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Research article
Małgorzata Żychowska
Uniwersytet Kazimierza Wielkiego w Bydgoszczy
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9475-0448
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: In this study, we investigated the effects of supplementation and exercise on the expression of genes associated with inflammation like CCL2, CRP, IL1, IL6, IL10 mRNA in elderly women.
Methods: Twenty four participants divided randomly into two groups were subjected to 6 weeks of the same health training program (three times per week). SUP group (supplemented, n = 12, mean age 72.8 ± 5.26 years and mean body mass 68.1 ± 8.3 kg) received 1000mg of Vitamin C/day during the training period, while CON group (control, n = 12, mean age 72.4 ± 5.5 years and body mass 67.7 ± 7.5 kg) received placebo.
Results: No significant changes in IL-1, IL-6, IL-10 and CRP mRNA were observed within and between groups. However, there was a clear tendency of a decrease in IL-6 (two-way ANOVA, significant between investigated time points) and an increase in IL-10 mRNA noted in the supplemented group. A significant decrease in CCL2 mRNA was observed only in the CON group (from 2^0.2 to 2^0.1, p = 0.01).
Conclusions: It can be concluded, that 6 weeks of supplementation and exercise was too short to obtain significant changes in gene expression in leukocytes, but supplementation of 1000mg vitamin C positively affected IL-6 and IL-10 expression – which are key changes in the adaptation to training. However, changes in body mass, IL1 and CCL2 were positive in CON group. It is possible that Vitamin C during 6 weeks of supplementation could have different effects on the expression of individual genes involved in the immune response.
Trial registration: retrospectively registered.
Keywords
supplementation, aging, genes involved in immune response
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CURRENT STATUS: Published
View the published article at Journal of the International Society of Sports Nutrition.
Version 2
Posted 09 Feb, 2021
No community comments so far
Editorial decision: Accept
On 15 Feb, 2021
Review #1 received
Received 14 Feb, 2021
Reviewer #1 agreed
On 08 Feb, 2021
Editor assigned
On 04 Feb, 2021
Reviewers invited
Invitations sent on 04 Feb, 2021
Submission checks complete
On 04 Feb, 2021
Editor invited
On 04 Feb, 2021
No comments provided
Editorial decision: Minor revision
On 26 Jan, 2021
Review #2 received
Received 25 Jan, 2021
Reviewer #2 agreed
On 12 Dec, 2020
Review #1 received
Received 03 Dec, 2020
Reviewer #1 agreed
On 20 Nov, 2020
Reviewers invited
Invitations sent on 20 Nov, 2020
Editor assigned
On 19 Nov, 2020
Submission checks complete
On 19 Nov, 2020
Editor invited
On 19 Nov, 2020
First submitted
On 17 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
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See the published version of this preprint at Journal of the International Society of Sports Nutrition.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Małgorzata Żychowska
Uniwersytet Kazimierza Wielkiego w Bydgoszczy
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9475-0448
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of the International Society of Sports Nutrition.
Version 2
Posted 09 Feb, 2021
No community comments so far
Editorial decision: Accept
On 15 Feb, 2021
Review #1 received
Received 14 Feb, 2021
Reviewer #1 agreed
On 08 Feb, 2021
Editor assigned
On 04 Feb, 2021
Reviewers invited
Invitations sent on 04 Feb, 2021
Submission checks complete
On 04 Feb, 2021
Editor invited
On 04 Feb, 2021
No comments provided
Editorial decision: Minor revision
On 26 Jan, 2021
Review #2 received
Received 25 Jan, 2021
Reviewer #2 agreed
On 12 Dec, 2020
Review #1 received
Received 03 Dec, 2020
Reviewer #1 agreed
On 20 Nov, 2020
Reviewers invited
Invitations sent on 20 Nov, 2020
Editor assigned
On 19 Nov, 2020
Submission checks complete
On 19 Nov, 2020
Editor invited
On 19 Nov, 2020
First submitted
On 17 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of the International Society of Sports Nutrition.
Background: In this study, we investigated the effects of supplementation and exercise on the expression of genes associated with inflammation like CCL2, CRP, IL1, IL6, IL10 mRNA in elderly women.
Methods: Twenty four participants divided randomly into two groups were subjected to 6 weeks of the same health training program (three times per week). SUP group (supplemented, n = 12, mean age 72.8 ± 5.26 years and mean body mass 68.1 ± 8.3 kg) received 1000mg of Vitamin C/day during the training period, while CON group (control, n = 12, mean age 72.4 ± 5.5 years and body mass 67.7 ± 7.5 kg) received placebo.
Results: No significant changes in IL-1, IL-6, IL-10 and CRP mRNA were observed within and between groups. However, there was a clear tendency of a decrease in IL-6 (two-way ANOVA, significant between investigated time points) and an increase in IL-10 mRNA noted in the supplemented group. A significant decrease in CCL2 mRNA was observed only in the CON group (from 2^0.2 to 2^0.1, p = 0.01).
Conclusions: It can be concluded, that 6 weeks of supplementation and exercise was too short to obtain significant changes in gene expression in leukocytes, but supplementation of 1000mg vitamin C positively affected IL-6 and IL-10 expression – which are key changes in the adaptation to training. However, changes in body mass, IL1 and CCL2 were positive in CON group. It is possible that Vitamin C during 6 weeks of supplementation could have different effects on the expression of individual genes involved in the immune response.
Trial registration: retrospectively registered.
Figure 1
Figure 2
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