3.1 Homeopathic potency measurement
To probe the electromagnetic signature generated from the homeopathic medicine samples prepared of FM-1X to FM-6X potencies. Initially at the low excitation frequency was used to trigger the homeopathic medicine samples. Figure 3a shows the variation of the output voltage detected from the test samples at 300 Hz excitation frequency. It is clear from Figure 3a, that the output voltage of about 65.62 µV was produced from the FM-1X homeopathy under a frequency of 300 Hz. It was observed that the increase in the potency of FM homeopathic medicine from 1X to 6X, the output response voltage under magnetic field gets reduced. Figure 3 also shows the output voltage generated from the homeopathic medicine samples of FM-1X, FM-2X, FM-3X, FM-4X, FM-5X, and FM-6X potencies. The output voltage signals obtained were of the order of 65.62, 64.24, 63.65, 63.46, 63.16, 63.13 µV, respectively. This is the first experimental investigation of the homeopathic medicine samples under a low-frequency excitation (300 Hz) electromagnetic field. The decrease in the output voltage at higher potencies of the homeopathic medicine is related to the concentration and morphology of the FM homeopathic medicine. The low output voltage at high potencies may be attributed to the low concentration of FM in the 6X potencies. It is worth pointing out that the base α‑lactose monohydrate used in the preparation of homeopathic medicine does not exhibit any magnetic property. Despite the absence of magnetic property of homeopathic medicine, the generation of the output voltage at a certain resonance frequency shows that homeopathic medicine has a healing mechanism associated with it. Moreover, the clear detection of output voltage from various potencies FM-1X to FM-6X proves the importance of electromagnetic resonance-based detection of homeopathic medicines with different potencies. However, the output voltage signal received from the low potencies of FM-5X and FM-6X almost coincided at excitation frequency (Fig. 3b). It was also reported that excitation frequency (resonance) strongly influenced the output voltage or electromagnetic response from the homeopathic potencies. It can be clearly observed that the homeopathic potencies have a larger difference in the successive homeopathic potency peak voltages. The largest difference in the peak voltage was observed in the case of the 1X and 2X potencies. This difference reduces as the potency level increased from 1X to 6X. The difference became almost insignificant in 5X and 6X potencies as seen in Figure 3b. Thus, it may be pointed here that as the homeopathic potency increases they may be required to get excited at higher resonating frequencies. Therefore, to better resolve the higher potency, the investigation was conducted for the identification of an appropriate upper range of lower excitation frequency.
Figure 3. (a) Electromagnetic signals/output voltage from various Ferrum Metallicum samples under excitation frequency of 300 Hz and (b) an enlarged view of the Electromagnetic signals/output voltage displaying a small noticeable difference between 5X and 6X potencies. The output voltage from the FM-1X, FM-4X, and FM-6X are measured under various frequencies ranging from 300 Hz to 60 kHz. Figure 4 shows the variation of the output voltage under different excitation frequencies. The output curve indicates that the output voltage generated from each FM sample varies with the applied frequency. However, the output voltage increases with the frequency and the response curve almost flattens from 3 kHz to 8 kHz. The response curve of the potencies maintained constant differences in this frequency range. However, the difference starts to fluctuate as the frequency is increased beyond 4.8 kHz. Thus, the excitation frequency was selected for further investigation to resolve the higher potencies.
Figure 4. Electromagnetic signals/output voltage from Ferrum Metallicum sample of different potencies at various frequency ranging from 300 Hz to 60000 Hz.
Figure 5. (a) Electromagnetic signals/output voltage from Ferrum Metallicum sample under excitation frequency of 4.8 kHz and (b) an enlarged view of the Electromagnetic signals/output voltage displaying a larger noticeable difference between 5X and 6X potencies.
To further investigate the electromagnetic signature in different homeopathic medicines at 4.8KHz excitation frequency, a set of 3X potencies medicine samples such as Zincum Metallicum (ZM), Argentum Metallicum (AM), Alumina (AL), Antim-Tart (AT), Ferrum Metallicum (FM) and Plumbum Metallicum (PM) are prepared. Figure 6 shows the generated electromagnetic signal from the medicine sample ZM-3X, AM-3X, AL-3X, AT-3X, FM-3X and PM-3X, respectively. The obtained voltage signals were of the order of 148.40, 148.44, 148.87, 149.00, 149.80, 150.65 µV, respectively. The result clearly suggests that each homeopathic medicine has distinct characteristic electromagnetic property and thus, generated different output voltage prepared through a similar standard protocol of homeopathic medicinal preparation.
Figure 6. Electromagnetic signals/output voltage from various homeopathy sample under excitation frequency of 4.8 kHz.
