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Research
Honggang Liu
Beijing Tongren Hospital
Corresponding Author
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Background
The diagnosis of microinvasive laryngeal squamous cell carcinoma (mLSCC) is not always straightforward and sometimes can be very challenge in daily clinical practice, particularly in the circumstances with differentials such as SIL with inverted growth pattern and PEH. The focus of the diagnosis is the confirmation of microinvasion. Cancer-associated fibroblasts (CAFs), as the major element of tumor microenvironment, are believed to participate actively in the invasion of tumor cells. In this study, we sought to evaluate the diagnostic role of α-SMA labelling CAFs in mLSCC, with comparison of laryngeal squamous intraepithelial lesion (SIL) and benign pseudoepitheliomatous hyperplasia (PEH).
Methods
A total of 81 laryngeal biopsy specimens were retrieved, including 41 cases of mLSCC with depth of invasion no more than 3 mm, 20 laryngeal SIL, and 20 PEH. All tissues were stained for immunohistochemistry, using antibody against the α-SMA antigen. We observed the presence of α-SMA positive fibroblasts in mLSCC, compared the results with that of laryngeal SIL and benign PEH by Chi-square statistics test, and investigated the correlation between tumor histological characteristics and the presence of CAFs in mLSCC.
Results
Immunoreactivity of α-SMA was detected in twenty-nine mLSCC (29/41, 70.7%), while no reactivity was observed in laryngeal SIL (0/20, 0%), and few in PEH (2/20, 10%). The α-SMA expression pattern in stromal fibroblasts of mLSCC were significantly different from those of SIL (χ2 = 26.966, p=0.000) and PEH (χ2 = 19.838, p=0.000). This support that, α-SMA as an immunolabeling of CAFs plays a reliable role in confirming the microinvasion of LSCC. In addition , we finds that there is dense lymphoplasmacytic infiltration in the stroma of a few mLSCC (7/41, 17.1% ) cases rather than tumor-related desmoplastic hyperplasia. And it seems that there is a negative correlation between dense lymphoplasmacytic infiltration and the presence of CAFs in LSCC.
Conclusions
Our study highlights practical role of utilizing α-SMA in the diagnosis of microinvasive laryngeal squamous cell carcinoma, with emphasis on variable histomorphologic features of mLSCCs.
Keywords
α-Smooth muscle actin, Microinvasive laryngeal squamous cell carcinoma, Histomorphologic feature, Pathological diagnosis, Carcinoma-associated fibroblasts
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Honggang Liu
Beijing Tongren Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 24 Nov, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The diagnosis of microinvasive laryngeal squamous cell carcinoma (mLSCC) is not always straightforward and sometimes can be very challenge in daily clinical practice, particularly in the circumstances with differentials such as SIL with inverted growth pattern and PEH. The focus of the diagnosis is the confirmation of microinvasion. Cancer-associated fibroblasts (CAFs), as the major element of tumor microenvironment, are believed to participate actively in the invasion of tumor cells. In this study, we sought to evaluate the diagnostic role of α-SMA labelling CAFs in mLSCC, with comparison of laryngeal squamous intraepithelial lesion (SIL) and benign pseudoepitheliomatous hyperplasia (PEH).
Methods
A total of 81 laryngeal biopsy specimens were retrieved, including 41 cases of mLSCC with depth of invasion no more than 3 mm, 20 laryngeal SIL, and 20 PEH. All tissues were stained for immunohistochemistry, using antibody against the α-SMA antigen. We observed the presence of α-SMA positive fibroblasts in mLSCC, compared the results with that of laryngeal SIL and benign PEH by Chi-square statistics test, and investigated the correlation between tumor histological characteristics and the presence of CAFs in mLSCC.
Results
Immunoreactivity of α-SMA was detected in twenty-nine mLSCC (29/41, 70.7%), while no reactivity was observed in laryngeal SIL (0/20, 0%), and few in PEH (2/20, 10%). The α-SMA expression pattern in stromal fibroblasts of mLSCC were significantly different from those of SIL (χ2 = 26.966, p=0.000) and PEH (χ2 = 19.838, p=0.000). This support that, α-SMA as an immunolabeling of CAFs plays a reliable role in confirming the microinvasion of LSCC. In addition , we finds that there is dense lymphoplasmacytic infiltration in the stroma of a few mLSCC (7/41, 17.1% ) cases rather than tumor-related desmoplastic hyperplasia. And it seems that there is a negative correlation between dense lymphoplasmacytic infiltration and the presence of CAFs in LSCC.
Conclusions
Our study highlights practical role of utilizing α-SMA in the diagnosis of microinvasive laryngeal squamous cell carcinoma, with emphasis on variable histomorphologic features of mLSCCs.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
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