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Research article
Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana
Margarete Baier
Freie Universität Berlin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1687-8857
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: The majority of stress-sensitive genes responds to cold and high light in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4 °C cold stimulus modifies cold regulation of gene expression for up to a week at 20 °C, although the primary cold effects are reverted within the first 24 h. Such memory-based regulation is called priming. Here, we analyse the effect of 24 h cold priming on cold regulation of gene expression on a transcriptome-wide scale and investigate if and how cold priming affects light regulation of gene expression.
Results: Cold-priming affected cold and excess light regulation of a small subset of genes. In contrast to the strong gene co-regulation observed upon cold and light stress in not-primed plants, most priming-sensitive genes were regulated in a stressor-specific manner in cold-primed plant. Furthermore, almost as much genes were inversely regulated as co-regulated by a 24 h long 4 °C cold treatment and exposure to heat-filtered high light (800 µmol quanta m-2 s-1). Gene ontology enrichment analysis revealed that cold priming preferentially supports expression of genes involved in the defence against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defence genes in response to high light triggering. qPCR with independently cultivated and treated replicates confirmed the trends observed in the RNASeq guide experiment.
Conclusion: A 24 h long priming cold stimulus activates a several days lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defence regulation in a stressor-specific manner.
Keywords
cold, light, priming, triggering, memory, Arabidopsis thaliana, growth, defence, gene expression
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Editor invited
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Editorial decision: Minor revision
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Reviewers invited
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On 08 May, 2020
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On 07 May, 2020
Editor invited
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Review #2 received
Received 04 May, 2020
Editorial decision: Minor revision
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On 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
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On 24 Mar, 2020
Editor invited
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Editorial decision: Major revision
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Review #2 received
Received 10 Mar, 2020
Reviewer #2 agreed
On 23 Feb, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #1 agreed
On 09 Jan, 2020
Reviewers invited
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Editor invited
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Editor assigned
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana
Margarete Baier
Freie Universität Berlin
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1687-8857
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 5
Posted 10 Jun, 2020
Published
On 18 Jun, 2020
No community comments so far
Editorial decision: Accept
On 09 Jun, 2020
Editor assigned
On 04 Jun, 2020
Submission checks complete
On 03 Jun, 2020
Editor invited
On 03 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 02 Jun, 2020
Editor assigned
On 24 May, 2020
Submission checks complete
On 23 May, 2020
Editor invited
On 23 May, 2020
No comments provided
Editorial decision: Minor revision
On 20 May, 2020
Review #1 received
Received 14 May, 2020
Reviewers invited
Invitations sent on 08 May, 2020
Reviewer #1 agreed
On 08 May, 2020
Editor assigned
On 07 May, 2020
Editor invited
On 06 May, 2020
Submission checks complete
On 30 Dec, 2019
No comments provided
Review #2 received
Received 04 May, 2020
Editorial decision: Minor revision
On 04 May, 2020
Reviewer #2 agreed
On 06 Apr, 2020
Reviewer #1 agreed
On 30 Mar, 2020
Review #1 received
Received 30 Mar, 2020
Reviewers invited
Invitations sent on 27 Mar, 2020
Editor assigned
On 25 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
No comments provided
Editorial decision: Major revision
On 12 Mar, 2020
Review #2 received
Received 10 Mar, 2020
Reviewer #2 agreed
On 23 Feb, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #1 agreed
On 09 Jan, 2020
Reviewers invited
Invitations sent on 08 Jan, 2020
Editor invited
On 31 Dec, 2019
Editor assigned
On 11 Dec, 2019
First submitted
On 10 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: The majority of stress-sensitive genes responds to cold and high light in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4 °C cold stimulus modifies cold regulation of gene expression for up to a week at 20 °C, although the primary cold effects are reverted within the first 24 h. Such memory-based regulation is called priming. Here, we analyse the effect of 24 h cold priming on cold regulation of gene expression on a transcriptome-wide scale and investigate if and how cold priming affects light regulation of gene expression.
Results: Cold-priming affected cold and excess light regulation of a small subset of genes. In contrast to the strong gene co-regulation observed upon cold and light stress in not-primed plants, most priming-sensitive genes were regulated in a stressor-specific manner in cold-primed plant. Furthermore, almost as much genes were inversely regulated as co-regulated by a 24 h long 4 °C cold treatment and exposure to heat-filtered high light (800 µmol quanta m-2 s-1). Gene ontology enrichment analysis revealed that cold priming preferentially supports expression of genes involved in the defence against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defence genes in response to high light triggering. qPCR with independently cultivated and treated replicates confirmed the trends observed in the RNASeq guide experiment.
Conclusion: A 24 h long priming cold stimulus activates a several days lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defence regulation in a stressor-specific manner.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10