The important results obtained were that each of the homeopathic potency generates a characteristic signal in each case whether the starting materials are of a magnetic or non-magnetic nature. The theory suggested by Lenger40 that the succussion and vibrations during the preparation of the homeopathic potency lead to the generation of the electromagnetic property in the homeopathic potency. Hence, it can thus be proved that the succussion and vibrations during the preparation of the homeopathic medicines lead to the generation of a magnetic character in each of the homeopathic potency. The homeopathic medicine photons are bound by the magnetic field of the base material i.e. α‑lactose monohydrate (milk sugar). However, it is also important to discuss about the strength of the signals obtained in each case. The strength of the signal is highest for homeopathic medicine prepared from Plumbum Metallicum followed by Ferrum Metallicum, Antim Tart, Alumina, Argentum, and Zincum Metallicum. The strength of the magnetic signal was lowest for the Zincum Met medicine. The variation of the electromagnetic signals in different samples may be related to the nature of metal properties used to prepare the homeopathy medicine. It is very interesting to note that Plumbum Metallicum contains the Pb element which is a heavy element (High atomic mass of metals compared to other homeopathy samples) and it usually shows the diamagnetic property, however, in the present investigation its homeopathy sample shows high electromagnetic signal.
Further, Ferrum Metallicum medicine contains the Fe element which is ferromagnetic in nature and thus exhibits high electromagnetic signal than other medicine samples except for Pb-based homeopathy samples. Antim Tart medicine is prepared with antimony and potassium metals, where antimony usually belongs to the diamagnetic and potassium as the ferroelectric class of materials. Therefore, observation of significant electromagnetic signals from Antim Tart is may be due to their coupled magnetic properties. The aluminum-based homeopathy medicine shows a lower magnetic signal than Antim tart homeopathy medicine, which is ascribed due to the paramagnetic properties of the Al element. Further, an electromagnetic signal of 148.4 and 148.3 µV were observed from Argentum met and Zinc met-based homeopathy samples, respectively. These values are very close and lower than other electromagnetic signals observed from another homeopathy medicine. Such behavior is due to their diamagnetic properties and almost similar value of magnetic susceptibility (χm) of the Ag (~ 19.5 x 10−6 cm3 mol−1) and Zn (-9.15 x 10−6 cm3 mol−1) elements which are used to prepare their homeopathy medicine41. It is worth pointing out that although various homeopathy samples contained diamagnetic materials but they showed significant electromagnetic signals even under excitation of even low frequency.
Our finding indicates that each of the homeopathic medicine has a different characteristic voltage and during illness, change in the character of bio-photons in the body disturbs the electromagnetic field of a patient. Each of the patients has different micro-voltages/frequency generation from the living organs in the body according to their illness. When the homeopathic medicine is consumed by an ill patient, the photons are released from the base material on interaction with the micro-voltages present in the body. Each of the homeopathic medicine releases the photons at a particular frequency and voltage to bring back the equilibrium in the disturbed bio-photon according to the principle of resonance40.
Therefore, our finding of the existence of the electromagnetic signals from homeopathic medicine under magnetic field will play an important role not only inefficiently detecting and categorizing the homeopathic medicine with different potencies, but it will also help to understand the science behind the mechanism of the pathway of healing due to homeopathic medicines in the human body. Moreover, observation of distinct output voltage from different homeopathic medicine prepared with different potencies confirmed that each potency has different energy levels. Therefore, the degree of homeopathic potencies may also be measured by the indigenously developed system.
The present study also supports the fact that electromagnetic signal generated under low frequency from homeopathic potencies provokes the body’s defense to act or cancels the pathological frequency disturbing the body and therefore curing the disease, as diseases change the electromagnetic properties of the cells, tissues and living organs of a biological system.
3.2 Raman Measurements: Raman spectroscopy provides a powerful tool to characterize the structure of various kinds of materials, structural changes and it is widely used to characterize the defects in the materials. Various information such as interactions among the functional groups, chain orientation, and their interfacial properties can be also revealed from the Raman spectroscopy analysis. In the present study, we have recorded the Raman spectra (Figure 7) of FM potencies prepared with α‑lactose monohydrate as its base, samples under wavenumber region of 100-1400 cm −1. Various Raman peaks in the low-frequency range at 194, 357, 374, 439, and 475 cm−1 and Raman peak at higher wavenumber at 525, 850, 918,1022, and 1088 cm−1 were detected. The major Raman peaks that appeared at 357, 918 cm−1, 1022 cm−1, and 1088 cm−1 are considered the Raman fingerprints of α‑lactose monohydrate. The potencies with different concentrations of 1X, 2X, 3X, 4X, 5X, and 6X samples also exhibited identical Raman peaks with minor variation and major peaks were of α‑lactose monohydrate (milk sugar). To further observe the changes, we carefully analyzed the Raman of α‑lactose monohydrate and Ferrum Met sample in the same frequency region.
