Foliar-Applied Selenium Nanoparticles Alleviate Cadmium Stress Through Changes in Physio-Biochemical Status and Essential Oil Profile of Coriander (Coriandrumsativum L.) Leaves

doi:10.21203/rs.3.rs-1108213/v1

Since large areas of agricultural soils around the world are contaminated by Cd, a cost-effective and practical method is needed for the safe production of edible plants. The effective role of many nanomaterials to improve plant yield and alleviate environmental pollutions is addressed; however, selenium nanoparticles (Se-NPs) potential has not been well-known yet. The aim of this work was to investigate foliar application of Se-NPs on yield, water content, proline concentration, phenolic content, lipid peroxidation, and essential oil (EO) attributes of coriander (Coriandrum sativum L.) under Cd stress. This study consisted of Cd contamination (0, 4, and 8 mg L^-1) and foliar application of Se-NPs (0, 20, 40, and 60 mg L^-1) in a hydroponic system. The results showed enhanced Cd concentration of root and shoot of coriander under Cd stress; however, Se-NPs alleviate the uptake of Cd. There were observed the decreased shoot and root weight, chlorophyll (Chl) content and relative water content (RWC) by progressing Cd toxicity, but Se-NPs improved these attributes. Moderate Cd stress and 40 or 60 mg L^-1 Se-NPs increased phenolic and flavonoid contents as well as EO yield. Proline concentration and malondialdehid (MDA) increased by enhancing Cd stress, but Se-NPs decreased MDA. The GC/MS analysis showed that the main EO constitutes were n-decanal (18.80-29.70%), 2E-dodecanal (14.23-19.87%), 2E-decanal (12.60-19.40%), and n-nonane (7.23-12.87%), representing different amounts under Cd pollution and Se-NPs. To sum up, 40-60 mg L^-1 Se-NPs are effective in alleviating Cd stress.

Essential oil composition

Heavy metals

Nanotechnology

Phenolic content

Environmental pollution due to trace metals is a main concern on agricultural soils, which threatens food security (Hussain et al. 2020). Despite plants require some microelements like Se, Cu, Co, Zn, and Fe, others such as Pb and Cd are virulent to plants (Rizwan et al. 2019). Cd is addressed as the most harmful element for animals and humans (Khosropour et al. 2019). Cd can release in soil environment through different anthropogenic actions like mining wastes, sewage sludge, synthesis fertilizers, and industrial waste (Hamid et al. 2019; Hussain et al., 2020). There is an increasing contamination of Cd in developing countries due to noticeable urbanization and industrialization (Hussain et al. 2020). Plants uptake heavy elements from soil and accumulate in their tissues, and thereby enhance the health risk for humans through consuming the edible parts particularly grains of crop (Gall et al. 2015). Plants adjust their physiological and molecular processes to reach the normal growth when exposed to Cd stress (Wang et al. 2020). Cd enhances the oxidative damages in plants by stimulating the production of the reactive oxygen species (ROS), where the overproduction of ROS leads to the photosynthesis damage and growth reduction through disturbing the plant defense system (Rizwan et al. 2019).

