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Research
Shuying Liu
Inner Mongolia Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5502-9578
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Endogenous retroviruses (ERVs) exert important biological roles, such as mammalian placental development and suppression of the infection of exogenous retrovirus. In addition, ERVs could also inhibit the proliferation of cell. The envelope-protein (Env) of endogenous Jaagsiekte sheep retroviruses (enJSRVs) possesses fusogenic activity, which promoted the formation of nonproliferative multinucleated syncytiotrophoblasts.
Methods
The proliferation of HeLa cells was detected by MTT. Six samples were extracted for RNA-seq transcriptome analysis. Quantitative real-time PCR (qRT-PCR) and western blotting were employed for the validation of interested target.
Results
enJSRV-Env transfection inhibited the proliferation ability of Hela cells and 170 differentially expressed genes (DEGs) were obtained by RNA-seq analysis. Among these, 5 DEGs (BHLHE41, CCN1, DLX2, DUSP6 and SH2D5) were validated by qRT-PCR, which closely related with proliferation. Western blotting analysis showed that the expression of DUSP6 and p-ERK1/2 was decreased, which suggested that ERK1/2 signaling pathway may be involved in enJSRV-Env transfection.
Conclusions
enJSRV-Env transfection inhibited the proliferation of Hela cells, probably via DUSP6 and ERK1/2 signaling pathway.
Keywords
enJSRV-Env, Hela cells, RNA-seq
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CURRENT STATUS: Under Review
Version 1
Posted 22 Nov, 2021
No community comments so far
Reviewers invited
Invitations sent on 27 Nov, 2021
Editor assigned
On 26 Nov, 2021
Submission checks complete
On 19 Nov, 2021
First submitted
On 04 Nov, 2021
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Subject Areas
Virology
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This preprint is under consideration at Virology Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Shuying Liu
Inner Mongolia Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5502-9578
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Nov, 2021
No community comments so far
Reviewers invited
Invitations sent on 27 Nov, 2021
Editor assigned
On 26 Nov, 2021
Submission checks complete
On 19 Nov, 2021
First submitted
On 04 Nov, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Endogenous retroviruses (ERVs) exert important biological roles, such as mammalian placental development and suppression of the infection of exogenous retrovirus. In addition, ERVs could also inhibit the proliferation of cell. The envelope-protein (Env) of endogenous Jaagsiekte sheep retroviruses (enJSRVs) possesses fusogenic activity, which promoted the formation of nonproliferative multinucleated syncytiotrophoblasts.
Methods
The proliferation of HeLa cells was detected by MTT. Six samples were extracted for RNA-seq transcriptome analysis. Quantitative real-time PCR (qRT-PCR) and western blotting were employed for the validation of interested target.
Results
enJSRV-Env transfection inhibited the proliferation ability of Hela cells and 170 differentially expressed genes (DEGs) were obtained by RNA-seq analysis. Among these, 5 DEGs (BHLHE41, CCN1, DLX2, DUSP6 and SH2D5) were validated by qRT-PCR, which closely related with proliferation. Western blotting analysis showed that the expression of DUSP6 and p-ERK1/2 was decreased, which suggested that ERK1/2 signaling pathway may be involved in enJSRV-Env transfection.
Conclusions
enJSRV-Env transfection inhibited the proliferation of Hela cells, probably via DUSP6 and ERK1/2 signaling pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
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