 4A). However, this difference was no longer statistically signi cant (Table 2g).
Distribution of FAW strains and hybrids.
All the African specimens tested were collected from C-strain preferred host plants or habitats (corn or sorghum), leading to the expectation that the C-strain should be the majority in all collections. However, the COI-CS data suggests a differential distribution of the strains by geography, with the C-strain the majority in western African countries and the R-strain predominating in the more eastern and southern locations. This possibility was tested by examining the distribution of the Tpi strain markers in Africa. The TpiC haplotype, which is diagnostic of the C-strain, predominated in all locations with an overall mean frequency of 87% and a range of 74%-100% (Fig. 4B), with no signi cant difference in the mean TpiC frequency observed between western (91%) and eastern (95%) African FAW populations (Table 2h).
Therefore, unlike the COI markers, the observed TpiC haplotype frequencies agree with expectations from host plants.
A subset of the specimens was analyzed with both COI and Tpi strain markers. Figure 5A describes examples of the crosses both within and between strains that can explain the observed COI and Tpi haplotype combinations. The COI marker is mitochondrial and so is inherited maternally, while the Tpi gene is on the Z-chromosome and displays sex-linked segregation. Figure 5B describes the results from 1197 specimens (404 from eastern Africa, 793 from western Africa) where both COI and Tpi sequence data were available. Two genotypes predominated in Africa. The COI-CS TpiC con guration that is concordant for both C-strain markers is the most common con guration in western Africa with a mean frequency of 50% that is not signi cantly different from the 36% frequency of the discordant COI-RS TpiC group (Fig. 5B, Table 2i). In contrast, the discordant COI-RS TpiC con guration predominates in eastern Africa at a frequency (73%) signi cantly higher than the COI-CS TpiC haplotype (18% , Table 2j). Overall, COI-CS TpiC frequencies in the western Africa group was signi cantly higher than in the eastern group (Table 2k) and, conversely, COI-RS TpiC was signi cantly more frequent in the east than west (Table 2l).
Consistent with previous studies the frequency of TpiR remains very low 14,27 . From a total of 1722 specimens tested only 18 were TpiR. These were found in Benin (3 specimens), the Democratic Republic of Congo (2), Ghana (2), South Africa (2), Senegal (2), and Togo (6), representing less than 5% of the specimens from each location. All 18 TpiR were identical in sequence for the 237 bp TpeI4 segment previously described and deposited into GenBank (MH729873) 14 . Another 211 African specimens produced sequence chromatographs with overlapping curves at certain polymorphic sites that are indicative of a TpiC/TpiR heterozygote (TpiH). The mean TpiH frequency was higher in western Africa (13%) than eastern (8%), but the difference was not statistically signi cant (Table 2m). The TpiH specimens were found in all countries except for Ethiopia, Gabon, and Zimbabwe (where sample sizes were relatively low, <15). This indicates that TpiR is broadly distributed in Africa but is rare and usually found in combination with TpiC as a likely hybrid.

Evidence of a new FAW introduction into western Africa
The COIB segment contains SNPs that subdivide the COI-CS haplotype into four variants, COI-h1-4 ( Fig. 2A), that are found throughout the Western Hemisphere but consistently differ in their relative proportions in a manner that identi es two geographically distinct groups 29,30 . Speci cally, the COI-h4 variant is the predominant form in populations that winter in Florida and most of the Caribbean while COI-h2 is the majority in South America and populations that winter in Texas and Mexico 18,21,29,31 . Using the metric m = (h4-h2)/(h2+h4) we consistently nd that populations from Florida and Puerto Rico give positive values while those from Texas and South America are negative (Fig. 6A) 13,29 . Our past surveys of FAW from Africa found a predominance of COI-h4 in all tested locations and this was generally con rmed by the additional collections in this study. We found that all the collections outside of Ghana and Togo that span most of the African continent were strongly positive, with a mean of +0.97 (Fig. 6A).
Similar results were obtained for collections from multiple corn sites in Ghana in 2016 and 2017 where the COI-h2 haplotype was not found, resulting in an m-value of +1.0 (Fig. 6B). In contrast, October 2018 collections from corn elds in Ejura, Ghana showed a preponderance of the COI-h2 haplotype (25 COI-h2 compared to 12 COI-h4) giving an m-value of -0.35. Ejura is within 50 km of sites sampled in 2016 and 2017 and so these specimens are expected to be part of the same interbreeding population. Collections from Ejura in subsequent months show a return to positive m-values, though at levels below that observed in 2016-7 (Fig. 6B).
A similar pattern was observed in collections from adjacent country of Togo. FAW from multiple locations in Togo (including the inland Vogan zone) in 2016 and from the more coastal Lomé area in 2017 gave positive m-values of +0.96 and +0.98, respectively, with only four specimens expressing the COI-h2 haplotype out of 513 tested (Fig. 6B). But in October 2018, collections from corn in the Vogan zone had an m-value of -0.69, with 11 of the 18 specimens being h2 and four specimens COI-h1, a haplotype that has so far not been found elsewhere in Africa and is a minor haplotype in the Western Hemisphere.