Nanoparticles (NPs), as a main part of nanotechnology, has received increasing attention on mitigating the contamination rate of the environment (El-Saadony et al. 2021) and thereby to improve human life (Fatemi et al. 2021). NPs are considered as effective materials in the field of nutrition, because they have high adsorption capacity and large surface area (Fatemi et al. 2021). Regarding their shape, size, and application method, NPs have different impacts on plants (Rastogi et al. 2019). Since the diameter of stomata pores is ~10 nm, NPs can easily penetrate through this gate under foliar spray techniques (Singh et al., 2020). After penetration and translocation, NPs induce the physiological and biochemical changes of plants which can either beneficial or harmful (Singh et al. 2020). They can eliminate ROS generated under abnormal conditions such as heavy metals (Rubio et al. 2019). Recently the NPs form of metals have been widely used in agriculture. Selenium (Se) is a beneficial and essential micronutrient for humans and animals, which acts as the cofactor and coenzyme in improving the immune system of human body (Ikram et al. 2021). According to the world health organization (WHO) reports, around 15 percent of the global population suffer from Se deficiency (Tan et al. 2016; Husain et al. 2020). In addition, the positive effects of Se on plants have been recently reported in some plants during biotic and abiotic stress such as drought (Rady et al. 2020; El-Saadony et al. 2021), salinity (Zahedi et al. 2019; Sheikhalipour et al. 2021; Soleymanzadeh et al. 2020) and heavy metals (Zhao et al. 2019; Wu et al. 2020; Zahedi et al. 2021). In plants under the interaction of Cd and Se application, Se usually mitigates Cd toxicity through subsiding oxidative stress, forms an insert complex or reduces Cd uptake and rehabilitees damaged cells (Hussain et al. 2020; Wu et al. 2020). Vital biological processes are exerted by Se through activating selenoenzymes, which can boost antioxidant capacity, fortify ROS scavenging, and protect cell membrane (Hussain et al. 2020).

Coriander (Coriandrum sativum L.) is an herbaceous plant of the Apiaceae family. It can grow between 30 to 120 cm depending on the growth conditions such as temperature, water, soil etc. Coriander is widely used as a vegetable and in food preparation (Amiripour et al. 2021). In addition to giving a pleasant taste and smell to food, coriander also has a high nutritional value (Memari-Tabrizi et al. 2021). Recently, three is an interest to use the coriander as medicinal plants due to its high quality of fatty acids (Amiripour et al. 2021) and valuable essential oil (EO) compounds (Memari-Tabrizi et al. 2021), which has a noticeable role on controlling a broad range of human diseases such as skin and digestive problems (Wei et al. 2019).

Recently, NPs have been emphasized as useful mechanisms for enhancement in yield of plant products. The beneficial role of NPs in the presence of heavy metals have been explained on coriander plants (Fatemi et al. 2021; Cordoba et al. 2021; Afshari 2021). However, there is a few reports about participation of Se-NPs in the mitigation of Cd stress in terms of biochemical variations in coriander. Accordingly, the objectives of this study were (1) to explore Cd concentration in plan tissue, leaf water and chlorophyll (Chl) content, proline and malondialdehid (MDA) concentration, and phenolic content with Se-NPs spray and Cd stress, (2) to quantify and qualify leaf EO of coriander plants in the presence of Cd and Se-NPs, and (3) to find the best level of Se-NPs for subsiding the undesirable function of Cd in coriander. These results can discover appropriate levels of Se-NPs to control the Cd stress on coriander plants in terms of optimum yield and EO composition.

Growth conditions

The coriander seeds were obtained from the Pakan Bazr company, Isfahan, Iran. The experiment was carried out in a greenhouse with a relative humidity of 65%-75% and a photoperiod of 16/8 (lightness/ darkens) in Islamic Azad University of Maragheh, Iran. The growing medium was cocopeat and perlite at a ratio of 2:1, which was applied in three-liter pots.

Experimental design and treatments

The Cd stress and Se-NPs, as treatments of the study, was carried out in a factorial based on completely randomized design (RCD) with three replicates. Each pot contains one plant of coriander. Three different Cd stress were 0 (control), 4 mg L^-1, and 8 mg l^-1 applied at 4-leaf growth stage. The irrigation with Cd solution (250 mL in each pot) was occurred for five times.

Se-NPs (Sigma-Aldrich, USA) were spherical with average particle size of 10-40 nm, specific surface area of 30-50 m² g⁻¹, true density of 3.89 g cm⁻³, CAS number of 7446-08-4, and purity of 99.9%. The Se-NPs at levels of 0 (control), 20, 40, and 60 mg L^-1were sprayed on plants two times in 6-leaf stage (first time) and after 15 days (second time). After the flowering stage, the leaves were sampled to measure the physiological and biochemical attributes.

