Laparoscopic surgery has been gradually promoted by people because of its advantages of small trauma and quick recovery. However, the operation is difficult, the first hepatic portal should be completely fastened during the operation. In this paper, through the study of the existing structure of the blocker, a kind of blood flow blocker for the first hepatic hilum blocking under laparoscope is designed. All kinds of parameters were calibrated through equation calculation, and the pressure guiding the blood flow blocking of hepatic portal during operation was calculated. The dynamic analysis was carried out with ANSYS software, and it was found that the fluid movement state was most uniform when the airflow velocity reached 8m/s. The experimental apparatus was set up to simulate the process of hepatic portal vein being blocked in vitro, then the feasibility of blocking effect was evaluated. Finally, it is concluded that the designed blood flow blocking device can have good blocking effect on blood vessels.