Figure 7. Raman spectroscopy analysis of (a) lactose based and Ferrum Met homeopathic medicines of 1X-6X potency, and (b) enlarged view of the Raman peaks
The Raman peak observed below 500 cm−1 (such as 357, 374, 439, and 475 cm−1) is attributed to the endocyclic vibrations of the ring. The peak at 439 cm−1 could be assigned to the endocyclic C-C-O bending-mode and the band that appeared at 374 cm−1 is due to the C-C-C bending, and a low intense peak was observed in all samples at 357 cm−1 corresponds to the C-5-0-5-C-1. Moreover, the sharp Raman lines observed at 1120 and 1088 cm−1 are related to the D-glucose (lactose) and D-fructose, which occurs due to the C-OH bending-mode and the C-O stretching mode. The band that occurred at 474 cm−1 may be assigned to the C-C-C bending modes of the o-fructosyl moiety, whereas 439 cm−1 could be assigned to the C-C-C mode of the D-glucosyl moiety. The perturbation and slight change in the peak intensity at various bands could probably be explained by the change in the concentration, which is associated, with the hydrogen bonding, of the OH groups. The Raman peak observed at 918 cm−1 frequencies can be assigned to the bending of C-H, which was found in the D-glucose and D-fructose and related to the deformation of C-1-H in D-glucose. Another characteristic vibration of this linkage is the C-O-C bending-mode, which is appeared at 374 cm−1 and is associated with the spectra of D-glucose and D-fructose. It is interesting to note that a sharp peak at 876 cm−1 was detected from the potencies (1X - 6X) sample. However, this peak does not appear in the lactose sample. As discussed in the previous section, Ferrum potencies are used with the lactose samples to prepare the homeopathic medicine. Due to the standard sample preparation procedure, the concentration of the Ferrum (Fe2O3) was very less and it was very difficult to detect the intense peak in the Raman spectra. However, a high-intensity peak near 876 cm−1 was detected in the Raman spectra of potencies (1X to 6X). Few other very minor intense peaks at 173, 189, 257, 286, and one small intense peak at 701 cm−1 are detected. Fe2O3 belongs to the hematite and R-3c crystal space group. Usually, in Raman spectra, two A1g phonon modes and five Eg phonon modes are expected.
In the present case, the peaks at 173, 189 cm−1 are associated with the A1g phonon mode, and the observed peak at 173, 189 cm−1 corresponds to T2g modes while the peaks at 289 and 701 cm−1 is related to Eg phonon modes. Surprisingly, an intense peak as detected at 876 cm−1 is not directly related to the iron particles, however, the existence of this peak may be understood by considering the effect of oxidation of magnetite while performing the Raman experiments. A similar observation was also reported in the previous study [reference missing], and it was reported that such peaks are observed due to the formation of residual iron oxides during the magnetite formation the phase transformation of magnetite. The changes in the Raman intensity and peak position are related to the broken hydrogen bonds that act as medicinal memory in highly diluted medicine in different potencies, thought to play an important role in the pathological healing pathways, through the generation of the associated voltage in the human body.
3.3 FT-IR analysis: The Fourier transform infrared spectroscopy (FT-IR) is a technique that is used to characterize the functional group of inorganic and organic compounds which are the key signatures of materials. In the present work, FT-IR spectra of homeopathic medicines prepared with α‑lactose monohydrate as base material are used. FT-IR of the lactose and potencies medicine prepared from 1X to 6X were measured under a range of 450-4000 cm−1. Figure 8 shows the FT-IR spectra of the homeopathic medicines and the lactose base. Several peaks related to lactose are detected in the FT-IR sector. The peak detected at 3528 cm−1 indicates the free –OH groups belonging to the terminal end of the structure of lactose. The absorption band that occurred at 2898 and 2933 cm−1 reveals the vibration of CH2. An absorption peak at 1651 cm−1 is associated with the -OH stretching of adsorbed water. It is well known that lactose and other carbohydrates easily absorb water from the atmosphere as they contain numerous -OH groups, which are highly polar and hygroscopic. The band 1255 - 1021 cm−1 indicates the C=O frequencies 42. The absorption band appeared between 1021 -745 cm−1 were assigned to CO and CC stretching of CH deformation followed by CH2 vibration43, 44.
Figure 8. FT-IR of the homeopathic medicine showed (a) the presence of all measure peaks related to lactose sample and an undetectable Ferrum (Iron Oxide), and (b) enlarged view of the FT-IR peaks
Moreover, the band 2900-3450 cm−1 was assigned to CH and OH vibrations groups. The band from 600 to 1500 cm−1 reveals the C-O and C-C groups of vibration of carbohydrates. The absorption band located at 900–750 cm−1 is characteristic of the saccharide configuration of lactose. It is worth pointing here that no additional peaks are observed in the case of homeopathic potencies made from Ferrum Metallica, slight changes in the peak intensity were only observed. To further confirm the presence of metal oxide in the samples, we have enlarged the FT-IR spectrum in the range of 750-500 cm−1. It is well known that metal oxide peaks usually have signature peaks in the range of 750-500 cm−1. The enlarged view of the curve represents the presence of all major peaks related to the lactose sample. The FT-IR result confirmed that the presence of Ferrum (iron oxide) in these samples is not detectable through these techniques. However, a significant change in different potencies is clearly visible in the Raman spectra.