Cd concentration

ICP/MS (Agilent 4500 series, USA) was used to measure Cd concentration in coriander tissues. The 0.2 g of dry matter was mixed with 4 ml of 65% nitric acid (NO2) and kept at 25 °C for 24 hours. Thereafter, the sample was placed in an oven at 90°C until evaporating NO₂. The volume was made up to 10 ml with distilled water (Khosropour et al. 2019).

Relative water content (RWC) determination

The developed leaves were applied to measure RWC. After measuring the fresh weight (FW), saturation weight (SW) was obtain by immersing the leaves in distilled water for 24 hours. After that the leaf samples were dried at 70°C for 24 hours to get their dry weight (DW). Eventually, RWC was calculated based on the following equation (Dhopte and Manuel 2002).

Malondialdehyde (MDA) measurement

To determine MDA, 0.2 g fresh leaf tissue was grinderd with 5 mL of trichloroacetic acid (TCA, 1%) and centrifuged for 5 min at 10,000 rpm. Moreover, 5 mL of TCA (20%) containing 0.5 mL thiobarbituric was added to 1 ml of supernatant and placed in a 95 °C water bath for 30 min, and followed by centrifuging for 10 min at 10,000 rpm. The absorbance wavelength was 520 nm (Heath and Packer 1968).

Chlorophyll (Chl) assay

To determine Chl content, 0.5 g of leaf tissue was mixed with 4 ml of acetone (80%.) The supernatant was prepared after centrifuging at 4000 rpm for 5 min. To measure total Chl, a spectrophotometer (T80 model) was calibrated and zeroed using acetone 80% in 630, 647, and 664 nm wavelengths. The absorbance of the solutions was read and the concentration Chl was calculated (Arnon 1949).

Proline concentration

To determine the proline content, 0.1 g of fresh leaf tissue was mixed with 10 ml of sulfosalicylic acid (3% w / v) and centrifuged at 4000 xg for 20 min. Then, the mixture was supplied with 2 mL of ninhydrin acid and 2 mL of glacial acetic acid. Simultaneously, 2 ml of standard 0, 4, 8, 12, 16, 20 mg of proline were mixed with 2 ml of ninhydrinic acid and 2 ml of acetic acid. The samples were read at 520 nm using a spectrophotometer (Bates et al. 1973).

Total phenolic content (TPC)

The TPC was measured with Folin-Ciocalteu as a reagent and gallic acid (GA) as a standard. For this, 2 g of leaf sample was homogenized with 8 ml of 80% ethanol and centrifuged at 12000 × g for 20 min. After that, 0.5 mL of supernatant was transferred into 15 mL Falcons. Then 500 μl Folin-Ciocalteu was added to mixture and after 2 min, 1 mL sodium carbonate (7%) was added to the reaction mixture and the final volume was reached to 6 mL using distilled water. The mixture was remained in a 30 ° C (dark condition) bath for 90 min. The absorbance of the samples was measured in a 725 nm wavelength with a spectrophotometer (McDonald et al. 2001).

Total flavonoid content (TFC)

The amount of TFC was measured by aluminum chloride colorimetric method. In this method, 0.5 mL of the extract solution with mixed with 1.5 ml of 95% ethanol, 0.1 ml of 10% aluminum chloride, 0.1 ml of 1 M potassium acetate and 2.8 ml of distilled water. After keeping the samples at room temperature for 30 min, the adsorption of the mixture was read at 415 nm. The quercetin standard was used to draw the curve (Chang et al. 2002).

