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Research Article
Aleksandr Ilinov
Niigata University
Corresponding Author
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DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles on the outside as an extracellular DNA (eDNA), an essential component for the formation of antibiotic tolerance bacteria in biofilm. Mycobacteria include life-threating human pathogens, most of which are slow growers. However, little is known about the nature of pathogenic mycobacteria’s eDNA. Here we found that eDNA is present in slow growers of mycobacterial pathogens, such as Mycobacterium tuberculosis, M. intracellulare, and M. avium at exponential growth phase. In contrast, eDNA is little in all tested rapid growers of mycobacteria. The physiological impact of disrupted eDNA on slow growers of mycobacteria include reduced formation of pellicle, a floating biofilm, and enhanced susceptibility to isoniazid and amikacin. Isolation and sequencing of eDNA revealed that it is identical to the genomic DNA in M. tuberculosis and M. intracellulare. In contrast, accumulation of phage DNA in eDNA of M. avium, suggests that the DNA released differs among mycobacterial species. Our data show important functions of eDNA for biofilm formation and drug tolerance in slow growers of mycobacteria.
Keywords
DNA, eDNA, cellular organisms, Mycobacteria
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CURRENT STATUS: Under Review
Version 1
Posted 02 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 10 Dec, 2020
Reviews received
Received 08 Dec, 2020
Reviewers agreed
On 27 Nov, 2020
Reviewers invited
Invitations sent on 27 Nov, 2020
Editor assigned
On 27 Nov, 2020
Editor invited
On 27 Nov, 2020
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On 27 Nov, 2020
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Aleksandr Ilinov
Niigata University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 02 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 10 Dec, 2020
Reviews received
Received 08 Dec, 2020
Reviewers agreed
On 27 Nov, 2020
Reviewers invited
Invitations sent on 27 Nov, 2020
Editor assigned
On 27 Nov, 2020
Editor invited
On 27 Nov, 2020
Submission checks complete
On 27 Nov, 2020
First submitted
On 16 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles on the outside as an extracellular DNA (eDNA), an essential component for the formation of antibiotic tolerance bacteria in biofilm. Mycobacteria include life-threating human pathogens, most of which are slow growers. However, little is known about the nature of pathogenic mycobacteria’s eDNA. Here we found that eDNA is present in slow growers of mycobacterial pathogens, such as Mycobacterium tuberculosis, M. intracellulare, and M. avium at exponential growth phase. In contrast, eDNA is little in all tested rapid growers of mycobacteria. The physiological impact of disrupted eDNA on slow growers of mycobacteria include reduced formation of pellicle, a floating biofilm, and enhanced susceptibility to isoniazid and amikacin. Isolation and sequencing of eDNA revealed that it is identical to the genomic DNA in M. tuberculosis and M. intracellulare. In contrast, accumulation of phage DNA in eDNA of M. avium, suggests that the DNA released differs among mycobacterial species. Our data show important functions of eDNA for biofilm formation and drug tolerance in slow growers of mycobacteria.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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