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Research article
Zhiying Zhang
Northwest Agriculture and Forestry University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The baker’s yeast, saccharomyces cerevisiae, has been widely used throughout our daily life in diverse aspects for thousands of years. The saccharomyces cerevisiae was found to specifically target the dendritic cells (DCs) in mammalian with a manner of antigen-receptor interaction as described previously. It is necessary to investigate the effect of the baker’s yeasts on global gene expression dynamics of intestinal DCs and explore the possibilities of using baker’s yeast as gene delivery vehicle to modulate animal’s immune functions
Results
with a murine oral delivery model in vivo, we confirmed the feasibility of using budding yeast as gene delivery vehicle to the intestinal DCs using the Western blots. We then examined the transcriptome profile of the mouse intestinal DCs upon yeast stimulus. The enrichment analysis of unique transcripts indicated the beneficial role of yeast in modulating the DC-mediated adaptive immunity. Compared with previous study, we also found that a large fraction of the regulated genes is coincident with the response induced by other fungus, suggesting that the budding yeast induces a similar tailored unique genetic re-programming of DCs. Another analysis of transcriptome profile indicated that expression of β-catenin gene significantly changes DCs gene expression related to inflammatory response and cell adhesion.
Conclusions
Here, we defined the role of budding yeast on global gene expression of intestinal DCs, and confirmed the important role of β-catenin gene on the DCs-related inflammatory response, which provides a framework for the development of mucosa yeast-based DNA vaccine.
Keywords
intestinal DCs, yeast, β-catenin, adaptive immunity, RNA-seq
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CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Review #1 received
Received 14 Sep, 2019
Reviewer #1 agreed
On 29 Aug, 2019
Reviewers invited
Invitations sent on 22 Aug, 2019
Editor assigned
On 10 Jul, 2019
Submission checks complete
On 09 Jul, 2019
Editor invited
On 09 Jul, 2019
No comments provided
Editorial decision: Minor revision
On 08 Jul, 2019
Submission checks complete
On 19 Jun, 2019
Editor invited
On 19 Jun, 2019
Editor assigned
On 19 Jun, 2019
Version 1
Posted 26 May, 2019
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 17 Jun, 2019
Submission checks complete
On 14 May, 2019
Editor invited
On 14 May, 2019
Editor assigned
On 14 May, 2019
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Zhiying Zhang
Northwest Agriculture and Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Review #1 received
Received 14 Sep, 2019
Reviewer #1 agreed
On 29 Aug, 2019
Reviewers invited
Invitations sent on 22 Aug, 2019
Editor assigned
On 10 Jul, 2019
Submission checks complete
On 09 Jul, 2019
Editor invited
On 09 Jul, 2019
No comments provided
Editorial decision: Minor revision
On 08 Jul, 2019
Submission checks complete
On 19 Jun, 2019
Editor invited
On 19 Jun, 2019
Editor assigned
On 19 Jun, 2019
Version 1
Posted 26 May, 2019
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 17 Jun, 2019
Submission checks complete
On 14 May, 2019
Editor invited
On 14 May, 2019
Editor assigned
On 14 May, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background
The baker’s yeast, saccharomyces cerevisiae, has been widely used throughout our daily life in diverse aspects for thousands of years. The saccharomyces cerevisiae was found to specifically target the dendritic cells (DCs) in mammalian with a manner of antigen-receptor interaction as described previously. It is necessary to investigate the effect of the baker’s yeasts on global gene expression dynamics of intestinal DCs and explore the possibilities of using baker’s yeast as gene delivery vehicle to modulate animal’s immune functions
Results
with a murine oral delivery model in vivo, we confirmed the feasibility of using budding yeast as gene delivery vehicle to the intestinal DCs using the Western blots. We then examined the transcriptome profile of the mouse intestinal DCs upon yeast stimulus. The enrichment analysis of unique transcripts indicated the beneficial role of yeast in modulating the DC-mediated adaptive immunity. Compared with previous study, we also found that a large fraction of the regulated genes is coincident with the response induced by other fungus, suggesting that the budding yeast induces a similar tailored unique genetic re-programming of DCs. Another analysis of transcriptome profile indicated that expression of β-catenin gene significantly changes DCs gene expression related to inflammatory response and cell adhesion.
Conclusions
Here, we defined the role of budding yeast on global gene expression of intestinal DCs, and confirmed the important role of β-catenin gene on the DCs-related inflammatory response, which provides a framework for the development of mucosa yeast-based DNA vaccine.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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