However, subsequent collections from the Vogan zone from November and December 2018 showed highly positive m-values with no COI-h1 or COI-h2 haplotypes detected. Contemporaneous collections from pheromone traps in the neighboring country of Benin showed only positive m-values ranging from +0.73 to +1.00 but with the lowest score occurring in October 2018 (Fig. 6B).

Discussion
The results of this updated survey of the African FAW are in general agreement with past studies and lead to three observations with potentially important implications concerning the entry and migration of FAW within the continent. The rst is that previously observed regional differences in the distribution of the COI-CS haplotype persists even after the inclusion of additional sites and later collections. The second is that the African FAW population is dominated by the C-strain and a lineage that appears to be derived from an interstrain hybridization event, with the R-strain continuing to be mostly absent. The third is evidence of a new incursion of FAW into western Africa that appears to be from a different source than the previous introduction. The potential rami cations of these observations are discussed below.
A map of the distribution of farmland in Africa shows concentrations along the western and eastern coastal nations separated by large covers of natural forest with relatively little agricultural activity ( Fig. 1). This pattern suggests limited availability of preferred host plants for FAW in the central area that could restrict west-east movements of large populations by natural migration, thereby explaining the persistence of the COI-CS asymmetry rst observed in collections from 2016-2017 16 and still detected in this study. However, such limitations in migration run counter to the prevailing invasion scenario of a recent introduction of FAW into western Africa followed by the rapid migration to the rest of sub-Saharan Africa in the next two years. Population movements of that scale occurring on a regular basis should lead to a genetically homogeneous African FAW population. Therefore, the persistence of the observed eastwest difference in COI-CS frequency indicates that transcontinental movements of large numbers of FAW by natural migration is unlikely and suggests that human-assisted migration through trade probably played a signi cant role in the rapid spread of this pest across Africa.
Other moth species also show evidence of a divide between populations in western and eastern Africa. In particular, the noctuid moth Busseola fusca (Fuller) and the pyralid Eldana saccharina (Walker) both show clades de ned by mitochondrial haplotypes that are geographically separated in a manner sharing broad similarity to that displayed by FAW [32][33][34] . Both B. fusca and E. saccharina are native to Africa with the observed segregation attributed to geological and climatic events dating back to the Miocene and Pleistocene eras. The persistence of these phylogeographic patterns to the present day suggests the existence of signi cant physical barriers to natural migration on the African continent that impede homogenization and would be expected to impact the distribution and mixing of FAW populations.
The differential distribution of the COI-CS and COI-RS haplotypes in Africa is particularly interesting because these are commonly used markers for identifying the FAW host strains in Western Hemisphere populations. The strains differ in their association with plant hosts in the eld, with the C-strain preferentially found in corn, sorghum, and cotton while the R-strain preferring turf and pasture grasses, alfalfa, millet, and rice 20,21,35 . The determination of what strains are present is critical to assessments of what crops are at risk of signi cant FAW infestations. However, such assessments are complicated by the fact that the strains are morphologically indistinguishable and so can only be identi ed by a small number of molecular markers that have so far been limited to genetic elements that map to mitochondria (such as COI 17 ) or the sex chromosomes (i.e., Tpi 18 , FR 36 , esterase 19 ). The association of the COI and Tpi strain markers with FAW collected from different plant host species has been consistently observed in surveys from both Americas, indicating that the strains are broadly distributed and a general characteristic of the species 22,23,37 . However, this correspondence is not absolute. For example, typically about 20% of FAW collected from corn hosts in the Americas display R-strain markers, and there are multiple FAW collections from corn or rice host plants where a majority will display the opposing strain markers 21,24,38 . These observations suggest that the association between FAW strain and plant host is more of a preference than a requirement, consistent with laboratory feeding studies indicating that both strains can successfully develop on the same set of plant hosts 39,40 . In addition, while reproductive barriers between the strains have been observed, they are incomplete, with successful hybridization between strains demonstrated in the laboratory and evidence of signi cant hybrid frequency found in eld populations 19,41−44 . The hybrids appear to differ from the parental strains with respect to mating behavior and reproductive compatibility [41][42][43] , but the impact on plant host preference remains uncertain.
Because gene ow between strains is directly dependent on the formation of interstrain hybrids, we expect that the amount of strain divergence at any location will be impacted by whether and to what degree differential plant host preferences deter mating between strains. If this factor is signi cant, then divergence should tend to increase in locations where the primary hosts for each strain are abundant and separated, as under these conditions the strains can remain segregated. In contrast, in habitats with less host variety or abundance the two strains are more likely to overlap out of necessity, increasing the likelihood of cross hybridization. Given these considerations, it is likely that FAW displays a complex population structure made up of the C-strain, the R-strain, and inter-strain hybrids, where the proportion of each group and the frequency of mating between and within groups will depend upon the types and distributions of local plant hosts. If correct, then the degree of divergence between the two strains as measured by genetic differentiation could vary signi cantly by location. This scenario could explain the contradictory results from recent studies where differences between the strains at the whole genome level were detected in some comparisons 45 , but not in others 46 .
