Enhanced Aluminum Tolerance in Sugarcane: Evaluation of SbMATE Overexpression and Genome-wide Identification of ALMTs in Saccharum Spp.

doi:10.21203/rs.3.rs-105437/v1

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Research article

Ana Paula Ribeiro, Felipe Vinecky, KAROLINE ESTEFANI DUARTE, Thaís Ribeiro Santiago, Rafael Augusto das Chagas Noqueli Casari, Aline Forgatti Hell, Bárbara Andrade Dias Brito da Cunha, Polyana Kelly Martins, Danilo da Cruz Centeno, Geraldo Magela de Almeida Cançado, Jurandir Vieira Magalhães, Adilson Kenji Kobayashi, Wagner Rodrigo De Souza, Hugo Bruno Correa Molinari

Ana Paula Ribeiro

Embrapa Agroenergy: Embrapa Agroenergia

Felipe Vinecky

Embrapa Agroenergy: Embrapa Agroenergia

KAROLINE ESTEFANI DUARTE

Federal University of the ABC: Universidade Federal do ABC

Thaís Ribeiro Santiago

University of Brasilia: Universidade de Brasilia

Rafael Augusto das Chagas Noqueli Casari

Embrapa Agroenergy: Embrapa Agroenergia

Aline Forgatti Hell

Federal University of the ABC: Universidade Federal do ABC

Bárbara Andrade Dias Brito da Cunha

Embrapa Agroenergia

Polyana Kelly Martins

Embrapa Agroenergy: Embrapa Agroenergia

Danilo da Cruz Centeno

Federal University of the ABC: Universidade Federal do ABC

Geraldo Magela de Almeida Cançado

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Jurandir Vieira Magalhães

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Adilson Kenji Kobayashi

Embrapa Agroenergy: Embrapa Agroenergia

Wagner Rodrigo De Souza

Federal University of the ABC: Universidade Federal do ABC

Hugo Bruno Correa Molinari

Genetics and Biotechnology Laboratory, Embrapa Agroenergy, Brasilia, DF, Brazil

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9951-8229

DOI:

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Background

A major limiting factor for plant growth is the aluminum (Al) toxicity in acidic soils, especially in tropical regions. The exclusion of Al from the root apex through root exudation of organic acids such as malate and citrate are both the most ubiquitous tolerance mechanisms in the plant kingdom. Two families of anion channels that confer Al tolerance are well described in the literature, ALMT and MATE family.

Results

In this study, sugarcane plants constitutively overexpressing the Sorghum bicolor MATE gene (SbMATE) showed improved tolerance to Al when compared to non-transgenic (NT) plants, characterized by sustained root growth and exclusion of aluminum from the root apex based on the result obtained with hematoxylin staining. In addition, genome-wide analysis of the recently released sugarcane genome identified 11 ALMT genes and molecular studies showed potential new targets for aluminum tolerance.

Conclusions

Our results indicate that the transgenic plants overexpressing the Sorghum bicolor MATE has an improved tolerance to Al. The expression profile of ALMT genes revels potential candidate genes to be used has an alternative for agricultural expansion in Brazil and other areas with aluminum toxicity in poor and acid soils.

Keywords

aluminum, sugarcane, MATE, ALMT, abiotic stress, hydroponic system

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CURRENT STATUS: Published

View the published article at BMC Plant Biology.

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Posted 17 Nov, 2020

No community comments so far
Editorial decision: Major revision
On 27 Dec, 2020
Review #2 received
Received 20 Dec, 2020
Review #1 received
Received 05 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Editor assigned
On 09 Nov, 2020
Reviewers invited
Invitations sent on 09 Nov, 2020
Submission checks complete
On 09 Nov, 2020
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On 08 Nov, 2020
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On 23 Oct, 2020

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Subject Areas

Plant Physiology and Morphology

Plant Molecular Biology and Genetics

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See the published version of this preprint at BMC Plant Biology.

This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.

