See the published version of this preprint at Plant Methods.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Methodology
Luis Rafael Herrera-Estrella
Cinvestav
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7936-3856
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Utricularia genus belongs to Lentibulariaceae family and has the highest number of species including terrestrials, epiphytes and aquatics plants. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologist since the early studies of Darwin. Utricularia gibba is an aquatic carnivorous plant with sophisticated bladders traps that have one of the most complex suction mechanisms for trapping a prey. However, the molecular characterization of trap developmental and the biophysical processes involved in prey trapping are still largely unknown due to the lack of simple and reproducible gene transfer system for carnivorous plants.
Results Here, we report the establishment of a simple, fast and effective protocol for the genetic transformation of U. gibba based on the T-DNA of Agrobacterium tumefaciens . An in vitro selection system using Phosphinotricin as selective agent was established for U. gibba. We report the tissue specific expression of the 35S promoter and the promoter of a trap specific ribonuclease gene. Plant transformation was confirmed by PCR and Real Time PCR in U. gibba plants.
Conclusions We conclude that the genetic transformation protocol we developed is an effective method to study developmental biology and functional genomics of carnivory and propose U. gibba as model to study the developmental processes involved in trap formation.
Keywords
Agrobacterium tumefaciens, genetic transformation, carnivorous plant
The full text of this article is available to read as a PDF.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Plant Methods.
No community comments so far
Editorial decision: Accept
On 29 Mar, 2020
Editor assigned
On 28 Mar, 2020
Submission checks complete
On 27 Mar, 2020
Editor invited
On 27 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 24 Mar, 2020
Review #1 received
Received 23 Mar, 2020
Reviewer #2 agreed
On 11 Mar, 2020
Review #2 received
Received 11 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
Version 1
Posted 07 Jan, 2020
No comments provided
Review #2 received
Received 12 Feb, 2020
Editorial decision: Major revision
On 12 Feb, 2020
Reviewer #2 agreed
On 23 Jan, 2020
Reviewer #1 agreed
On 20 Jan, 2020
Review #1 received
Received 20 Jan, 2020
Reviewers invited
Invitations sent on 12 Jan, 2020
Editor assigned
On 04 Jan, 2020
Submission checks complete
On 03 Jan, 2020
Editor invited
On 03 Jan, 2020
First submitted
On 02 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Plant Methods.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Methodology
Luis Rafael Herrera-Estrella
Cinvestav
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7936-3856
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Plant Methods.
No community comments so far
Editorial decision: Accept
On 29 Mar, 2020
Editor assigned
On 28 Mar, 2020
Submission checks complete
On 27 Mar, 2020
Editor invited
On 27 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 24 Mar, 2020
Review #1 received
Received 23 Mar, 2020
Reviewer #2 agreed
On 11 Mar, 2020
Review #2 received
Received 11 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
Version 1
Posted 07 Jan, 2020
No comments provided
Review #2 received
Received 12 Feb, 2020
Editorial decision: Major revision
On 12 Feb, 2020
Reviewer #2 agreed
On 23 Jan, 2020
Reviewer #1 agreed
On 20 Jan, 2020
Review #1 received
Received 20 Jan, 2020
Reviewers invited
Invitations sent on 12 Jan, 2020
Editor assigned
On 04 Jan, 2020
Submission checks complete
On 03 Jan, 2020
Editor invited
On 03 Jan, 2020
First submitted
On 02 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Plant Methods.
Background Utricularia genus belongs to Lentibulariaceae family and has the highest number of species including terrestrials, epiphytes and aquatics plants. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologist since the early studies of Darwin. Utricularia gibba is an aquatic carnivorous plant with sophisticated bladders traps that have one of the most complex suction mechanisms for trapping a prey. However, the molecular characterization of trap developmental and the biophysical processes involved in prey trapping are still largely unknown due to the lack of simple and reproducible gene transfer system for carnivorous plants.
Results Here, we report the establishment of a simple, fast and effective protocol for the genetic transformation of U. gibba based on the T-DNA of Agrobacterium tumefaciens . An in vitro selection system using Phosphinotricin as selective agent was established for U. gibba. We report the tissue specific expression of the 35S promoter and the promoter of a trap specific ribonuclease gene. Plant transformation was confirmed by PCR and Real Time PCR in U. gibba plants.
Conclusions We conclude that the genetic transformation protocol we developed is an effective method to study developmental biology and functional genomics of carnivory and propose U. gibba as model to study the developmental processes involved in trap formation.
The full text of this article is available to read as a PDF.
Loading...