Essential oil (EO) content and composition

To obtain EO content, dried leaves were hydro-distillated for 3 h using a Clevenger-type apparatus (European Pharmacopoeia, 1983). The EO samples were dehydrated by placing them in dark glass bottles containing anhydrous sodium sulfate. The samples were maintained at 4 º C until they were analyzed by gas chromatography and/or mass spectroscopy. EO yield was determined as the amoun of EO extracted from total dry weight per pot. The EO compisition were analysed by Varian 3400 GC-MS system equipment with AOC-5000 auto injector and DB-5 fused silica capillary column (30 m × 0.25 mm i.d.; film thicknesses 0.25 µm).

Data analysis

All data was statistically analyzed SAS software in three replicates. The value of treatments was compared by Duncan’s multiple range tests. The data were statistically investigated at 5% probability level.

Cadmium (Cd) concentration in root and shoot

The increased Cd concentrations in roots and shoots of coriander plants were observed when exposed to Cd stress, but Se-NPs reduced Cd content in these tissues. About 2.5-fold increase of Cd content in shoot and root were obtained in plants experiencing 8 mg L^-1 Cd relative to 4 mg L^-1 Cd. Under severe Cd stress (8 mg L^-1 Cd), 20, 40, and 60 mg L^-1 Se-NPs decreased the Cd concentration of roots by 20, 60, and 50 % compared with non-foliar applied plants (Fig. 1a). The decline of Cd concentration in shoot (Fig. 1b) were 17, 64, and 61% for 20, 40, and 60 mg L^-1, respectively. Although all levels of Se-NPs prevented the uptake of Cd in coriander plants, the effect of 40 mg L^-1was more effective.

Shoot dry weight (SDY) and root dry weight (RDW)

The SDY and RDY significantly (P≤0.05) decreased by Cd stress over control; however, Se-NPs improved these attributes. Under severe Cd stress, 40 and 60 mg L^-1 Se-NPs increased SDW by ~50% when compared to control (Fig. 1c). Like SDW, RDW showed the significant enhancement when plants sprayed with Se-NPs at Cd-stressed conditions. Under non-foliar application of Se-NPs, 4 and 8 mg L^-1 Cd decreased RDW by 24 and 50% as compared to control (Fig. 1d).

Relative water content (RWC) and chlorophyll (Chl) content

The remarkable increase of RWC was observed at non-stressful treatment when plants were sprayed with 40 and 60 mg L^-1Se-NPs. The main changes of RWC was corresponded to Cd stress so that the minimum amount was reported in plants exposed to 8 mg L^-1 Cd and non-foliar application of Se-NPs (Fig. 2a). Like RWC, Chl content decreased with Cd stress but increased with foliar application of Se-NPs. At non-Se application, 34% reduction of total Chl content was obtained in plants exposed to 8 mg L^-1 Cd (Fig. 2b).

Proline and malondialdehid (MDA)

Proline concentration increased with Cd and Se-NPs, but the effect of Cd stress was stronger in increasing the proline accumulation. At non-sprayed plants, 8 mg L^-1 Cd increased proline concentration by 73% when compared with control. The role of Se-NPs was highlighted in low Cd-stressed plants (Fig. 2c). In addition, Cd stress increased MDA, while Se-NPs decreased it. The highest MDA (34.1 nM g^-1FW) was observed in plants exposed to severe Cd stress with non-foliar application of Se-NPs. Under 8 mg L^-1 Cd, the 55% decline of MDA was recorded when plants spayed with 60 mg L^-1 Se-NPs (Fig 2d).

Total phenolic content (TPC) and total flavonoid content (TFC)

TPC and TFC significantly increased with Se-NPS. However, their amounts were different under Cd stress. The highest amount of TPC (Fig. 3a) and TFC (Fig. 3b) was recorded in 4 mg L^-1 Cd and when plants were sprayed with 40 or 60 mg L^-1 Se-NPs. In plants treated with 60 mg L^-1 Se-NPs, moderate Cd stress (4 mg L^-1) enhanced TPC and TFC by 41 and 19%, respectively, compared with control. However, severe Cd stress decreased TPC and TFC relative to the non-stressed treatment. On the other hand, Se-NPs improved the antioxidant capacity of coriander plants via increasing the TPC and TFC. Under 4 mg L^-1 Cd, 40 mg L^-1 Se-NPs elevated TPC and TFC by 39 and 36% when compared with non-foliar application.

