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Research article
Luming Yang
Henan Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: As an important agronomic trait affecting breeding and consumption choice, the rind color of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] receives considerable attention from breeders. Although rind color occupies an important place in watermelon breeding work, the underlying molecular mechanism governing this complex trait has still not been determined.
Results: In the present study, chlorophyll and carotenoid contents were hypothesized to be the main factors determining rind color. Clearly different structures and numbers of chloroplasts and grana lamellae were found among materials with different rind colors. To study the genes involved in the coloration process of the yellow, dark green, light green, and light green-gray rinds, 84,516 unigenes were obtained from the rind transcriptome. Through DEGs screening and pathway analysis of chlorophyll and carotenoid mechanism, the genes with abnormal expression levels were identified. Then, the nucleotide sequences and expression levels of the putative genes responsible for different rind colors were studied.
Conclusions: This study provides a comprehensive insight on the factors affecting rind coloration and investigated expression levels of the critical genes concerning pigment metabolism in watermelon. These results would help to analyze the molecular mechanisms of rind coloration regulation and development of the related organelles.
Keywords
Watermelon, Rind color, Transcriptomics, Chlorophyll pathway, Carotenoid pathway
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This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xls
Summary of the transcriptome data.
- Additionalfile1.xls
Summary of the transcriptome data.
- Additionalfile2.doc
Functional annotation of the unigenes.
- Additionalfile2.doc
Functional annotation of the unigenes.
- Additionalfile3.doc
Gene Ontology classification of the unigenes.
- Additionalfile3.doc
Gene Ontology classification of the unigenes.
- Additionalfile4.doc
Clusters of EuKaryotic Orthologous Groups (KOG) classification.
- Additionalfile4.doc
Clusters of EuKaryotic Orthologous Groups (KOG) classification.
- Additionalfile5.doc
KEGG classification of the unigenes in C. lanatus.
- Additionalfile5.doc
KEGG classification of the unigenes in C. lanatus.
- Additionalfile6.doc
Correlation of expression levels between RNA-Seq and qRT-PCR.
- Additionalfile6.doc
Correlation of expression levels between RNA-Seq and qRT-PCR.
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Luming Yang
Henan Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: As an important agronomic trait affecting breeding and consumption choice, the rind color of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] receives considerable attention from breeders. Although rind color occupies an important place in watermelon breeding work, the underlying molecular mechanism governing this complex trait has still not been determined.
Results: In the present study, chlorophyll and carotenoid contents were hypothesized to be the main factors determining rind color. Clearly different structures and numbers of chloroplasts and grana lamellae were found among materials with different rind colors. To study the genes involved in the coloration process of the yellow, dark green, light green, and light green-gray rinds, 84,516 unigenes were obtained from the rind transcriptome. Through DEGs screening and pathway analysis of chlorophyll and carotenoid mechanism, the genes with abnormal expression levels were identified. Then, the nucleotide sequences and expression levels of the putative genes responsible for different rind colors were studied.
Conclusions: This study provides a comprehensive insight on the factors affecting rind coloration and investigated expression levels of the critical genes concerning pigment metabolism in watermelon. These results would help to analyze the molecular mechanisms of rind coloration regulation and development of the related organelles.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
Figure 9
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xls
Summary of the transcriptome data.
- Additionalfile1.xls
Summary of the transcriptome data.
- Additionalfile2.doc
Functional annotation of the unigenes.
- Additionalfile2.doc
Functional annotation of the unigenes.
- Additionalfile3.doc
Gene Ontology classification of the unigenes.
- Additionalfile3.doc
Gene Ontology classification of the unigenes.
- Additionalfile4.doc
Clusters of EuKaryotic Orthologous Groups (KOG) classification.
- Additionalfile4.doc
Clusters of EuKaryotic Orthologous Groups (KOG) classification.
- Additionalfile5.doc
KEGG classification of the unigenes in C. lanatus.
- Additionalfile5.doc
KEGG classification of the unigenes in C. lanatus.
- Additionalfile6.doc
Correlation of expression levels between RNA-Seq and qRT-PCR.
- Additionalfile6.doc
Correlation of expression levels between RNA-Seq and qRT-PCR.
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