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Research article
xujun zhu
College of Horticulture, Nanjing Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8932-4324
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: γ-Aminobutyric acid (GABA), a signal molecule, is regarded as the intersection of carbon and nitrogen metabolism, but its contributions to flavonoid metabolism in tea plants during the whole growth cycle remain unclear, and the correlation between the GABA shunt and flavonoid metabolism in tea plants is worth exploring. Secondary metabolites and their correlations with the taste qualities of tea plants (Camellia sinensis) during different seasons have been investigated.
Results: Related secondary metabolites and transcript profiles of genes encoding enzymes in the GABA shunt, flavonoid pathway and polyamine biosynthesis were measured throughout the whole tea plant growth season and after exogenous GABA applications. In addition, levels of differentially expressed proteins were measured after treatment with or without exogenous GABA. The tea leaves showed the highest metabolite concentrations in spring. CsGAD, CsGABAT, CsSPMS, CsODC, CsF3H and CsCHS were found to be important genes in the GABA and anthocyanin network.
Conclusion: GABA and anthocyanin concentrations showed a positive correlation, to some extent, and CsF3H and CsCHS played important roles in the GABA and anthocyanin network. Further studies should focus on exploring GABA and flavonoid metabolism through the transgenic engineering of tea plants.
Keywords
Growth time, Differentially expressed proteins, GABA shunt, Flavonoid metabolism, Anthocyanins
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- supplyfig.1.pdf
Fig. S1. The correlation co-efficiencies of gene expression levels between flavonoid metabolism and the GABA shunt
- supplytable1.docx
Table S1. The primer pairs information used in this study.
- supplytable2.docx
Table S2. The correlation co-efficiencies between chemicals in flavonoid metabolism, chlorophyll content and chemicals related to GABA shunt (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable3.docx
Table S3. The correlation co-efficiencies between gene expression levels and chemicals in flavonoid metabolism and chemicals related to the GABA shunt (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable4.docx
Table S4. The correlation co-efficiencies between gene expression levels related to the GABA shunt and the contents of anthocyanin, GABA, putrescine, spermine and spermidine (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable5.doc
Table S5. The concentrations of free amino acids in tea leaves over the whole plant growth season
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
xujun zhu
College of Horticulture, Nanjing Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8932-4324
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Nov, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: γ-Aminobutyric acid (GABA), a signal molecule, is regarded as the intersection of carbon and nitrogen metabolism, but its contributions to flavonoid metabolism in tea plants during the whole growth cycle remain unclear, and the correlation between the GABA shunt and flavonoid metabolism in tea plants is worth exploring. Secondary metabolites and their correlations with the taste qualities of tea plants (Camellia sinensis) during different seasons have been investigated.
Results: Related secondary metabolites and transcript profiles of genes encoding enzymes in the GABA shunt, flavonoid pathway and polyamine biosynthesis were measured throughout the whole tea plant growth season and after exogenous GABA applications. In addition, levels of differentially expressed proteins were measured after treatment with or without exogenous GABA. The tea leaves showed the highest metabolite concentrations in spring. CsGAD, CsGABAT, CsSPMS, CsODC, CsF3H and CsCHS were found to be important genes in the GABA and anthocyanin network.
Conclusion: GABA and anthocyanin concentrations showed a positive correlation, to some extent, and CsF3H and CsCHS played important roles in the GABA and anthocyanin network. Further studies should focus on exploring GABA and flavonoid metabolism through the transgenic engineering of tea plants.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- supplyfig.1.pdf
Fig. S1. The correlation co-efficiencies of gene expression levels between flavonoid metabolism and the GABA shunt
- supplytable1.docx
Table S1. The primer pairs information used in this study.
- supplytable2.docx
Table S2. The correlation co-efficiencies between chemicals in flavonoid metabolism, chlorophyll content and chemicals related to GABA shunt (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable3.docx
Table S3. The correlation co-efficiencies between gene expression levels and chemicals in flavonoid metabolism and chemicals related to the GABA shunt (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable4.docx
Table S4. The correlation co-efficiencies between gene expression levels related to the GABA shunt and the contents of anthocyanin, GABA, putrescine, spermine and spermidine (* and ** represent p < 0.05 and p < 0.01, respectively)
- supplytable5.doc
Table S5. The concentrations of free amino acids in tea leaves over the whole plant growth season
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