The situation in Africa differs from the Americas in that while both COI-CS and COI-RS are detected at high frequency, the TpiR haplotype is very rare in Africa, present in less than 1% of all specimens even in collections from habitats dominated by R-strain preferred plant hosts 27 . The Africa population is dominated by two COI Tpi con gurations, COI-CS TpiC present in 41% of specimens and COI-RS TpiC at 47%, with the former preferentially found in the western Africa grouping of collection sites and the latter in eastern Africa (Fig. 3). The COI-CS TpiC con guration is normally representative of the C-strain and is the predominant haplotype found in specimens from corn elds in the Western Hemisphere 16, 21,22 . The COI-RS TpiC is a con guration predicted to arise from an interstrain cross between a R-strain female and C-strain male with the daughters then back crossed to C-strain males (RC hybrid, Fig. 3). This con guration is found at variable frequencies in the Western Hemisphere, with the highest frequencies associated with C-strain preferred host plants 18,24 . These observations suggest that the COI-RS TpiC population is behaving as the C-strain with respect to plant host use and this seems to be the case in Africa as reports of agricultural damage by FAW has consistently been limited to C-strain plants like corn and sorghum 7,27 .
Given these results, we believe that the COI-RS TpiC population should be considered part of the C-strain group despite its derivation from an interstrain hybrid mating. Justi cation for this assumption comes from consideration of the effectiveness of Z-linked markers such as Tpi to distinguish strains, which can best be explained if the primary determinants for strain identity are also on the Z-chromosome and therefore physically linked to the Tpi gene. This proposition is consistent with observations that genetic differences between lepidopteran species are disproportionately sex-linked 47 . Based on this reasoning, it is likely that strain identity is de ned primarily, if not solely, by the Z-chromosome, and we note that the TpiC marker indicates that the Z-chromosome of both COI-CS TpiC and COI-RS TpiC is of the C-strain. If these assumptions are correct, then we anticipate no signi cant differences in the behaviors of the COI-CS TpiC and COI-RS TpiC groups and suggest that their differential distribution across western and eastern Africa is probably due to chance.
The evidence of a second FAW introduction into Africa comes from what appears to be an in ux of the TX-type COI-h2 haplotype in 2018 into Ghana, Togo, and perhaps Benin (Fig. 6B) and Benin from other years, the pooled COI-h2 frequency was only 0.5% (9/1501) with a range from 0.00-0.02%. Once introduced, the COI-h2 haplotype would be expected to disperse into the much larger COI-h4 population and become increasingly di cult to detect. This appears to be what occurred as the COI-h4 haplotype again predominated in Ghana and Togo after October 2018 (Fig. 5B).
If this incidence of COI-h2 does represent a new incursion it appears to be from a different source than that of the original introduction that gave rise to the predominantly FL-type COI-h4 composition of the Africa population. The possibility of a second incursion of Western Hemisphere FAW into western Africa is troubling as it suggests that the conditions that allowed for the rst introduction are still in place despite efforts to improve monitoring and food security. This means that FAW subpopulations of concern thought to be currently rare or absent in Africa could be introduced at any time. This includes the R-strain, which would put important crops like rice, millet, and forage grasses at risk, and lines resistant to the Cry1F Bt-pesticide that have compromised certain Bt-products in Puerto Rico 48 .
In summary, the results for this genetic survey of FAW in Africa demonstrate the value of continued surveillance of pest populations at the continental level. FAW is in the process of becoming established in Africa with the distribution of permanent populations and pattern of regional migrations still to be determined. Identi cation of genetic structure as found for the COI-CS haplotype can de ne the magnitude and limits of natural migration. Evidence of a second incursion of FAW, most likely from the Western Hemisphere, indicate that continued introductions are plausible, which could rapidly alter the composition of the Africa population with respect to pesticide resistance and host range. Figure 1 Map of croplands in sub-Saharan Africa with locations of collection sites that are detailed in Table 1   Bar graph describing COI haplotype frequencies in western and eastern Africa. Collections are as described in Table 1. The mean ± the Standard Error of the Mean (SEM) for different regions is presented above the graph. Asterisks indicate data from earlier studies.

Figure 4
Bar graphs indicating frequency of Tpi haplotypes in western and eastern Africa. Collections are as described in Table 1. The mean ± SEM for different regions is presented above the graph. Asterisks indicate data from earlier studies. A, frequency of the TpiCa2 haplotype. B, frequency of the TpiC haplotype diagnostic of the C-strain.

Figure 5
Description of origins and frequencies of the different observed COI Tpi haplotype con gurations. A, describes crosses that can produce different COI Tpi combinations. The COI haplotype is maternally inherited and indicated by either a circle (female) or square (male), with shading differentiating COI-CS from COI-RS. The Z-linked Tpi gene is present in one copy in females (Z/W) and two copies in males (Z/Z) and undergoes sex-linked segregation. "CR hybrid" indicates cross between a C-strain female and Rstrain male, while "RC hybrid" de nes the reciprocal mating. B, describes the frequencies of the different COI Tpi con gurations in western and eastern Africa.