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Short Report
Masayo Kushiro
National Agriculture and Food Research Organization
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8876-0316
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A rapid HPLC-UV method was developed for the quantitative analysis of kojic acid contained in fungal agar media. The methanol extract from fungal agar media was diluted with acetic acid aqueous solution and injected into HPLC. The separation was achieved on a C18 column within 10 minutes in an isocratic mode. The mobile phase was composed of water, methanol, and acetic acid. Spike and recovery tests were done using three representative agar media, and accuracy, precision and limit of detection were preferable. The developed method was applicable for the analysis of kojic acid in two Aspergillus strains practically, showing the usability of this method to evaluate the fungal ability to produce a beneficial secondary metabolite, kojic acid.
Keywords
Aspergillus, secondary metabolite, kojic acid, HPLC, spectrometry
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- 211102Table12.xlsx
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This preprint is under consideration at Mycopathologia. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Short Report
Masayo Kushiro
National Agriculture and Food Research Organization
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8876-0316
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 08 Nov, 2021
No community comments so far
Reviews received
Received 08 Nov, 2021
Reviewers invited
Invitations sent on 05 Nov, 2021
Editor assigned
On 05 Nov, 2021
First submitted
On 03 Nov, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A rapid HPLC-UV method was developed for the quantitative analysis of kojic acid contained in fungal agar media. The methanol extract from fungal agar media was diluted with acetic acid aqueous solution and injected into HPLC. The separation was achieved on a C18 column within 10 minutes in an isocratic mode. The mobile phase was composed of water, methanol, and acetic acid. Spike and recovery tests were done using three representative agar media, and accuracy, precision and limit of detection were preferable. The developed method was applicable for the analysis of kojic acid in two Aspergillus strains practically, showing the usability of this method to evaluate the fungal ability to produce a beneficial secondary metabolite, kojic acid.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- 211102Table12.xlsx
Table 1-2
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