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Research article
Margarete Baier
Freie Universität Berlin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1687-8857
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background The majority of stress-sensitive genes responds to cold and highlight in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4 °C cold stimulus modifies cold regulation of gene expression for up to a week at 20 °C, although the primary cold effects are reverted within the first 24 h. Such memory based regulation is called priming. Here, we show the effect of 24 h cold priming on cold regulation of gene expression on a transcriptome-wide scale and test if and how cold priming effects light regulation of gene expression.
Results 304 genes were differently regulated between cold-primed and non-primed plants after a second 24 h long 4 °C cold treatment. After triggering the plants with a heat-filtered highlight stimulus (800 µmol quanta m -2 s -1 ), 1011 genes showed priming dependent regulation. Only 32 of the priming-sensitive genes responded similarly to cold and light triggering. The majority of the priming-sensitive genes were regulated in a stressor-specific manner. 29 genes were even inversely regulated by the two triggering stimuli. Cold priming preferentially supported expression of genes involved in the defense against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defense genes in response to highlight triggering. qPCR in several independently cultivated and treated samples confirmed the trends observed by RNA-Sequencing.
Conclusion The 24 h long priming cold stimulus activates a several day lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defense regulation in a stressor-specific manner.
Keywords
cold, light, priming, triggering, memory, Arabidopsis thaliana, growth, defense, gene expression
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editorial decision: Accept
On 09 Jun, 2020
Editor assigned
On 04 Jun, 2020
Submission checks complete
On 03 Jun, 2020
Editor invited
On 03 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 02 Jun, 2020
Editor assigned
On 24 May, 2020
Submission checks complete
On 23 May, 2020
Editor invited
On 23 May, 2020
No comments provided
Editorial decision: Minor revision
On 20 May, 2020
Review #1 received
Received 14 May, 2020
Reviewers invited
Invitations sent on 08 May, 2020
Reviewer #1 agreed
On 08 May, 2020
Editor assigned
On 07 May, 2020
Editor invited
On 06 May, 2020
Submission checks complete
On 30 Dec, 2019
No comments provided
Review #2 received
Received 04 May, 2020
Editorial decision: Minor revision
On 04 May, 2020
Reviewer #2 agreed
On 06 Apr, 2020
Reviewer #1 agreed
On 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
Reviewers invited
Invitations sent on 27 Mar, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
Version 1
Posted 06 Jan, 2020
No comments provided
Editorial decision: Major revision
On 12 Mar, 2020
Review #2 received
Received 10 Mar, 2020
Reviewer #2 agreed
On 23 Feb, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #1 agreed
On 09 Jan, 2020
Reviewers invited
Invitations sent on 08 Jan, 2020
Editor invited
On 31 Dec, 2019
Editor assigned
On 11 Dec, 2019
First submitted
On 10 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Margarete Baier
Freie Universität Berlin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1687-8857
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editorial decision: Accept
On 09 Jun, 2020
Editor assigned
On 04 Jun, 2020
Submission checks complete
On 03 Jun, 2020
Editor invited
On 03 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 02 Jun, 2020
Editor assigned
On 24 May, 2020
Submission checks complete
On 23 May, 2020
Editor invited
On 23 May, 2020
No comments provided
Editorial decision: Minor revision
On 20 May, 2020
Review #1 received
Received 14 May, 2020
Reviewers invited
Invitations sent on 08 May, 2020
Reviewer #1 agreed
On 08 May, 2020
Editor assigned
On 07 May, 2020
Editor invited
On 06 May, 2020
Submission checks complete
On 30 Dec, 2019
No comments provided
Review #2 received
Received 04 May, 2020
Editorial decision: Minor revision
On 04 May, 2020
Reviewer #2 agreed
On 06 Apr, 2020
Reviewer #1 agreed
On 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
Reviewers invited
Invitations sent on 27 Mar, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
Version 1
Posted 06 Jan, 2020
No comments provided
Editorial decision: Major revision
On 12 Mar, 2020
Review #2 received
Received 10 Mar, 2020
Reviewer #2 agreed
On 23 Feb, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #1 agreed
On 09 Jan, 2020
Reviewers invited
Invitations sent on 08 Jan, 2020
Editor invited
On 31 Dec, 2019
Editor assigned
On 11 Dec, 2019
First submitted
On 10 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background The majority of stress-sensitive genes responds to cold and highlight in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4 °C cold stimulus modifies cold regulation of gene expression for up to a week at 20 °C, although the primary cold effects are reverted within the first 24 h. Such memory based regulation is called priming. Here, we show the effect of 24 h cold priming on cold regulation of gene expression on a transcriptome-wide scale and test if and how cold priming effects light regulation of gene expression.
Results 304 genes were differently regulated between cold-primed and non-primed plants after a second 24 h long 4 °C cold treatment. After triggering the plants with a heat-filtered highlight stimulus (800 µmol quanta m -2 s -1 ), 1011 genes showed priming dependent regulation. Only 32 of the priming-sensitive genes responded similarly to cold and light triggering. The majority of the priming-sensitive genes were regulated in a stressor-specific manner. 29 genes were even inversely regulated by the two triggering stimuli. Cold priming preferentially supported expression of genes involved in the defense against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defense genes in response to highlight triggering. qPCR in several independently cultivated and treated samples confirmed the trends observed by RNA-Sequencing.
Conclusion The 24 h long priming cold stimulus activates a several day lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defense regulation in a stressor-specific manner.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
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