Essential oil (EO) content and essential oil yield (EOY)

The EO of coriander leaves was raised by moderate Cd stress and then decreased under severe Cd concentration. Under 60 mg L^-1 Se-NPs, moderate Cd stress increased EO by 37%, while there was no significant change between control and severe Cd stress on EO content. On the other hand, 40 and 60 mg L^-1 Se-NPs significantly improved EO. Under 4 mg L^-1 Cd stress (moderate stress), 40 and 60 mg L^-1 Se-NPs increased EO by 52 and 58% as compared with non-foliar applied Se-NPs (Fig. 3c). Although there was no significant change of EYO between control and moderate Cd stress, the severe Cd stress remarkably decreased EOY. In plants without Se-NPs, the 2-fold reduction of EOY was observed at 8 mg L^-1Cd relative to control (Fig. 3d).

Essential oil (EO) composition

The GC/MS analysis showed that 24 compounds of EO coriander leaves, which represent more than 97% of total oil for all treatments (Table 1). The main EO constitutes were n-decanal, 2E-dodecanal, 2E-decanal, and n-nonane. The n-decanal decreased by Cd stress and increased by Se-NPs. The minimum amount of n-decanal was reported in severe Cd stress as 15.9%, while its maximum amount was obtained in plants experienced non-Cd stress and 40 mg L^-1 Se-NPs to be 28.7%. However, Cd stress increased 2E-decanal. Under non-foliar application of Se-NPs, severe Cd stress increased 2E-decanal by 31% compared to control. 2E-decanal differed from 14.23% in moderate Cd stress and 60 mg L^-1 Se-NPs to 19.87% in severe Cd stress and 40 mg L^-1 Se-NPs. The n-nonane showed no significant change under Cd stress and foliar application of Se-NPs. It was obtained in a range of 7.23-12.87% of total coriander leaf EO.

Soil Cd contamination is a main restricting factor on medicinal plants through inhibiting plant growth and development and increasing the Cd concentrations in edible parts of plants (Abbas et al. 2017). Coriander is the most eminent vegetable with high medicinal attributes (Amiripour et al., 2021). To minimize Cd accumulation in medicinal plants is a key factor to reduce Cd toxicity and grantee food safety (Memari-Tabrizi et al. 2021). In this work, Cd stress led to accumulation of Cd in coriander tissues, and also this accumulation in roots were higher than shoots. Similarly, compared with shoots, the greater Cd accumulation in roots were reported in basil (Gheshlaghpour et al. 2021) and summer savory (Memari-Tabrizi et al. 2021) under Cd stress. It is well documented that enhanced Cd accumulation leads to acidification of rhizosphere, which can improve Cd bioavailability and mobility. In acidic soil, Cd appeared in free Cd²⁺ ions, but in the lower acidity (pH 6–7), Cd is available in the forms of CdCl⁻, CdHCO₃ and hydrated CdCO₃ (Ismael et al. 2019). Generally, Cd accumulation in aerial parts are lower relative to that in the root (Seregin and Kozhevnikova 2008). Apoplast and symplast are the main routes of Cd translocation due to its high mobility and water solubility (Ismael et al. 2019). Plants due to its phytoremediation potential attempt to accumulate Cd in different parts of plant, subsequently contaminate the food chain and jeopardize human health (Wang et al. 2019). Hence, minimizing Cd uptake and accumulation in edible parts of plants is important. The present study represented the useful role of foliar-applied Se-NPs to mitigate the Cd accumulation of coriander. However, Se-NPs may mitigate Cd accumulation at Cd-stress plants through downregulation of Heavy Metal ATPase (HMA) gene (Greger et al. 2016). The HMA is overexpressed in under hyper-accumulation of Cd in different plant parts (Gheshlaghpour et al. 2021). Recently the foliar application of Se has been widely used in most plants to prevent or mitigate the adverse effects of heavy metals (Zhou et al., 2021). The more appropriate mechanism of foliar Se application relative to soil application has been reported (Ismael et al. 2019).

