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Research article
Shaoming Ye
College of Forestry, Guangxi University
Corresponding Author
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Background: The mixing of Eucalyptus with N2-fixing trees is a frequently successful and sustainable cropping practice. In this study, we evaluated nitrogen transfer and conducted a proteomic analysis on seedlings of Eucalyptus and a N2-fixing tree, Dalbergia (D.) odorifera, from intercropping and monocropping systems, to elucidate the physiological effecting and the molecular mechanisms on N transfer of Eucalyptus mixed with D. odorifera.
Results: We demonstrated the following: (1) Nitrogen transfer occurred from D. odorifera to E. urophylla × E. grandis by 14.6 %. Interspecific facilitation of N uptake and root growth in E. urophylla × E. grandis was increased in the intercropping system, but the root growth and N absorption of D. odorifera were inhibited. (2) Among differentially expressed proteins was greater than 1.5 times, E. urophylla × E. grandis were found to be up-regulated several proteins for the nitrogen assimilation and enhancing the nitrogen competition, such as the proteins related to tricarboxylic acid/organic transformation, nitrogen metabolism and nitrogen assimilation; it also up-regulated the expression of stress resistant protein for its adaptability. However, the assimilation and metabolism of nitrogen was promoted in D. odorifera through the up-regulation of amino acid metabolism related proteins, and increased the key enzyme abundance of glycolysis pathway in D. odorifera. (3) Importantly, E. urophylla × E. grandis was the beneficiary in the process of N transfer, there were more different proteins involved in the synthesis pathway than that of the metabolic pathway, but there were more functional proteins involved in metabolic degradation in D. odorifera. Additionaly, the two groups of nitrogen compound transporter were found in E. urophylla × E. grandis, i.e. the molecular mechanism of the N transfer from D. odorifera to E. urophylla × E. grandis was explained by proteomics.
Conclusions: Our study suggests that N transfer occurred from D. odorifera to E. urophylla × E. grandis and it was affected by the variations in the differentially expressed protein. We anticipate the result can be verified in field experiments for the sustainable development of Eucalyptus plantations.
Keywords
Eucalyptus urophylla × E. grandis, Dalbergia odorifera, Rhizosphere interaction, Nitrogen uptake, Nitrogen transfer, Differentially expressed proteins
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This is a list of supplementary files associated with this preprint. Click to download.
- Schedule1.xlsx
Table S1 Differential expressed proteins ( > 1.5 times) summary in E. urophylla × E. grandis roots (p < 0.05) in this study.
- Schedule2.xlsx
Table S2 Differential expressed proteins ( > 1.5 times) summary in D. odorifera roots (p < 0.05) in this study.
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CURRENT STATUS: Under Review
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Received 08 Dec, 2020
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Received 02 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Reviewer #1 agreed
On 22 Nov, 2020
Reviewers invited
Invitations sent on 15 Nov, 2020
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On 08 Nov, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Shaoming Ye
College of Forestry, Guangxi University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 17 Nov, 2020
No community comments so far
Editorial decision: Major revision
On 13 Dec, 2020
Review #2 received
Received 08 Dec, 2020
Review #1 received
Received 02 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Reviewer #1 agreed
On 22 Nov, 2020
Reviewers invited
Invitations sent on 15 Nov, 2020
Submission checks complete
On 09 Nov, 2020
Editor assigned
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
First submitted
On 22 Oct, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The mixing of Eucalyptus with N2-fixing trees is a frequently successful and sustainable cropping practice. In this study, we evaluated nitrogen transfer and conducted a proteomic analysis on seedlings of Eucalyptus and a N2-fixing tree, Dalbergia (D.) odorifera, from intercropping and monocropping systems, to elucidate the physiological effecting and the molecular mechanisms on N transfer of Eucalyptus mixed with D. odorifera.
Results: We demonstrated the following: (1) Nitrogen transfer occurred from D. odorifera to E. urophylla × E. grandis by 14.6 %. Interspecific facilitation of N uptake and root growth in E. urophylla × E. grandis was increased in the intercropping system, but the root growth and N absorption of D. odorifera were inhibited. (2) Among differentially expressed proteins was greater than 1.5 times, E. urophylla × E. grandis were found to be up-regulated several proteins for the nitrogen assimilation and enhancing the nitrogen competition, such as the proteins related to tricarboxylic acid/organic transformation, nitrogen metabolism and nitrogen assimilation; it also up-regulated the expression of stress resistant protein for its adaptability. However, the assimilation and metabolism of nitrogen was promoted in D. odorifera through the up-regulation of amino acid metabolism related proteins, and increased the key enzyme abundance of glycolysis pathway in D. odorifera. (3) Importantly, E. urophylla × E. grandis was the beneficiary in the process of N transfer, there were more different proteins involved in the synthesis pathway than that of the metabolic pathway, but there were more functional proteins involved in metabolic degradation in D. odorifera. Additionaly, the two groups of nitrogen compound transporter were found in E. urophylla × E. grandis, i.e. the molecular mechanism of the N transfer from D. odorifera to E. urophylla × E. grandis was explained by proteomics.
Conclusions: Our study suggests that N transfer occurred from D. odorifera to E. urophylla × E. grandis and it was affected by the variations in the differentially expressed protein. We anticipate the result can be verified in field experiments for the sustainable development of Eucalyptus plantations.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- Schedule1.xlsx
Table S1 Differential expressed proteins ( > 1.5 times) summary in E. urophylla × E. grandis roots (p < 0.05) in this study.
- Schedule2.xlsx
Table S2 Differential expressed proteins ( > 1.5 times) summary in D. odorifera roots (p < 0.05) in this study.
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