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Research Article
Jinlin Guo
Chengdu University of Traditional Chinese Medicine
Corresponding Author
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Ophiocordyceps sinensis has been a source of valuable materials in traditional Asian medicine for over two thousand years. With recent global warming and overharvest, however, the availability of these wild fungi has decreased dramatically. While fruiting bodies of O. sinensis have been artificially cultivated, the molecular mechanisms that govern the induction of fruiting bodies at the transcriptional and post-transcriptional levels are unclear. In this study, we carried out both mRNA and small RNA sequencing to identify crucial genes and miRNA-like RNAs (milRNAs) involved in the development of fruiting bodies. A total of 2875 differentially expressed genes (DEGs) and 71 differentially expressed milRNAs (DEMs) were identified among the mycoparasite complex (MC), the sclerotium (ST) and the fruiting body (FB) stage. Functional enrichment and Gene Set Enrichment Analysis, analyses indicated that the ST had increased oxidative stress and energy metabolism, and that mitogen-activated protein kinase signaling might induce the formation of fruiting bodies. Integrated analysis of DEGs and DEMs revealed that n_os_milR16, n_os_milR21, n_os_milR34, and n_os_milR90 could be candidate milRNAs that regulate the induction of fruiting bodies. This study provides transcriptome-wide insight into the molecular basis of fruiting body formation in O. Sinensis, and identifies potential candidate genes for improving induction rate.
Keywords
Ophiocordyceps sinensis, miRNA, transcriptome-wide, sinensis
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CURRENT STATUS: Under Review
Version 1
Posted 03 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 23 Feb, 2021
Reviews received
Received 30 Jan, 2021
Reviewers agreed
On 21 Jan, 2021
Reviewers agreed
On 11 Jan, 2021
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor assigned
On 02 Dec, 2020
Editor invited
On 02 Dec, 2020
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On 01 Dec, 2020
First submitted
On 17 Nov, 2020
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A new preprint design is coming!
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Jinlin Guo
Chengdu University of Traditional Chinese Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 03 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 23 Feb, 2021
Reviews received
Received 30 Jan, 2021
Reviewers agreed
On 21 Jan, 2021
Reviewers agreed
On 11 Jan, 2021
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor assigned
On 02 Dec, 2020
Editor invited
On 02 Dec, 2020
Submission checks complete
On 01 Dec, 2020
First submitted
On 17 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Ophiocordyceps sinensis has been a source of valuable materials in traditional Asian medicine for over two thousand years. With recent global warming and overharvest, however, the availability of these wild fungi has decreased dramatically. While fruiting bodies of O. sinensis have been artificially cultivated, the molecular mechanisms that govern the induction of fruiting bodies at the transcriptional and post-transcriptional levels are unclear. In this study, we carried out both mRNA and small RNA sequencing to identify crucial genes and miRNA-like RNAs (milRNAs) involved in the development of fruiting bodies. A total of 2875 differentially expressed genes (DEGs) and 71 differentially expressed milRNAs (DEMs) were identified among the mycoparasite complex (MC), the sclerotium (ST) and the fruiting body (FB) stage. Functional enrichment and Gene Set Enrichment Analysis, analyses indicated that the ST had increased oxidative stress and energy metabolism, and that mitogen-activated protein kinase signaling might induce the formation of fruiting bodies. Integrated analysis of DEGs and DEMs revealed that n_os_milR16, n_os_milR21, n_os_milR34, and n_os_milR90 could be candidate milRNAs that regulate the induction of fruiting bodies. This study provides transcriptome-wide insight into the molecular basis of fruiting body formation in O. Sinensis, and identifies potential candidate genes for improving induction rate.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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