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Background
Non-alcoholic fatty liver disease (NAFLD) is viewed as the hepatic manifestation of metabolic syndrome. Methionine metabolites have been linked to metabolic syndrome and its related diseases. Whether methionine metabolites levels are associated with NAFLD remains unclear. The study aimed to assess the association between methionine metabolites and NAFLD.
Methods
This cross-sectional study included a total of 2814 individuals aged 40–75 years old. All participants underwent anthropometric measurements, laboratory tests and abdominal ultrasonography. Multivariable logistic regression analysis was performed to estimate the association of methionine metabolites with NAFLD.
Results
Overall, 1446 with and 1368 without NAFLD were enrolled in this study. Participants with NAFLD had significantly higher serum S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and homocysteine (Hcy) levels, and a lower S-adenosylmethionine/S-adenosylhomocysteine (SAM/SAH) ratio than those without NAFLD (all P < 0.001). After adjusting multiple confounders, odds ratios (ORs) (95% confidence interval [CI]) for quartile 4 vs quartile 1 of SAH, Hcy and SAM/SAH ratio were 1.65 (1.27–2.14), 1.63 (1.26–2.12) and 0.63 (0.49–0.83), respectively (all P for trend < 0.01). In addition, serum SAH, Hcy levels and SAM/SAH ratio were significantly correlated with degree of hepatic steatosis (all P for trend < 0.001).
Conclusion
Elevated serum SAH, Hcy levels and lower SAM/SAH ratio were independently associated with the presence of NAFLD in middle-aged and elder Chinese.
Keywords
Methionine metabolites, Non-alcoholic fatty liver disease, Hepatic steatosis, S-adenosylmethionine, S-adenosylhomocysteine, homocysteine
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- FigS1NM.tif
Methionine metabolism. SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; Hcy, homocysteine; ATP, adenosine triphosphate; MAT, methionine adenosyltransferase; MS, methionine synthase; SAHH, S-adenosylhomocysteine hydrolase; MT, methyltransferase.
- TableS1.docx
Analytical performance and parameters of the detection of serum methionine metabolites by UHPLC/MS-MS.
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CURRENT STATUS: Under Review
Version 1
Posted 09 Nov, 2021
No community comments so far
Reviews received
Received 28 Nov, 2021
Reviewers invited
Invitations sent on 07 Nov, 2021
Editor assigned
On 05 Nov, 2021
Submission checks complete
On 04 Nov, 2021
Editor invited
On 04 Nov, 2021
First submitted
On 03 Nov, 2021
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This preprint is under consideration at Nutrition & Metabolism. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 09 Nov, 2021
No community comments so far
Reviews received
Received 28 Nov, 2021
Reviewers invited
Invitations sent on 07 Nov, 2021
Editor assigned
On 05 Nov, 2021
Submission checks complete
On 04 Nov, 2021
Editor invited
On 04 Nov, 2021
First submitted
On 03 Nov, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Non-alcoholic fatty liver disease (NAFLD) is viewed as the hepatic manifestation of metabolic syndrome. Methionine metabolites have been linked to metabolic syndrome and its related diseases. Whether methionine metabolites levels are associated with NAFLD remains unclear. The study aimed to assess the association between methionine metabolites and NAFLD.
Methods
This cross-sectional study included a total of 2814 individuals aged 40–75 years old. All participants underwent anthropometric measurements, laboratory tests and abdominal ultrasonography. Multivariable logistic regression analysis was performed to estimate the association of methionine metabolites with NAFLD.
Results
Overall, 1446 with and 1368 without NAFLD were enrolled in this study. Participants with NAFLD had significantly higher serum S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and homocysteine (Hcy) levels, and a lower S-adenosylmethionine/S-adenosylhomocysteine (SAM/SAH) ratio than those without NAFLD (all P < 0.001). After adjusting multiple confounders, odds ratios (ORs) (95% confidence interval [CI]) for quartile 4 vs quartile 1 of SAH, Hcy and SAM/SAH ratio were 1.65 (1.27–2.14), 1.63 (1.26–2.12) and 0.63 (0.49–0.83), respectively (all P for trend < 0.01). In addition, serum SAH, Hcy levels and SAM/SAH ratio were significantly correlated with degree of hepatic steatosis (all P for trend < 0.001).
Conclusion
Elevated serum SAH, Hcy levels and lower SAM/SAH ratio were independently associated with the presence of NAFLD in middle-aged and elder Chinese.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- FigS1NM.tif
Methionine metabolism. SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; Hcy, homocysteine; ATP, adenosine triphosphate; MAT, methionine adenosyltransferase; MS, methionine synthase; SAHH, S-adenosylhomocysteine hydrolase; MT, methyltransferase.
- TableS1.docx
Analytical performance and parameters of the detection of serum methionine metabolites by UHPLC/MS-MS.
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