The SDY and RDY of coriander plants increased by Se-NPs (Fig. 1), which it confirms the beneficial role of Se-NPs in alleviating trace elements stress in plants (Wan et al. 2019; Qi et al. 2021). The restriction of plant growth may be observed due to the Cd accumulation in the tissues via degradation of functional mechanisms, where reactive oxygen species (ROS) is largely generated (Rizwan et al. 2016; Qi et al. 20121). Se-NPs particularly 40 and 60 mg L⁻¹ showed the advantageous role of Se-NPs in ameliorating the Cd uptake. In line with our study, the positive role of Se on plant weight through reducing Cd uptake has been previously reported in different plants (Huang et al. 2017; Hussain et al. 2020). However, the information on nanoparticle form of Se to alleviate Cd stress is low. Tripathi et al. (2016) showed the NPs form of Si more efficiently mitigates heavy metal toxicity than its simple form in maize. Moreover, Qi et al. (2021) showed improvement of Brassica napus L. growth via improving the antioxidant capacity of plants under Cd stress.

The decreased RWC and Chl content under Cd stress were obtained in coriander plants. The RWC is used to assess the water status of plants as the main physiological response to stress (Sarker and Oba 2108). Minimal RWC was observed in plants exposed to high Cd stress (Fig. 2a). The decline in RWC is a strategy for plants to limit certain biochemical processes by preserving the energy needed for survival (Sarker and Oba 2108). The size of Se-NP used in this study was less than 50 nm, and this size was appropriate to penetrate into plant cells (Sabo-Attwood et al. 2012; Taylor et al. 2014; Pérez-de-Luque et al. 2017). Se-NP can increase the water content, thereby improving the biochemical process of plant cells, and finally improving plant growth and performance (Merwad et al. 2018). Like RWC, Chl content increased by Se-NPs but decreased by Cd stress. Under stress conditions, plants reduce photosynthesis rate to remain surviving. The reduced Chl concentration under heavy metals have been reported in different plants (Khosropour et al. 2019; Zhang et al. 2020).

Proline concentration increased over control in plants got Cd stress. Plants change the concentration of proline to regulate cell penetration and protect plants from different types of stress (Bhagyawant et al. 2019). Elevated proline content was observed when Se-NPs were used alone or in interaction with Cd stress. Enhanced proline of coriander leaves is a strategy of these plants to overcome Cd stress. The Se-NPs foliar application and Cd stress increased proline level owing to enhancement in the expression of genes related to proline (La et al. 2019). The results of this study indicate that Cd stress causes proline accumulation, which has been previously confirmed by La et al. (2019) and Bhagyawant et al. (2019). Accumulated proline is a mechanism that removes cellular ROS in order to enhance antioxidant potential by stabilizing cell membranes and enzymes activities in stressful plants (Ashraf and Foolad 2007). Se foliar spray can promote proline by enhancing the activity of glutamyl kinase, which controls proline accumulation in plants (Khan et al. 2015), Similar to our study, Ghasemian et al. (2021) showed that the increased proline content with Se-NPs. Cd stress led to increased accumulation of MDA in coriander plants, whereas Se-NPs reduced its concentration (Fig. 2d). The elevated oxidative stress is due to Cd concentrations in coriander leaves because of oxidative stress induced by Cd stress as well documented in many plant species (Li et al. 2013, 2019; Liu etal. 2019b; Zhu et al. 2019; Wu et al. 2020). Oxidative stress negatively affects metabolic process, which can damage membrane structure in plants (Wu et al. 2020). Lower production of MDA with Se might be because of the Se-NPs role in reducing Cd accumulation in leaves. The decreased MDA with Se application has been reported on wheat plants under Cd stress (Wu et al. 2020).