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Research article

Ana Paula Ribeiro, Felipe Vinecky, KAROLINE ESTEFANI DUARTE, Thaís Ribeiro Santiago, Rafael Augusto das Chagas Noqueli Casari, Aline Forgatti Hell, Bárbara Andrade Dias Brito da Cunha, Polyana Kelly Martins, Danilo da Cruz Centeno, Geraldo Magela de Almeida Cançado, Jurandir Vieira Magalhães, Adilson Kenji Kobayashi, Wagner Rodrigo De Souza, Hugo Bruno Correa Molinari

Ana Paula Ribeiro

Embrapa Agroenergy: Embrapa Agroenergia

Felipe Vinecky

Embrapa Agroenergy: Embrapa Agroenergia

KAROLINE ESTEFANI DUARTE

Federal University of the ABC: Universidade Federal do ABC

Thaís Ribeiro Santiago

University of Brasilia: Universidade de Brasilia

Rafael Augusto das Chagas Noqueli Casari

Embrapa Agroenergy: Embrapa Agroenergia

Aline Forgatti Hell

Federal University of the ABC: Universidade Federal do ABC

Bárbara Andrade Dias Brito da Cunha

Embrapa Agroenergia

Polyana Kelly Martins

Embrapa Agroenergy: Embrapa Agroenergia

Danilo da Cruz Centeno

Federal University of the ABC: Universidade Federal do ABC

Geraldo Magela de Almeida Cançado

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Jurandir Vieira Magalhães

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Adilson Kenji Kobayashi

Embrapa Agroenergy: Embrapa Agroenergia

Wagner Rodrigo De Souza

Federal University of the ABC: Universidade Federal do ABC

Hugo Bruno Correa Molinari

Genetics and Biotechnology Laboratory, Embrapa Agroenergy, Brasilia, DF, Brazil

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9951-8229

Badges

Prescreen

Peer Review Timeline

CURRENT STATUS: Published

View the published article at BMC Plant Biology.

Version 1

Posted 17 Nov, 2020

No community comments so far
Editorial decision: Major revision
On 27 Dec, 2020
Review #2 received
Received 20 Dec, 2020
Review #1 received
Received 05 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Editor assigned
On 09 Nov, 2020
Reviewers invited
Invitations sent on 09 Nov, 2020
Submission checks complete
On 09 Nov, 2020
Editor invited
On 08 Nov, 2020
First submitted
On 23 Oct, 2020

MetricsComments: 0PDF Downloads: ...HTML Views: ...

Subject Areas

Plant Physiology and Morphology

Plant Molecular Biology and Genetics

DOI:

10.21203/rs.3.rs-105437/v1

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY 4.0 License. Read Full License

View the published article at BMC Plant Biology.

Background

Results

Conclusions

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

This is a list of supplementary files associated with this preprint. Click to download.

See the published version of this preprint at BMC Plant Biology.

Learn more about In Review

A new preprint design is coming!

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Research article

Ana Paula Ribeiro

Embrapa Agroenergy: Embrapa Agroenergia

Felipe Vinecky

Embrapa Agroenergy: Embrapa Agroenergia

KAROLINE ESTEFANI DUARTE

Federal University of the ABC: Universidade Federal do ABC

Thaís Ribeiro Santiago

University of Brasilia: Universidade de Brasilia

Rafael Augusto das Chagas Noqueli Casari

Embrapa Agroenergy: Embrapa Agroenergia

Aline Forgatti Hell

Federal University of the ABC: Universidade Federal do ABC

Bárbara Andrade Dias Brito da Cunha

Embrapa Agroenergia

Polyana Kelly Martins

Embrapa Agroenergy: Embrapa Agroenergia

Danilo da Cruz Centeno

Federal University of the ABC: Universidade Federal do ABC

Geraldo Magela de Almeida Cançado

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Jurandir Vieira Magalhães

Embrapa: Empresa Brasileira de Pesquisa Agropecuaria

Adilson Kenji Kobayashi

Embrapa Agroenergy: Embrapa Agroenergia

Wagner Rodrigo De Souza

Federal University of the ABC: Universidade Federal do ABC

Hugo Bruno Correa Molinari

Genetics and Biotechnology Laboratory, Embrapa Agroenergy, Brasilia, DF, Brazil

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9951-8229

DOI:

10.21203/rs.3.rs-105437/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

aluminum, sugarcane, MATE, ALMT, abiotic stress, hydroponic system

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Peer Review Timeline

CURRENT STATUS: Published

View the published article at BMC Plant Biology.

Version 1

No community comments so far

Editorial decision: Major revision

Review #2 received

Review #1 received

Reviewer #2 agreed

Reviewer #1 agreed

Editor assigned

Reviewers invited

Submission checks complete

Editor invited

First submitted

Metrics

Comments: 0

PDF Downloads: ...

HTML Views: ...