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Research article
Shuilin He
Fujian Agriculture and Forestry University
Corresponding Author
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Background: Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and have important roles in numerous different physiological processes in plants.
Results: CaCRK5 transcripts were observed to accumulate after the inoculation of R. solanacearum and treatment with salicylic acid. The fusion between CaCRK5 and the green fluorescence protein was targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied by decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 in tobacco conferred increased resistance against R. solanacearum. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation with quantitative real-time PCR analysis verified that a homeodomain zipper I protein CaHDZ27 can bind to the CaCRK5 promoter, and directly active its expression. Yeast two-hybrid and bimolecular fluorescence complementation analyses suggested that CaCRK5 could heterodimerize with the homologous member CaCRK6 in the plasma membrane.
Conclusions: Our data indicated that CaCRK5 played a positive role in pepper resistance against R. solanacearum infection.
Keywords
Capsicum annuum, CaCRK5, Ralstonia solanacearum, Resistance
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Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.docx
Additional file 1. Essential information of CRKs gene family in pepper
- Additionalfile2.jpg
Additional file 2. Photobleaching phenotype of peppers infiltrated with TRV:CaPDS for 15 d.
- Additionalfile3.docx
Additional file 3. The information of all primers
- Additionalfile4.pdf
Additional file 4. Original images for Fig. 6a, Fig. 7a and Fig. 8b
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
Version 1
Posted 01 Dec, 2020
No community comments so far
Review #2 received
Received 20 Jan, 2021
Editorial decision: Major revision
On 20 Jan, 2021
Reviewer #2 agreed
On 30 Dec, 2020
Review #1 received
Received 29 Dec, 2020
Reviewer #1 agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 24 Nov, 2020
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On 18 Nov, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Shuilin He
Fujian Agriculture and Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
Version 1
Posted 01 Dec, 2020
No community comments so far
Review #2 received
Received 20 Jan, 2021
Editorial decision: Major revision
On 20 Jan, 2021
Reviewer #2 agreed
On 30 Dec, 2020
Review #1 received
Received 29 Dec, 2020
Reviewer #1 agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 24 Nov, 2020
Editor assigned
On 22 Nov, 2020
Submission checks complete
On 21 Nov, 2020
Editor invited
On 18 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background: Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and have important roles in numerous different physiological processes in plants.
Results: CaCRK5 transcripts were observed to accumulate after the inoculation of R. solanacearum and treatment with salicylic acid. The fusion between CaCRK5 and the green fluorescence protein was targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied by decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 in tobacco conferred increased resistance against R. solanacearum. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation with quantitative real-time PCR analysis verified that a homeodomain zipper I protein CaHDZ27 can bind to the CaCRK5 promoter, and directly active its expression. Yeast two-hybrid and bimolecular fluorescence complementation analyses suggested that CaCRK5 could heterodimerize with the homologous member CaCRK6 in the plasma membrane.
Conclusions: Our data indicated that CaCRK5 played a positive role in pepper resistance against R. solanacearum infection.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.docx
Additional file 1. Essential information of CRKs gene family in pepper
- Additionalfile2.jpg
Additional file 2. Photobleaching phenotype of peppers infiltrated with TRV:CaPDS for 15 d.
- Additionalfile3.docx
Additional file 3. The information of all primers
- Additionalfile4.pdf
Additional file 4. Original images for Fig. 6a, Fig. 7a and Fig. 8b
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