We observed that moderate Cd stress (4 mg L^-1) lead to enhanced TPC and TFC, but they decreased under severe Cd stress (8 mg L^-1). Under heavy metals, phenolic metabolites play a significant role in scavenging ROS (Khosropour et al. 2019). Similar to our results, Memari-Tabrizi et al. (2021) showed the increased TPC and TFC of summer savory plants under moderate Cd stress (20 mg Kg^-1 soil) and their reduction when experienced high concentration of Cd (20 mg Kg^-1 soil).

Like TPC and TFC, the improved EO content of coriander leaves was observed under moderate Cd stress. In contrast, severe Cd stress imposed the significant decline of EO yield as it can be due to the noticeable reduction of dry weight at severe stress conditions (Memari-Tabrizi et al. 20121). The deleterious impacts of Cd stress were modulated by Se-NPs in terms of improved EO yield (Fig. 3d). Heavy metals can affect EO production in aromatic plants (Tabrizi et al. 20121). The moderate Cd stress along with Se-NPs resulted in the desirable EO yield, which was in line with the results of Memari-Tabrizi et al. (2021) on summer savory because of Si-NPs and moderate Cd stress. The reduced EO content due to severe Cd stress were reported on peppermint (Ahmad et al., 2018) and summer savory (Memari-Tabrizi et al. 2021). The decreased leaf surface and increased the number of EO glands per leaf area leads to increased EO production (Mirzaie et al. 2020). The EO production can be affected by various abiotic stresses through changes in the density of EO glands (Es-sbihi et al. 2020 Farouk et al. 2020). This study addressed the improved EO yield by foliar application of Se-NPs. Microelements like Se and Si may increase EO yield through improvement of ion uptake and cell development (Memari-Tabrizi et al. 2021).

The main EO compounds of coriander leaves were decanal, 2E-dodecanal, 2E-decanal, and n-nonane, which was confirmed by Amiripour et al. (2021) in leaves of coriander plants treated with salinity and silicon. The EO profile was changed with Cd contamination and Se-NPs spraying. Environmental stresses can change EO biosynthesis and EO profile of medicinal plants (Mirzaie et al. 2020). The changes of main EO profile of summer savory have been reported by Memari-Tabrizi et al. (2021) under Cd stress and Si-NPs. We observed the different trends of main EO profile of coriander leaves under Cd stress as compared to salinity stress addressed by Amiripour et al. (2021). Therefore, we can mention that the type is responsible in changing the EO profile of coriander leaves.

Heavy metals mainly Cd are increasing in the agricultural soils and there are many interests to find the effective practice to alleviate their toxicity on plants. The present study showed Se-NPs could mitigate the adverse effect of Cd stress on yield of coriander plants yield through increased RWC, Chl, proline, TPC, TFC, EO content, and decreased MDA. The main variation of EO profile was corresponded to Cd stress. therefore, the management of Cd stress levels and Se-NPs can reach the desirable amount of particular compound of EO composition.

Author contributions Marzieh Babashpour-Asl: writing the initial draft; Elnaz Farajzadeh Memari Tabrizi: conceptualization and methodology; Afsaneh Yousefpour-Dokhanieh³: collecting data and editing the draft.

Data availability: The datasets used and/or analyzed for this work are available from the corresponding author on reasonable request.

Funding Not applicable

Ethical approval Not applicable.

Consent to participate Not applicable.

Consent for publication Not applicable.

Competing interests The authors declare no competing interests.

