See the published version of this preprint at Plant Methods.
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Methodology
Daniel J Robertson
University of Idaho
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1089-0249
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Measurements of rind and culm thickness and stem radius / diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measurements of rind thickness and diameter has been developed. The suitability of the new method for implementation in plant studies is presented.
Results: The novel rind puncture technique was used to obtain measurements of rind thickness and diameter for samples of Poison Hemlock (Conium maculatum). The rind puncture measurements were strongly correlated with caliper measurements (R2 > 0.97) and photographic image analysis measurements (R2 > 0.84). The capacity for high throughput measurements using the rind puncture technique was determined to exceed that of caliper measurements and image analysis techniques.
Conclusions: The rind puncture technique shows promise as a high throughput method for determining rind thickness and diameter as it is cost effective and non-lethal. The authors are currently working to develop a custom handheld apparatus to allow the novel rind puncture method to be used in field work. High throughput field-based measurements of rind thickness and diameter are needed to help address the problem of stalk lodging (failure of grain crops to remain upright until harvest).
Keywords
Rind, Thickness, Diameter, Penetration, Puncture, Stalk, Internode, Plant, High throughput, Phenotype
Figure 1
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Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- 011.csv
- 014.csv.png
- 013.csv.png
- 014.csv
- 021.csv
- 012.csv.png
- 013.csv
- 012.csv
- 011.csv.png
- 023.csv.png
- 031.csv
- 024.csv
- 022.csv.png
- 023.csv
- 021.csv.png
- 022.csv
- 026.csv.png
- 026.csv
- 032.csv
- 024.csv.png
- 025.csv.png
- RindPunctureInstronMethod2019.imccyclic
- 025.csv
- 034.csv
- 033.csv
- 032.csv.png
- 031.csv.png
- 033.csv.png
- 3.jpg
- ExplanationofSupplementaryData.docx
- 034.csv.png
- RindPunctureAnalysisAlgorithim.m
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Plant Methods.
Version 2
Posted 11 Mar, 2020
No community comments so far
Editorial decision: Accept
On 23 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 12 Mar, 2020
Reviewers invited
Invitations sent on 11 Mar, 2020
Reviewer #1 agreed
On 11 Mar, 2020
Reviewer #2 agreed
On 11 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 21 Feb, 2020
Review #2 received
Received 19 Feb, 2020
Reviewer #2 agreed
On 10 Feb, 2020
Review #1 received
Received 22 Jan, 2020
Reviewer #1 agreed
On 07 Jan, 2020
Reviewers invited
Invitations sent on 06 Jan, 2020
Editor assigned
On 29 Dec, 2019
Submission checks complete
On 28 Dec, 2019
Editor invited
On 28 Dec, 2019
First submitted
On 27 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Plant Methods.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Methodology
Daniel J Robertson
University of Idaho
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1089-0249
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Plant Methods.
Version 2
Posted 11 Mar, 2020
No community comments so far
Editorial decision: Accept
On 23 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 12 Mar, 2020
Reviewers invited
Invitations sent on 11 Mar, 2020
Reviewer #1 agreed
On 11 Mar, 2020
Reviewer #2 agreed
On 11 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 21 Feb, 2020
Review #2 received
Received 19 Feb, 2020
Reviewer #2 agreed
On 10 Feb, 2020
Review #1 received
Received 22 Jan, 2020
Reviewer #1 agreed
On 07 Jan, 2020
Reviewers invited
Invitations sent on 06 Jan, 2020
Editor assigned
On 29 Dec, 2019
Submission checks complete
On 28 Dec, 2019
Editor invited
On 28 Dec, 2019
First submitted
On 27 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Plant Methods.
Background: Measurements of rind and culm thickness and stem radius / diameter are important to biomechanical, ecological and physiological plant studies. However, many methods of measuring rind thickness and diameter are labor intensive and induce plant fatality. A novel rind puncture methodology for obtaining measurements of rind thickness and diameter has been developed. The suitability of the new method for implementation in plant studies is presented.
Results: The novel rind puncture technique was used to obtain measurements of rind thickness and diameter for samples of Poison Hemlock (Conium maculatum). The rind puncture measurements were strongly correlated with caliper measurements (R2 > 0.97) and photographic image analysis measurements (R2 > 0.84). The capacity for high throughput measurements using the rind puncture technique was determined to exceed that of caliper measurements and image analysis techniques.
Conclusions: The rind puncture technique shows promise as a high throughput method for determining rind thickness and diameter as it is cost effective and non-lethal. The authors are currently working to develop a custom handheld apparatus to allow the novel rind puncture method to be used in field work. High throughput field-based measurements of rind thickness and diameter are needed to help address the problem of stalk lodging (failure of grain crops to remain upright until harvest).
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- 011.csv
- 014.csv.png
- 013.csv.png
- 014.csv
- 021.csv
- 012.csv.png
- 013.csv
- 012.csv
- 011.csv.png
- 023.csv.png
- 031.csv
- 024.csv
- 022.csv.png
- 023.csv
- 021.csv.png
- 022.csv
- 026.csv.png
- 026.csv
- 032.csv
- 024.csv.png
- 025.csv.png
- RindPunctureInstronMethod2019.imccyclic
- 025.csv
- 034.csv
- 033.csv
- 032.csv.png
- 031.csv.png
- 033.csv.png
- 3.jpg
- ExplanationofSupplementaryData.docx
- 034.csv.png
- RindPunctureAnalysisAlgorithim.m
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