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Research article
Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer
Hongjie Li
jilinnongyedaxue
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3537-673X
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Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng.
Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied.
Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.
Keywords
Panax ginseng, bZIP transcription factor, Phylogenetic analysis, Functional differentiation, Drought stress
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CURRENT STATUS: Under Review
Version 1
Posted 03 Jan, 2020
No community comments so far
Editorial decision: Major revision
On 07 Feb, 2021
Review #3 received
Received 16 Dec, 2020
Review #2 received
Received 15 Dec, 2020
Reviewer #3 agreed
On 07 Dec, 2020
Review #1 received
Received 23 Jan, 2020
Reviewers invited
Invitations sent on 13 Jan, 2020
Reviewer #1 agreed
On 13 Jan, 2020
Reviewer #2 agreed
On 13 Jan, 2020
Editor assigned
On 10 Jan, 2020
Submission checks complete
On 31 Dec, 2019
Editor invited
On 30 Dec, 2019
First submitted
On 21 Dec, 2019
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer
Hongjie Li
jilinnongyedaxue
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3537-673X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 03 Jan, 2020
No community comments so far
Editorial decision: Major revision
On 07 Feb, 2021
Review #3 received
Received 16 Dec, 2020
Review #2 received
Received 15 Dec, 2020
Reviewer #3 agreed
On 07 Dec, 2020
Review #1 received
Received 23 Jan, 2020
Reviewers invited
Invitations sent on 13 Jan, 2020
Reviewer #1 agreed
On 13 Jan, 2020
Reviewer #2 agreed
On 13 Jan, 2020
Editor assigned
On 10 Jan, 2020
Submission checks complete
On 31 Dec, 2019
Editor invited
On 30 Dec, 2019
First submitted
On 21 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng.
Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied.
Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.