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Table 1. Essential oil composition of coriander leaves under cadmium stress and selenium nanoparticles

Compound	RI	Non-Cd stress				4 mg L^-1 Cd				8 mg L^-1 Cd
		Se 0	Se 20	Se 40	Se 60	Se 0	Se 20	Se 40	Se 60	Se 0	Se 20	Se 40	Se 60
n-nonane	900	10.23	9.34	9.80	7.23	9.20	9.43	9.93	9.43	11.89	12.87	9.78	10.32
α-pinene	933	0.23	0.15	0.24	0.18	0.32	0.37	0.25	0.17	0.31	0.36	0.37	0.48
n-decane	996	0.56	0.67	0.53	0.62	8.23	0.98	0.78	0.45	0.98	0.56	0.75	1.02
n-octanal	1005	0.56	0.53	0.65	0.34	0.34	0.00	0.54	0.00	0.32	0.00	0.00	0.23
p-cymene	1027	0.00	0.23	0.34	0.27	0.12	1.20	0.00	0.15	0.45	0.76	0.02	0.00
limonene	1030	0.54	0.23	0.34	0.45	0.65	0.23	0.76	0.23	0.16	0.54	0.22	0.47
linalool	1102	0.40	0.67	0.47	1.55	0.90	0.84	0.15	0.33	0.98	0.17	0.19	0.51
n-nonanal	1107	1.54	1.20	1.02	1.65	1.56	1.43	1.22	0.89	1.05	0.00	1.23	2.18
n-decanal	1219	23.90	25.40	28.70	29.70	17.60	22.50	24.70	19.22	15.80	15.90	17.60	16.50
2E-decanal	1273	13.20	14.20	15.20	14.80	13.60	14.60	12.60	15.30	17.34	19.40	16.70	18.30
1-decanol	1277	0.54	1.65	0.76	0.45	2.12	0.54	0.34	0.64	0.76	0.34	0.35	0.60
undecanal	1311	3.12	3.15	2.65	3.43	3.56	4.32	1.45	4.09	2.15	4.21	3.65	3.21
2E-undecanal	1370	3.65	2.76	2.43	4.12	3.50	4.12	3.87	3.70	3.76	2.65	3.12	4.32
dodecanal	1413	6.35	5.20	6.40	5.70	4.80	6.40	6.70	5.20	8.78	7.60	5.40	5.80
2E-dodecanal	1476	16.22	18.03	17.60	14.23	16.70	18.60	15.70	18.42	18.60	17.54	19.87	18.34
(E)-β ionone	1483	0.76	0.23	0.14	0.00	0.87	0.34	0.12	1.45	0.87	0.34	0.43	0.12
tridecanal	1511	0.34	0.65	0.76	0.23	0.05	0.67	0.76	0.05	0.77	0.49	0.72	0.00
E-nerolidol	1560	0.54	0.12	0.87	0.13	1.43	0.67	0.18	0.98	0.45	0.12	0.22	0.78
2E-tridecen-1-al	1572	1.76	1.43	0.95	1.22	1.34	0.15	0.76	0.23	0.34	1.02	1.20	0.00
tetradecanal	1614	1.28	1.28	1.14	1.28	1.78	1.10	1.28	1.28	1.23	1.28	1.43	3.20
2E-tridecen-1-al	1680	8.21	7.12	5.40	7.21	6.50	3.55	9.34	7.12	5.21	7.52	5.60	5.89
neocnidilide	1739	3.12	2.54	2.65	3.76	1.23	3.45	4.34	4.23	5.67	1.50	6.23	4.56
Tridecen-1-al<2E->	1780	2.34	2.34	0.94	1.43	2.76	1.23	2.76	4.37	1.89	3.67	2.87	2.76
Total		99.39	99.12	99.98	99.97	99.16	96.72	98.53	97.93	99.74	98.84	97.95	99.59

Foliar-Applied Selenium Nanoparticles Alleviate Cadmium Stress Through Changes in Physio-Biochemical Status and Essential Oil Profile of Coriander (Coriandrumsativum L.) Leaves

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Abstract

Figures

Introduction

Material And Methods

Results

Discussion

Conclusions

Declarations

References

Tables

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